TaqMan® Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold standard assay performance and data quality for pri-miRNA studies.

  • Highly specific – quantitiate miRNA transcription from a single genomic locus
  • Fast, simple, and scalable – two-step RT-qPCR provides high-quality results
  • A new perspective – measure miRNA expression at the gene level

TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic Assays are renowned, to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan® Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and Selection 
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop.

Below is the sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan® Pri-miRNA Assay and TaqMan® MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Catalog # Size # of Reactions/Concentration
4427012 Small 360/20X
4427013 Medium 750/20X
4427014 Large 2900/60X


General specifications

Pre-formulated assay (20x or 60x mix):
  • 2 unlabeled PCR primers (900 nM each final 1X reaction concentration; 150 nM final 1X reaction concentration
  • 1 FAM™ dye-labeled TaqMan® MGB probe (250 nM final 1X reaction concentration)
Documentation: On compact disc:
  • MSDS Formamide
  • MSDS Non-formamide
  • Protocol
  • Quick Reference Card
  • Understanding your Shipment
  • Assay Info File
  • Certificate of Analysis
  • Data Sheet
Printed copies of above documents
Assay Information File: Includes Sales Order Information, List of Contents (Assay ID, Part Number, Expiration Date, & Lot Number), Annotation Information (Gene Symbol, Gene Name, Entrez Gene ID, Transcript Accession & Location on Transcript) and Design Details (Context sequence, Amplicon size)
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes



Shipping and Storage

Shipped at ambient temperature. 
Store at –15ºC to –25ºC. 

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability


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