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No. However, it is a good idea to calibrate all scan settings at least once a year. In this way, if the zoom scan setting is used in the future, it can be reasonably easy to calibrate at that time.

Scan Mode LCQ Fleet Scan Rate (amu/s) LTQ & LXQ Scan Rate (amu/s) LTQ Velos Scan Rate (amu/s) LTQ Velos Pro Scan Rate (amu/s)
Rapid NA NA NA 66.000
Normal 12.500 16.700 33.300 33.300
Enhanced 5.000 5.000 5.000 5.000
Zoom 1.100 1.100 1.100 1.100
Ultra Zoom NA 27 27 27
Turbo 125.000 125.000 125.000 125.000
What does a proper cal mix look like, what should my NL be

The tune file contains four items: Source parameters, ion optic parameters, AGC targets, and scan time settings.

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