Figure 1. dsDNA specificity of PicoGreen dsDNA Reagent. Fluorescence enhancement of the Quant-iT PicoGreen dsDNA Reagent upon binding dsDNA, ssDNA, and RNA, demonstrating the strong selectivity of this reagent for dsDNA.
Quant-iT Reagents and Kits for >2,000 Samples
Best for reading more than 2,000 samples
These Quant-iT products provide the maximum amount of dye in packaging that is amenable to highly automated quantitation environments. These are the best choice for quantitating more than 2,000 samples at a time.
These Quant-iT products are available in two forms:
- Concentrated Quant-iT dyes
- Quant-iT kits with buffer and a standard included
For quantitation of 20–2,000 samples at a time, see our Quant-it Kits for Microplates.
Figure 2. Nucleic acid quantitation using Quant-iT Reagents. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation from 20 ng/ml to 1 μg/ml RNA using a 400-fold dilution of the dye; the inset shows the results for 1.0 ng/ml to 50 ng/ml RNA obtained using a 4,000-fold dilution of the dye. (Right) Quantitation of a synthetic 24-mer using the Quant-iT OliGreen ssDNA Reagent is linear over at least four orders of magnitude; the inset shows the results obtained using 0–2.0 ng/ml of oligo.
Expertly detect fluorescence with Thermo Scientific plate readers
High-sensitivity fluorescence detection of nucleic acids or protein for 96-1536 samples can be quickly performed on Fluoroskan or Fluoroskan FL Microplate Fluorometer or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
Featured application: Sensitive DNA quantification
Read about detection limit (LOD) as low as 0.04ng of DNA in this featured application note highlighting sensitive fluorescence detection of DNA capabilities when you combine Quant-iT technology with a Fluoroskan plate reader.
Qubit assays for use with the Qubit fluorometers
For everyday needs when quantitating 1–20 samples, use Invitrogen Qubit Assays for use with the Qubit fluorometers. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. See how easy it is to use the latest version of the Qubit - the Qubit 4 fluorometer.
- Learn more about Qubit assays
For Research Use Only. Not for use in diagnostic procedures.