Browse our comprehensive portfolio of conjugated and unconjugated secondary antibodies for fluorescent and chemiluminescent detection of validated* primary antibodies in a wide range of applications, such as cell imaging, flow cytometry, and western blotting.
We offer a wide range of specific target and host species antibody conjugates:
Compare classic, Alexa Fluor and Alexa Fluor Plus fluorophore conjugate technologies and get quick links to different secondary antibody conjugates.
Select from 18 different individual Alexa Fluor dyes and 6 different Alexa Fluor Plus dyes. See superior brightness and better photostability.
Discover features and varieties of available horseradish peroxidase (HRP), alkaline phosphatase (AP), biotin, and other enzyme or affinity-tagged secondary antibody conjugates.
Our HRP (horseradish peroxidase) conjugated antibodies are compatible with chromogenic, fluorogenic, and chemiluminescent substrates. These antibodies provide powerful signal quickly and are well suited for immunohistochemistry (IHC), ELISA, and western blot (WB). We also offer poly-HRP conjugates that provide an optimal number of HRP molecules per conjugated antibody and result in significant signal amplification, impressive specificity, more signal, and incredible signal-to-noise.
Secondary antibody selection table
| Reactivity | Non-fluorescent conjugates | Fluorescent conjugates | Target Ig class |
|---|---|---|---|
Quick links to fixatives, mountants, and other organelle staining reagents for use with secondary antibodies
| Reagent type | Recommended products |
|---|---|
| Mountant | |
| Fixative | |
| Organelle staining |
Advanced Verification
Thermo Fisher Scientific is committed to adopting higher validation standards for the Invitrogen antibody portfolio. We have implemented additional specificity tests to help ensure the highest confidence levels in our products. You can identify the products that have already undergone this testing with the Advanced Verification badge, shown above. This badge can be found in antibody search results and at the top of product webpages. The data supporting the Advanced Verification status can be found in the product specific data galleries. To learn more about our testing standards, please visit Invitrogen Antibody Validation.
Western blot reagents and instruments, chemiluminescent, colorimetric, and fluorescent detection substrates, blocking buffers, and secondary antibody conjugates
We offer a wide selection of ELISA kits targeted to disease-related proteins for research in immunology, cancer, neurodegeneration, stem cells, and more
Reagents and kits for cellular imaging applications such as fluorescence microscopy, IHC, ISH, HCS, LCM, and small animal in vivo imaging
Antibodies, cell functional reagents, Invitrogen Attune flow cytometer, and instrument calibration products
Resources
5 Steps resources
Learning and support
*The use or any variation of the word “validated” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic uses.
