New SYBR® Select Master Mix | Performance Benchmarking | Related Products
Performance Benchmarking
SYBR® Select Master Mix outperforms the competition with respect to:
- Specificity — specific amplification for 100% of assays
- Brightness — optimum brightness for high performance on most Real-Time PCR instruments
- Sensitivity — true single copy detection as demonstrated with digital PCR
- Dynamic range — more tolerant to high input cDNA
Exceptional Specificity
Out of 44 assays and 3 independent replicate reactions only SYBR® Select Master Mix had a single peak in all the melt curves (100% specificity)
Figure 1 - Percentage of assays with Single Peak in Melt Curve
Brightness that is Just Right
SYBR® Select is brighter than the majority of competitor master mixes, but not too bright to upset some Real-Time PCR instruments
Figure 2 – Average delta Rn (relative fluorescence) across 4 dilution points for PGK1 assay
Spectacular Dynamic Range and Linearity
SYBR® Select produces no NTC signal and is more tolerant to high-input DNA