The QuantStudio 3 and 5 real-time PCR system workflow is supported by the following software applications.

Design & Analysis 1 (DA1) software and Touchscreen Instrument Operating software

This desktop instrument operating software enables users to operate QuantStudio 3 and 5 instruments.  Customers who need to operate the QuantStudio 3/5 system within the desktop software or to access the Securities, Audits, and e-Signature module for the QuantStudio 5 system must use the Design and Analysis v1.x series. 

Get software

Design & Analysis 2 (DA2) software

Design & Analysis 2 software enables users to set up run templates, open completed run files to analyze data, and review results in an integrated and streamlined fashion. The software provides a suite of secondary analysis modules for specific qPCR applications.

While DA2 software can be run with Security, Audit, and e-Signature (SAE) enabled, this feature does not apply to the QuantStudio 3/5 systems.

Get software

If you have purchased a QuantStudio 3 or 5 real-time PCR system, you may download the system software above.

The software is available in a Thermo Fisher Connect folder. To download the software:

  1. Click the Get Software button above, then sign in to Note: If you do not have a account, click Create Account in the sign in screen, then follow the instructions.
  2. After you sign in, the Thermo Fisher Connect folder will open. Locate the software product or documentation of interest, then click download.


The change of firmware and software version may impact validated workflows. You must continue to follow current Instructions for Use (IFU) for validated workflows and/or perform all validations as required by your organization’s standard operating procedures before performing a software upgrade.

For Research Use Only. Not for use in diagnostic procedures.