Figure 1. Higher, more concentrated yields achieved with the Invitrogen PureLink Genomic DNA Mini Kit.
Genomic DNA Extraction by Sample Type
A full suite of products for all your DNA extraction needs
Isolate and purify high-quality genomic DNA from a wide variety of sample types, including tissue, cells, blood, serum, plants, and forensic samples. Whether you prefer organic reagents, filter columns, or magnetic beads, our DNA purification products are designed for sensitive, scalable extraction and are compatible with a range of downstream applications.
Purify high-quality genomic DNA from a range of sample types:
DNA Extraction From Tissue
Obtaining high-quality, intact DNA is often the first and most critical step in many fundamental molecular biology applications, such as DNA cloning, sequencing, PCR, and electrophoresis. Isolating intact total DNA from tissue samples varies in difficulty with the physical and biochemical nature of the tissue. For example, many DNA extraction methods will work equally well for tissues such as liver, but fibrous tissues such as heart, fatty tissues such as brain, and nuclease-rich tissues like spleen present challenges for DNA isolation. We offer a wide range of DNA extraction from tissue kits, plus the support you may need to get high yields of pure, intact DNA from almost any type of tissue.
Which Genomic DNA Extraction From Tissue Kit is right for you?
Order now | Order now | Order now | Order now |
|
Process the largest amount of tissue | Fast isolation of gDNA from a variety of samples | High -yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
---|---|---|---|---|
DNAzol Reagent | PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | |
Tissue starting material | Up to 50 mg | Up to 25 mg | Up to 25 mg | Up to 25-50 mg |
Yield | Up to 250 µg | 5-10 µg | 5-10 µg | 10-80 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 15 min | 35 min | 45 min |
Prep size | 100 mL | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps |
DNA Extraction From Cells
DNA extraction from cells is one of the most widely used molecular biology techniques. This has traditionally been a highly repetitive and labor-intensive procedure, requiring significant time investment, and often involving the use of toxic organic chemicals. We have developed purification products that are optimized to provide DNA of maximum yield, purity, and integrity from cultured cells, including cell suspensions using several technology formats; organic extraction, silica membranes, and magnetic beads. These options provide users with flexibility, throughput, and choice along both the product and price continuum.
Which DNA Extraction From Cells Kit is right for you?
Order now | Order now | Order now | ||
Process the most cells | Fast isolation of gDNA from cells | High -yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
DNAzol Reagent | PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | |
Cell input | Up to 3x107 cells | Up to 5x106 cells | Up to 5x106 cells | Up to 5x106 cells |
Yield | Up to 70 µg | Up to 10 µg | Up to 10 µg | 15-30 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 15 min | 35 min | 45 min |
Prep size | 100 mL | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps |
DNA Extraction from Blood
Rapid and efficient DNA extraction from blood
Genotyping has become an essential application in clinical trials for pharmacogenomics studies. The emergence of pharmacogenomic centers of excellence has resulted in an increasing need for purification of high-quality genomic DNA from large volumes of blood. Since samples are collected and shipped worldwide, every sample may require different storage and shipping conditions. Choosing the right genomic DNA extraction from blood is important based on your needs and expected end results. We offer a wide range of kits designed for DNA extraction from blood or serum samples at the purity and scale you need.
Which Invitrogen DNA extraction from blood kit is right for you?
Order now | Order now | Order now | Order now | Order now | |
Process the largest amount of blood | Fast isolation of gDNA from blood | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | Fastest automated extraction for blood & saliva | |
---|---|---|---|---|---|
DNAzol BD Reagent | PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | MagMAX DNA Multi-Sample Ultra Kit 2.0 | |
Blood input | 500 µL | 200 µL | 200 µL | 50–350 µL | 50 µL – 2 mL |
Yield | 10–20 µg | 3–10 µg | 3–10 µg | 1.5–16 µg | 1.5–80 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 15 min | 35 min | 90 min using KingFisher instruments | 45 min using KingFisher instruments |
Prep size | 100 mL | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps | 100 preps or bulk reagents |
Viral DNA Extraction
Viral DNA extraction poses unique challenges for good recovery and detection sensitivity. Capture and lysis of virus particles from very dilute solutions or cell-free samples is the first hurdle. We have developed DNA purification products that are optimized to provide maximum viral DNA yield, purity, and integrity from a broad range of sample types in several format options.
- Learn more about viral RNA/DNA purification and detection products
Viral DNA purification from a variety of sample types
Order now | Order now | Order now | |
High-throughput recovery low abundance of viral DNA | Viral DNA purification from a variety of sample types | Consistent recovery of low abundance of viral DNA | |
---|---|---|---|
PureLink Pro 96 Viral RNA/DNA Purification Kit | MagMAX Pathogen RNA/DNA Kit | PureLink Viral RNA/DNA Mini Kit | |
Starting Material | 200uL cell free sample | 50 - 300 uL | 500 µL cell free sample |
Sample Type | Plasma, serum, cerebrospinal fluid | Plasma, serum, whole blood, swabs, feces, ear notches, semen, oral fluid, InPouch™ cultures | Plasma, serum, cerebrospinal fluid |
Protocol Time | 30 min | 45 min | 45 min |
Isolation Technology | Silica Plate | Scalable, flexible format with magnetic beads | Fast, convenient silica column |
High Throughput Compatible | ✓ | ✓ | |
Number of Preps | 384 | 480 | 50 |
Plant DNA Extraction
Plant DNA extraction has unique challenges that require kits specifically designed to deal with carbohydrates, phenolics, and other compounds abundant in plant tissues. Plant cell walls can be very difficult to disrupt, and lysates often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA quality and inhibit downstream reactions. We offer an expansive portfolio of kits, reagents, devices for extraction, purification, analysis, and quantitation of plant DNA. Make your DNA isolation from plant samples easier on you and easier on your samples, while achieving high-yield and high-purity results with our plant molecular biology reagents.
Which Plant DNA Extraction Kit is right for you?
Most cost effective | High quality gDNA at a great value | Fast isolation of gDNA from a variety of plant samples | Rapid & automated extraction of DNA | |
Plant DNAzol Reagent | PureLink Genomic Plant DNA Purification Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX DNA Plant Isolation | |
Plant tissue input | 100 mg | 100 mg | up to 25 mg | up to 100 mg |
Yield | Varies based on starting material | 1–15 µg | 5–10 µg from tissue | Varies based on starting material |
Prep time | 60 min | 40 min | 35 min | 40 min |
Isolation method | Organic extraction | Silica spin column | Filter plate | Scalable, flexible format with magnetic beads |
Automatable | ✓ | |||
High throughput compatibility | ✓ | ✓ | ||
Format | 100 mL | 50 preps | 4 x 96 preps | 96 or 384 preps |
Order now | Order now | Order now | Order now |
Easy, high-yield, high-purity DNA extraction from plant samples
Plant tissue is difficult enough to work with due to the high levels of polysaccharides and polyphenols present. Why compound the issue with tedious, inefficient processes? Cetyl trimethyl ammonium bromide (CTAB) methods require excessive time and handling, limiting your throughput. Silica membrane or magnetic bead–based protocols rely on guanidine and ethanol for DNA capture, but these reagents often don’t remove inhibitors inherent in plant samples that can carry over into the final product and interfere with downstream PCR applications. This leads to frequent sample processing failure, causing you to repeat the purification—if you have the time and sample to spare. We now offer you products specifically designed for easy, high-yield, high-purity DNA purification from plant samples. Inhibitors are removed for reliable downstream results the first time.
FFPE DNA Extraction
Formalin-fixed, paraffin-embedded (FFPE) tissue samples provide a valuable source of stable nucleic acids for gene expression analysis. The clinical utility of FFPE samples is substantial, where retrospective analysis of archival tissue enables the correlation of molecular findings with the response to treatment and the clinical outcome.
Which DNA extraction kit from FFPE samples is right for you?
Order now | Order now | Order now | |
Fast & Easy | Low-throughput isolation of RNA and DNA from the same FFPE section | Scalable isolation of RNA and DNA from the same FFPE section | |
---|---|---|---|
Ion AmpliSeq Direct FFPE DNA Kit | RecoverAll Total Nucleic Acid Isolation Kit for FFPE | MagMAX FFPE DNA/RNA Ultra Kit | |
Prep time | 30 minutes | 4.5 hours (total time for 16 samples) 2.5 hrs hands-on time |
4 hours (total time for 96 preps) 1 hr hands-on time |
Final product extracted | DNA | total RNA, microRNA, gDNA | total RNA, microRNA, gDNA |
Isolation method | lysis | column purification | mag bead purification |
Deparaffinization required | ✓ | ✓ | |
Automation compatible (post deparaffinization) | not applicable | ✓ (Scripts for KF Flex and Duo Prime) |
|
Compatible FFPE sample range | single 5-10 micron section (4-100 mm2 tissue) |
up to 4 x 20 micron sections (300 mm2 tissue) |
up to 2 x 20 micron sections (300 mm2 tissue) |
Sample volume | ~20µL lysate containing DNA | ~50µL purified DNA ~50µL purified RNA |
~50µL purified DNA ~50µL purified RNA |
Compatible with Ion AmpliSeq DNA panels (e.g. Cancer Hotspot Panel v2) | ✓ | ✓ | ✓ |
Compatible with Ion AmpliSeq RNA panels (e.g. RNA cancer and RNA fusion) | ✓ | ✓ |
Why is nucleic acid isolation from FFPE samples such a challenge?
The fixation process causes cross-linkage between nucleic acids and proteins, and covalently modifies adenine and cytidine by the addition of monomethyl groups to the bases. As a result, the molecules are rigid and susceptible to mechanical shearing. Therefore, in order to utilize FFPE tissues as a source for DNA, a reliable method is required for its extraction from the cross-linked matrix.
How can you increase your chances of success with FFPE samples?
There are a number of factors that can impact the overall quality and yield of DNA isolated from FFPE tissues. Here are recommendations to address several key factors:
- Upstream tissue procurement and tissue specimen preparation—if possible, tissues should be fixed within one hour of surgical resection. The optimal fixation time is 12–24 hours, using neutral-buffered formalin or paraformaldehyde. Fixed tissues should be thoroughly dehydrated prior to the embedding process.
- Block storage—storage of blocks without cut faces, when possible, prevents ongoing damage from exposure to atmospheric oxygen, water, and other environmental factors such as light and infestation (fungus, insects, etc.).
- Tissue type, size, and amount being used for RNA isolation—the recommended tissue thickness is 10–20 µm The number of sections used is determined by the tissue type (which impacts cell density) and surface area (recommended size: 50–300 mm2). Excess starting material can cause filter clogging, resulting in poor yield.
- Excessive amount of paraffin used for embedding tissues—when possible, excess paraffin should be trimmed away prior to starting the purification protocol. For xylene-based purification methods, two xylene treatments at room temperature should be sufficient for complete deparaffinization. If desired, a more rigorous 37–55°C treatment can be performed for up to 30 minutes. After the xylene deparaffinization, it is crucial that the 100% ethanol is completely removed and the pellets are dry after the two 100% ethanol washes. The magnetic bead method employs novel chemistries to deal with the paraffin that limits input to 20 µm sections.
The MagMAX FFPE Nucleic Acid (NA) isolation kits are designed for rapid, efficient, and automatable isolation of total RNA and DNA from FFPE samples using MagMAX magnetic particles. MagMAX FFPE NA kits offer faster workflows and use less toxic reagents, without sacrificing quality in the process. These kits help deliver nucleic acid yield and purity comparable to the best-in-class RecoverAll filter-based system. The MagMAX FFPE NA kits outperform products with similar protocols, and achieve RNA and DNA yields that are comparable with longer, more hazardous, and lower-throughput protocols.
The RecoverAll Total Nucleic Acid Isolation Kit procedure requires about 45 minutes of hands-on time. FFPE samples are deparaffinized using a series of xylene and ethanol washes. Next, they are subjected to a rigorous protease digestion with an incubation time tailored for recovery of either RNA or DNA. The nucleic acids are purified using a rapid glass-filter methodology that includes an on-filter nuclease treatment, and are eluted into either water or the low-salt buffer provided.
Buccal DNA Extraction
Buccal cells are one of the most common sample types used to obtain genetic information. Buccal cells are collected by swabbing the inside of a person’s cheek. The cells contained on the swab are then lysed, releasing genomic DNA that can be purified and analyzed.
As with blood samples, buccal swabs have become a standard sample type for genotyping applications in the field of pharmacogenomics. The emergence of pharmacogenomic centers of excellence has resulted in an increasing need for purification of high-quality genomic DNA from buccal swabs. We offer kits optimized for the recovery of DNA from these informative samples.
Which Genomic DNA Extraction From Buccal Swabs Kit is right for you?
Order now | Order now | Order now | Order now | |
Fast isolation of gDNA | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA, recommended for PGx | Fastest automated DNA extraction for high-volume processing | |
---|---|---|---|---|
PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | MagMAX DNA Multi-Sample Ultra Kit 2.0 | |
No. of swabs | 1 swab | 1 swab | 1 swab | 1-2 swab |
Yield | Up to 6 µg | Up to 6 µg | 2-12 µg | |
Isolation method | Silica membrane | Filter plate | Magnetic beads | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ✓ | |
Compatible applications | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 15 min | 35 min | 1 hr | 40 min |
Prep size | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps | 100 preps or bulk reagents |
Bacteria DNA Extraction Kits
Bacteria is present everywhere and as research progresses, we are furthering our understanding of the important role that bacteria play in human health. The study of the microbiome is influencing our understanding of areas such as diabetes, malnutrition, obesity, and women’s health.
Not all bacterial cells are the same, and gram positive samples can be especially challenging to lyse. We have developed a variety of product formats to address all the bacterial cell types from those easy-to-lyse to the more difficult gram positive cells.
Which Genomic DNA Extraction From Bacteria Kit is right for you?
Order now | Order now | Order now | Order now |
|
Most cost effective | Fast isolation of gDNA from bacterial cells | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
---|---|---|---|---|
DNAzol Reagent | PureLink Microbiome DNA Purification Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | |
Cell input | Up to 3x107 cells | Up to 2x109 cells | Up to 2x109 cells | Up to 2x109 cells |
Yield | Up to 70 µg | Up to 25 µg | Up to 10 µg | 15-30 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 45 min | 35 min | 45 min |
Prep size | 100 mL | 50 preps | 4 x 96 preps | 50, 96, 500, 2500 preps |
Forensics DNA Extraction
Forensics DNA samples are often highly variable in quality, making it difficult to validate a single purification protocol that will work for high-throughput screening of various sample types. ChargeSwitch technology is designed to purify samples of variable quality using a single protocol, without centrifugation, and without the introduction of high-concentration salts or organic solvents that can interfere with downstream analytical protocols.
ChargeSwitch Forensic DNA Purification Kit
ChargeSwitch Forensic DNA Purification Kit |
|
---|---|
Recommendation | Optimized for STR and poor or degraded samples |
Protocol Time | <15 min |
Isolation Technology | ChargeSwitch chemistry |
High Throughput Compatible | Yes |
High Throughput/Automation Options | iPrep ChargeSwitch Forensic Kit |
Kit Size | 100 preps |
Order Now |
The ChargeSwitch Forensic DNA Purification Kit affords high sensitivity and robustness for poor or environmentally degraded samples. This kit is fully validated for single tandem repeat (STR) profiling and is compatible with high-throughput protocols. Use the iPrep ChargeSwitch Forensic Kit when using the iPrep Purification System to process your forensic samples.
Rapid, Three Step, pH-Based Purification Protocol
The ChargeSwitch Forensic DNA Purification Kit allows purification of genomic DNA typically in less than 15 minutes following sample preparation, using magnetic beads with a unique, ionizable, nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification is achieved through a simple three-step procedure in aqueous buffers and avoids the use of guanidine, ethanol, and other hazardous reagents.
Validated for Single Tandem Repeat (STR) Profiling
The ChargeSwitch Forensic DNA Purification Kit is suitable for use in STR analysis, for mitochondrial DNA (mtDNA) extraction from hair follicles for single nucleotide polymorphism (SNP) analysis, and includes a flexible protocol to facilitate genomic DNA isolation from a variety of forensic sample types (Figure 1), such as:
- Dried blood spots on paper and clothing
- Hair follicles and hair shafts
- Cigarette butts
- Cigarette paper
- Envelopes
- Drinking vessel swabs
- Chewing gum
- Sperm head cells
- Vaginal epithelial cells
- Door handle swabs
- Strip removed cells (e.g., hats, coats, and gloves)
- "Touch DNA" (e.g., tools, mobile phones, and microscopes)
- Dyed denim.
Figure 1. The ChargeSwitch Forensic DNA Purification Kit effectively purifies DNA from challenging forensic samples. 54 cigarette butts (remains) were collected, 48 samples were purified using the ChargeSwitch Forensic Kit (red bars), 6 samples were purified using a leading competitive kit (blue bars). The DNA was quantified using picogreen and fluorimetric analysis
Figure 2. Average DNA yields by sample type. The standard CST protocol for automated isolation of genomic DNA was used to purify DNA from multiple forensic samples, plus negative controls. All samples were processed in 96-well plates using a Tecan Genesis automated liquid handling robot. The single protocol was found to be highly flexible and could handle the full range of sample types tested.
DNA Extraction From Tissue
Obtaining high-quality, intact DNA is often the first and most critical step in many fundamental molecular biology applications, such as DNA cloning, sequencing, PCR, and electrophoresis. Isolating intact total DNA from tissue samples varies in difficulty with the physical and biochemical nature of the tissue. For example, many DNA extraction methods will work equally well for tissues such as liver, but fibrous tissues such as heart, fatty tissues such as brain, and nuclease-rich tissues like spleen present challenges for DNA isolation. We offer a wide range of DNA extraction from tissue kits, plus the support you may need to get high yields of pure, intact DNA from almost any type of tissue.
Which Genomic DNA Extraction From Tissue Kit is right for you?
Order now | Order now | Order now | Order now |
|
Process the largest amount of tissue | Fast isolation of gDNA from a variety of samples | High -yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
---|---|---|---|---|
DNAzol Reagent | PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | |
Tissue starting material | Up to 50 mg | Up to 25 mg | Up to 25 mg | Up to 25-50 mg |
Yield | Up to 250 µg | 5-10 µg | 5-10 µg | 10-80 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 15 min | 35 min | 45 min |
Prep size | 100 mL | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps |
DNA Extraction From Cells
DNA extraction from cells is one of the most widely used molecular biology techniques. This has traditionally been a highly repetitive and labor-intensive procedure, requiring significant time investment, and often involving the use of toxic organic chemicals. We have developed purification products that are optimized to provide DNA of maximum yield, purity, and integrity from cultured cells, including cell suspensions using several technology formats; organic extraction, silica membranes, and magnetic beads. These options provide users with flexibility, throughput, and choice along both the product and price continuum.
Which DNA Extraction From Cells Kit is right for you?
Order now | Order now | Order now | ||
Process the most cells | Fast isolation of gDNA from cells | High -yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
DNAzol Reagent | PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | |
Cell input | Up to 3x107 cells | Up to 5x106 cells | Up to 5x106 cells | Up to 5x106 cells |
Yield | Up to 70 µg | Up to 10 µg | Up to 10 µg | 15-30 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 15 min | 35 min | 45 min |
Prep size | 100 mL | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps |
Figure 1. Higher, more concentrated yields achieved with the Invitrogen PureLink Genomic DNA Mini Kit.
DNA Extraction from Blood
Rapid and efficient DNA extraction from blood
Genotyping has become an essential application in clinical trials for pharmacogenomics studies. The emergence of pharmacogenomic centers of excellence has resulted in an increasing need for purification of high-quality genomic DNA from large volumes of blood. Since samples are collected and shipped worldwide, every sample may require different storage and shipping conditions. Choosing the right genomic DNA extraction from blood is important based on your needs and expected end results. We offer a wide range of kits designed for DNA extraction from blood or serum samples at the purity and scale you need.
Which Invitrogen DNA extraction from blood kit is right for you?
Order now | Order now | Order now | Order now | Order now | |
Process the largest amount of blood | Fast isolation of gDNA from blood | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | Fastest automated extraction for blood & saliva | |
---|---|---|---|---|---|
DNAzol BD Reagent | PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | MagMAX DNA Multi-Sample Ultra Kit 2.0 | |
Blood input | 500 µL | 200 µL | 200 µL | 50–350 µL | 50 µL – 2 mL |
Yield | 10–20 µg | 3–10 µg | 3–10 µg | 1.5–16 µg | 1.5–80 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 15 min | 35 min | 90 min using KingFisher instruments | 45 min using KingFisher instruments |
Prep size | 100 mL | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps | 100 preps or bulk reagents |
Viral DNA Extraction
Viral DNA extraction poses unique challenges for good recovery and detection sensitivity. Capture and lysis of virus particles from very dilute solutions or cell-free samples is the first hurdle. We have developed DNA purification products that are optimized to provide maximum viral DNA yield, purity, and integrity from a broad range of sample types in several format options.
- Learn more about viral RNA/DNA purification and detection products
Viral DNA purification from a variety of sample types
Order now | Order now | Order now | |
High-throughput recovery low abundance of viral DNA | Viral DNA purification from a variety of sample types | Consistent recovery of low abundance of viral DNA | |
---|---|---|---|
PureLink Pro 96 Viral RNA/DNA Purification Kit | MagMAX Pathogen RNA/DNA Kit | PureLink Viral RNA/DNA Mini Kit | |
Starting Material | 200uL cell free sample | 50 - 300 uL | 500 µL cell free sample |
Sample Type | Plasma, serum, cerebrospinal fluid | Plasma, serum, whole blood, swabs, feces, ear notches, semen, oral fluid, InPouch™ cultures | Plasma, serum, cerebrospinal fluid |
Protocol Time | 30 min | 45 min | 45 min |
Isolation Technology | Silica Plate | Scalable, flexible format with magnetic beads | Fast, convenient silica column |
High Throughput Compatible | ✓ | ✓ | |
Number of Preps | 384 | 480 | 50 |
Plant DNA Extraction
Plant DNA extraction has unique challenges that require kits specifically designed to deal with carbohydrates, phenolics, and other compounds abundant in plant tissues. Plant cell walls can be very difficult to disrupt, and lysates often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA quality and inhibit downstream reactions. We offer an expansive portfolio of kits, reagents, devices for extraction, purification, analysis, and quantitation of plant DNA. Make your DNA isolation from plant samples easier on you and easier on your samples, while achieving high-yield and high-purity results with our plant molecular biology reagents.
Which Plant DNA Extraction Kit is right for you?
Most cost effective | High quality gDNA at a great value | Fast isolation of gDNA from a variety of plant samples | Rapid & automated extraction of DNA | |
Plant DNAzol Reagent | PureLink Genomic Plant DNA Purification Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX DNA Plant Isolation | |
Plant tissue input | 100 mg | 100 mg | up to 25 mg | up to 100 mg |
Yield | Varies based on starting material | 1–15 µg | 5–10 µg from tissue | Varies based on starting material |
Prep time | 60 min | 40 min | 35 min | 40 min |
Isolation method | Organic extraction | Silica spin column | Filter plate | Scalable, flexible format with magnetic beads |
Automatable | ✓ | |||
High throughput compatibility | ✓ | ✓ | ||
Format | 100 mL | 50 preps | 4 x 96 preps | 96 or 384 preps |
Order now | Order now | Order now | Order now |
Easy, high-yield, high-purity DNA extraction from plant samples
Plant tissue is difficult enough to work with due to the high levels of polysaccharides and polyphenols present. Why compound the issue with tedious, inefficient processes? Cetyl trimethyl ammonium bromide (CTAB) methods require excessive time and handling, limiting your throughput. Silica membrane or magnetic bead–based protocols rely on guanidine and ethanol for DNA capture, but these reagents often don’t remove inhibitors inherent in plant samples that can carry over into the final product and interfere with downstream PCR applications. This leads to frequent sample processing failure, causing you to repeat the purification—if you have the time and sample to spare. We now offer you products specifically designed for easy, high-yield, high-purity DNA purification from plant samples. Inhibitors are removed for reliable downstream results the first time.
FFPE DNA Extraction
Formalin-fixed, paraffin-embedded (FFPE) tissue samples provide a valuable source of stable nucleic acids for gene expression analysis. The clinical utility of FFPE samples is substantial, where retrospective analysis of archival tissue enables the correlation of molecular findings with the response to treatment and the clinical outcome.
Which DNA extraction kit from FFPE samples is right for you?
Order now | Order now | Order now | |
Fast & Easy | Low-throughput isolation of RNA and DNA from the same FFPE section | Scalable isolation of RNA and DNA from the same FFPE section | |
---|---|---|---|
Ion AmpliSeq Direct FFPE DNA Kit | RecoverAll Total Nucleic Acid Isolation Kit for FFPE | MagMAX FFPE DNA/RNA Ultra Kit | |
Prep time | 30 minutes | 4.5 hours (total time for 16 samples) 2.5 hrs hands-on time |
4 hours (total time for 96 preps) 1 hr hands-on time |
Final product extracted | DNA | total RNA, microRNA, gDNA | total RNA, microRNA, gDNA |
Isolation method | lysis | column purification | mag bead purification |
Deparaffinization required | ✓ | ✓ | |
Automation compatible (post deparaffinization) | not applicable | ✓ (Scripts for KF Flex and Duo Prime) |
|
Compatible FFPE sample range | single 5-10 micron section (4-100 mm2 tissue) |
up to 4 x 20 micron sections (300 mm2 tissue) |
up to 2 x 20 micron sections (300 mm2 tissue) |
Sample volume | ~20µL lysate containing DNA | ~50µL purified DNA ~50µL purified RNA |
~50µL purified DNA ~50µL purified RNA |
Compatible with Ion AmpliSeq DNA panels (e.g. Cancer Hotspot Panel v2) | ✓ | ✓ | ✓ |
Compatible with Ion AmpliSeq RNA panels (e.g. RNA cancer and RNA fusion) | ✓ | ✓ |
Why is nucleic acid isolation from FFPE samples such a challenge?
The fixation process causes cross-linkage between nucleic acids and proteins, and covalently modifies adenine and cytidine by the addition of monomethyl groups to the bases. As a result, the molecules are rigid and susceptible to mechanical shearing. Therefore, in order to utilize FFPE tissues as a source for DNA, a reliable method is required for its extraction from the cross-linked matrix.
How can you increase your chances of success with FFPE samples?
There are a number of factors that can impact the overall quality and yield of DNA isolated from FFPE tissues. Here are recommendations to address several key factors:
- Upstream tissue procurement and tissue specimen preparation—if possible, tissues should be fixed within one hour of surgical resection. The optimal fixation time is 12–24 hours, using neutral-buffered formalin or paraformaldehyde. Fixed tissues should be thoroughly dehydrated prior to the embedding process.
- Block storage—storage of blocks without cut faces, when possible, prevents ongoing damage from exposure to atmospheric oxygen, water, and other environmental factors such as light and infestation (fungus, insects, etc.).
- Tissue type, size, and amount being used for RNA isolation—the recommended tissue thickness is 10–20 µm The number of sections used is determined by the tissue type (which impacts cell density) and surface area (recommended size: 50–300 mm2). Excess starting material can cause filter clogging, resulting in poor yield.
- Excessive amount of paraffin used for embedding tissues—when possible, excess paraffin should be trimmed away prior to starting the purification protocol. For xylene-based purification methods, two xylene treatments at room temperature should be sufficient for complete deparaffinization. If desired, a more rigorous 37–55°C treatment can be performed for up to 30 minutes. After the xylene deparaffinization, it is crucial that the 100% ethanol is completely removed and the pellets are dry after the two 100% ethanol washes. The magnetic bead method employs novel chemistries to deal with the paraffin that limits input to 20 µm sections.
The MagMAX FFPE Nucleic Acid (NA) isolation kits are designed for rapid, efficient, and automatable isolation of total RNA and DNA from FFPE samples using MagMAX magnetic particles. MagMAX FFPE NA kits offer faster workflows and use less toxic reagents, without sacrificing quality in the process. These kits help deliver nucleic acid yield and purity comparable to the best-in-class RecoverAll filter-based system. The MagMAX FFPE NA kits outperform products with similar protocols, and achieve RNA and DNA yields that are comparable with longer, more hazardous, and lower-throughput protocols.
The RecoverAll Total Nucleic Acid Isolation Kit procedure requires about 45 minutes of hands-on time. FFPE samples are deparaffinized using a series of xylene and ethanol washes. Next, they are subjected to a rigorous protease digestion with an incubation time tailored for recovery of either RNA or DNA. The nucleic acids are purified using a rapid glass-filter methodology that includes an on-filter nuclease treatment, and are eluted into either water or the low-salt buffer provided.
Buccal DNA Extraction
Buccal cells are one of the most common sample types used to obtain genetic information. Buccal cells are collected by swabbing the inside of a person’s cheek. The cells contained on the swab are then lysed, releasing genomic DNA that can be purified and analyzed.
As with blood samples, buccal swabs have become a standard sample type for genotyping applications in the field of pharmacogenomics. The emergence of pharmacogenomic centers of excellence has resulted in an increasing need for purification of high-quality genomic DNA from buccal swabs. We offer kits optimized for the recovery of DNA from these informative samples.
Which Genomic DNA Extraction From Buccal Swabs Kit is right for you?
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Fast isolation of gDNA | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA, recommended for PGx | Fastest automated DNA extraction for high-volume processing | |
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PureLink Genomic DNA Mini Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | MagMAX DNA Multi-Sample Ultra Kit 2.0 | |
No. of swabs | 1 swab | 1 swab | 1 swab | 1-2 swab |
Yield | Up to 6 µg | Up to 6 µg | 2-12 µg | |
Isolation method | Silica membrane | Filter plate | Magnetic beads | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ✓ | |
Compatible applications | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 15 min | 35 min | 1 hr | 40 min |
Prep size | 50, 250 preps | 4 x 96 preps | 50, 96, 500, 2500 preps | 100 preps or bulk reagents |
Bacteria DNA Extraction Kits
Bacteria is present everywhere and as research progresses, we are furthering our understanding of the important role that bacteria play in human health. The study of the microbiome is influencing our understanding of areas such as diabetes, malnutrition, obesity, and women’s health.
Not all bacterial cells are the same, and gram positive samples can be especially challenging to lyse. We have developed a variety of product formats to address all the bacterial cell types from those easy-to-lyse to the more difficult gram positive cells.
Which Genomic DNA Extraction From Bacteria Kit is right for you?
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Most cost effective | Fast isolation of gDNA from bacterial cells | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
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DNAzol Reagent | PureLink Microbiome DNA Purification Kit | PureLink Pro 96 Genomic DNA Purification Kit | MagMAX-96 DNA Multi-Sample Kit | |
Cell input | Up to 3x107 cells | Up to 2x109 cells | Up to 2x109 cells | Up to 2x109 cells |
Yield | Up to 70 µg | Up to 25 µg | Up to 10 µg | 15-30 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ✓ | ✓ | ||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 45 min | 35 min | 45 min |
Prep size | 100 mL | 50 preps | 4 x 96 preps | 50, 96, 500, 2500 preps |
Forensics DNA Extraction
Forensics DNA samples are often highly variable in quality, making it difficult to validate a single purification protocol that will work for high-throughput screening of various sample types. ChargeSwitch technology is designed to purify samples of variable quality using a single protocol, without centrifugation, and without the introduction of high-concentration salts or organic solvents that can interfere with downstream analytical protocols.
ChargeSwitch Forensic DNA Purification Kit
ChargeSwitch Forensic DNA Purification Kit |
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Recommendation | Optimized for STR and poor or degraded samples |
Protocol Time | <15 min |
Isolation Technology | ChargeSwitch chemistry |
High Throughput Compatible | Yes |
High Throughput/Automation Options | iPrep ChargeSwitch Forensic Kit |
Kit Size | 100 preps |
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The ChargeSwitch Forensic DNA Purification Kit affords high sensitivity and robustness for poor or environmentally degraded samples. This kit is fully validated for single tandem repeat (STR) profiling and is compatible with high-throughput protocols. Use the iPrep ChargeSwitch Forensic Kit when using the iPrep Purification System to process your forensic samples.
Rapid, Three Step, pH-Based Purification Protocol
The ChargeSwitch Forensic DNA Purification Kit allows purification of genomic DNA typically in less than 15 minutes following sample preparation, using magnetic beads with a unique, ionizable, nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification is achieved through a simple three-step procedure in aqueous buffers and avoids the use of guanidine, ethanol, and other hazardous reagents.
Validated for Single Tandem Repeat (STR) Profiling
The ChargeSwitch Forensic DNA Purification Kit is suitable for use in STR analysis, for mitochondrial DNA (mtDNA) extraction from hair follicles for single nucleotide polymorphism (SNP) analysis, and includes a flexible protocol to facilitate genomic DNA isolation from a variety of forensic sample types (Figure 1), such as:
- Dried blood spots on paper and clothing
- Hair follicles and hair shafts
- Cigarette butts
- Cigarette paper
- Envelopes
- Drinking vessel swabs
- Chewing gum
- Sperm head cells
- Vaginal epithelial cells
- Door handle swabs
- Strip removed cells (e.g., hats, coats, and gloves)
- "Touch DNA" (e.g., tools, mobile phones, and microscopes)
- Dyed denim.
Figure 1. The ChargeSwitch Forensic DNA Purification Kit effectively purifies DNA from challenging forensic samples. 54 cigarette butts (remains) were collected, 48 samples were purified using the ChargeSwitch Forensic Kit (red bars), 6 samples were purified using a leading competitive kit (blue bars). The DNA was quantified using picogreen and fluorimetric analysis
Figure 2. Average DNA yields by sample type. The standard CST protocol for automated isolation of genomic DNA was used to purify DNA from multiple forensic samples, plus negative controls. All samples were processed in 96-well plates using a Tecan Genesis automated liquid handling robot. The single protocol was found to be highly flexible and could handle the full range of sample types tested.
Compatability
Our products extract high-quality DNA with excellent reproducibility for many fundamental downstream molecular biology experiments, including:
- Next-generation Sequencing
- PCR
- DNA cloning
- DNA sequencing
- DNA electrophoresis
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For Research Use Only. Not for use in diagnostic procedures.