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Invitrogen
Mouse anti-Human IgG Fd Secondary Antibody
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FIGURE: 1 / 2
Human IgG Fd Secondary Antibody (05-4500) in WB
Western blot (non-reducing) was performed using Mouse anti-Human IgG Fd Secondary Antibody (Product # 05-4500) and a 150 kDa band corresponding to Human IgG was observed in IM-9, ARH-77, IM-9 and ARH-77 conditioned medium (CM) but not in Raji, Jurkat, Molt-4 and Jurkat CM. Whole cell lysate (30 µg) of IM-9 (Lane 1), ARH-77 (Lane 2), Raji (Lane 3), Jurkat (Lane 4), MOLT-4 (Lane 5), IM-9 CM (Lane 6), ARH-77 C... View More
Product Details
05-4500
Applications
Tested Dilution
Western Blot (WB)
1:500-1:20,000
Immunohistochemistry (Paraffin) (IHC (P))
1:500-1:20,000
ELISA (ELISA)
1:500-1:20,000
Immunoprecipitation (IP)
1:500-1:20,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Secondary Antibody
Clone
HP6045
Immunogen
Gamma Immunoglobin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG Fd
Antibody Form
Whole Antibody
Product Specific Information
This secondary monoclonal antibody is specially suited for determination of human RF.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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