Secondary antibodies are used for the indirect detection of target antigens. They provide increased sensitivity through signal amplification, and greater flexibility for labeling and detection. We offer an extensive selection of high-quality conjugated and unconjugated secondary antibodies that can be used for the fluorescent, colorimetric, and chemiluminescent detection of primary antibodies in a wide range of applications, such as cell imaging, flow cytometry and western blotting.

Secondary antibody selection table

How to find a secondary antibody

Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.

Popular secondary antibody products

Goat anti-Mouse IgG (H+L) Secondary Antibody
Donkey anti-Rabbit IgG (H+L) Secondary Antibody

Related secondary antibodies applications

Western blot reagents and instruments, chemiluminescent, colorimetric, and fluorescent detection substrates, blocking buffers, and secondary antibody conjugates

We offer a wide selection of ELISA kits targeted to disease-related proteins for research in immunology, cancer, neurodegeneration, stem cells, and more.

Reagents and kits for cellular imaging applications such as fluorescence microscopy, IHC, ISH, HCS, LCM, and small animal in vivo imaging

Antibodies, cell functional reagents, Invitrogen™ Attune™ flow cytometer, and instrument calibration products


Everyone starting out in fluorescence imaging has questions. Learn the fundamentals of fluorescence microscopy, including sample and media considerations, sample preparation, and beginner’s protocols to help you get started. We hope you will benefit from the information in Molecular Probes® School of Fluorescence.