dsDNA Autoantibodies: SLE Differential Diagnosis and Disease Stratification
Date: Wednesday, July 30, 2025 | Time: 10:30 - 10:55 AM | Booth: 2812
The presence of anti-dsDNA autoantibodies is a key diagnostic criterion for Systemic Lupus Erythematosus (SLE). SLE samples often yield a positive, homogeneous pattern in indirect immunofluorescence assays (IFA) using HEp-2 cells and trigger positive results in ANA screening assays like the EliATM SymphonyS Well. This talk will demonstrate that reflexing positive EliA SymphonyS Well results to the EliATM dsDNA Well and AESKUSLIDESTM nDNA (Crithidia luciliae) IFA assay is a reliable detection and confirmation method. Attendees will learn about the significance of early dsDNA detection, the clinical application by healthcare providers and improvements in laboratory practices to enhance diagnostic value.
Learning Objectives:
- Understand Diagnostic Criteria for SLE: Participants will learn about the importance of anti-dsDNA autoantibodies as a key diagnostic criterion for Systemic Lupus Erythematosus (SLE) and recognize the typical homogeneous pattern produced in IFA testing using HEp-2 cells.
- Implement reliable detection methods: Attendees will gain insights into the effectiveness of reflexing positive EliA SymphonyS Well results to the EliA dsDNA Well and AESKUSLIDESTM nDNA (Crithidia luciliae)* IFA assay as straightforward and reliable approaches for detection and confirmation of SLE.
- Enhance Clinical and Laboratory Practices: Participants will understand the significance of early dsDNA detection, how healthcare providers apply these results clinically, and how laboratory practices can be improved to enhance the diagnostic value and outcomes for patients.