Reverse transcription basics

Learn about the roles of reverse transcription in biological systems and in molecular biology research

Reaction setup

Examine essential factors for cDNA synthesis, such as RNA purity, the reverse transcriptase selected, primers used, reaction components, and reaction conditions

Reverse transcriptase attributes

Find out how polymerase activity, RNase H activity, thermostabiliy, and processivity of a reverse transcriptase can impact its functions as a molecular biology tool

Reverse transcription applications

Discover uses of reverse transcription in RT-PCR, cDNA cloning, RACE, gene expression microarrays, and RNA sequencing

Troubleshooting guide

Review troubleshooting tips on reverse transcription with a focus on RT-PCR issues

Reverse transcription videos