Fluorescent dyes and non-fluorescent ligands are excellent affinity tags that can be recognized by labeled primary antibodies, providing an alternative to the traditional signal amplification as well as expanding the number of targets detected in multiplex experiments. Applications where anti-dye antibodies have been successfully used include DNA hybridization, immunohistochemistry, flow cytometry, ELISA, and western blotting. Their high specificity also makes them ideal reagents in applications where the signal detection is more difficult due to increased background (for example, non-specific detection of endogenous biotin in cells and tissue).
Anti-dye and anti-hapten antibodies
Catalog # Anti-fluorophore Host Label
Anti-fluorophore antibodies and their conjugates
A5760 Alexa Fluor® 405/Cascade Blue® dye Rabbit None
A11094 Alexa Fluor® 488 dye Rabbit None
A5770 BODIPY® FL dye Rabbit None
A6398 Dansyl Rabbit None
MA514696 Fluorescein (FITC) Mouse (clone #9) None
A6421 Fluorescein/Oregon Green® dye Mouse (clone 4-4-20) None
A889 Fluorescein/Oregon Green® dye Rabbit None
A6413 Fluorescein/Oregon Green® dye Rabbit, Fab fragment None
A11095 Fluorescein/Oregon Green® dye Goat None
A11090 Fluorescein/Oregon Green® dye Rabbit Alexa Fluor® 488 dye
A11096 Fluorescein/Oregon Green® dye Goat Alexa Fluor® 488 dye
A11091 Fluorescein/Oregon Green® dye Rabbit Alexa Fluor® 594 dye
A21250 Fluorescein/Oregon Green® dye Rabbit R-phycoerythrin
A982 Fluorescein/Oregon Green® dye Rabbit Biotin-XX
A21253 Fluorescein/Oregon Green® dye Rabbit Horseradish peroxidase
A5750 Lucifer yellow dye Rabbit None
A5751 Lucifer yellow dye Rabbit Biotin-XX
MA1041 Tetramethylrhodamine (TAMRA) Mouse (clone 5G5) None
A6397 Tetramethylrhodamine (TAMRA)
and Rhodamine Red™ dye
Rabbit None
A6399 Texas Red® and Texas Red®-X dye Rabbit None
Anti-hapten antibodies and their conjugates
MA511251 Biotin Mouse (clone BTN.4) None
033700 Biotin Mouse (clone Z021) None
A6430 Dinitrophenyl Rabbit None
A6435 Dinitrophenyl Rabbit Biotin-XX
A11097 Dinitrophenyl Rabbit Alexa Fluor® 488
A6423 Dinitrophenyl Rabbit Fluorescein
MA135779 Dinitrophenyl Rat (clone LO-DNP-2) None
A21285 Nitrotyrosine Rabbit None
MA112770 3-Nitrotyrosine Mouse (clone 2A12) None


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