Best for reading more than 2,000 samples

These Quant-iT products provide the maximum amount of dye in packaging that is amenable to highly automated quantitation environments. These are the best choice for quantitating more than 2,000 samples at a time.

These Quant-iT products are available in two forms:

  • Concentrated Quant-iT dyes
  • Quant-iT kits with buffer and a standard included

For quantitation of 20–2,000 samples at a time, see our Quant-it Kits for Microplates.

dsDNA specificity of PicoGreen dsDNA Reagent

Figure 1. dsDNA specificity of PicoGreen dsDNA Reagent. Fluorescence enhancement of the Quant-iT PicoGreen dsDNA Reagent upon binding dsDNA, ssDNA, and RNA, demonstrating the strong selectivity of this reagent for dsDNA.

Nucleic acid quantitation using Quant-iT Reagents

Figure 2. Nucleic acid quantitation using Quant-iT Reagents. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation from 20 ng/ml to 1 μg/ml RNA using a 400-fold dilution of the dye; the inset shows the results for 1.0 ng/ml to 50 ng/ml RNA obtained using a 4,000-fold dilution of the dye. (Right) Quantitation of a synthetic 24-mer using the Quant-iT OliGreen ssDNA Reagent is linear over at least four orders of magnitude; the inset shows the results obtained using 0–2.0 ng/ml of oligo.