A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.
The Invitrogen antibody portfolio is expanding – see the latest additions
We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Checkout our latest antibody highlights:
Testing the IRF3 Recombinant Rabbit Monoclonal Antibody
The Invitrogen IRF3 recombinant rabbit monoclonal antibody (Cat. No. 703682) was tested in two applications: western blot and immunofluorescence (IF). Specificity was confirmed in IF using cell treatment with a synthetic ligand, resulting in a change in localization of IRF3.
Figure 1. Testing of IRF3 Antibody (3H32L10), ABfinity Rabbit Monoclonal (Cat. No. 703682). (A) Western blotting to detect IRF3 in a panel of mammalian cell lines. (B and C) IF of IRF3 in A549 cells either untransfected (B) or transfected with poly dA:dT (C). Green represents IRF3 signal (Invitrogen Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 (Cat. No. A27034)), blue represents nuclei, and red represents F-actin.
Featured primary antibodies categories
Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.
Learn about our thousands of rabbit, goat, sheep, chicken, and other species of monoclonal primary antibodies.
Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.
Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.
Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.
For Research Use Only. Not for use in diagnostic procedures.