Invitrogen Primary Antibodies

A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.

The Invitrogen antibody portfolio is expanding—see the latest additions

We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Check out our latest antibody highlights:

TBK1 recombinant rabbit monoclonal antibody for neuroinflammation research

TBK1 (TANK*-binding kinase 1) is a member of the IKK subfamily of protein kinases. TBK1 gets activated in response to lipopolysaccharide or viral double-stranded DNA. It leads to receptor-mediated pathogen detection where TBK1 is a modulator of interferon (IFN) levels. It phosphorylates the transcription factor interferon regulatory factor 3 (IRF3), induces its translocation to the nucleus, and stimulates transcription of the genes encoding type-1 IFN. Therefore, TBK1 plays a central role in innate immunity. Recent studies have shown that mutations in TBK1 result in neuroinflammatory/neurodegenerative disorders of the central nervous system, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), normal tension glaucoma (NTG), and childhood herpes encephalitis (HSE). Research into these disorders can benefit from the use of antibodies that are verified for specificity.

*TANK = TRAF family member-associated NF-κB activator
Specificity of monoclonal TBK1 antibody as demonstrated by analysis of relative protein expression and siRNA-mediated knockdown. (A) For immunofluorescence analysis, MCF-7 and SK-BR-3 cells were fixed and permeabilized for detection of endogenous TBK1 using TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) (Cat. No. 703154, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Cat. No. A32731, 1:2,000 dilution). Nuclei (blue) were stained using ProLong Diamond Antifade Mountant with DAPI (Cat. No. P36962), and Rhodamine Phalloidin (Cat. No. R415, 1:800) was used to stain cytoskeletal F-actin (red). Panels a–d clearly demonstrate the nuclear and cytoplasmic localization of TBK1 in MDA-MB-231 cells. Panels e–h show reduced specific staining in SK-BR-3 cells, which have low expression compared to MDA-MB-231 cells, demonstrating antibody specificity. (B) Knockdown of TBK1 was performed in K-562 cells using TBK1-specific siRNA. A decrease in signal intensity was observed by western blot. Densitometric analysis of this western blot is shown in the histogram. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Cat. No. 32209).

Featured primary antibodies categories

Monoclonal Antibodies

Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.

Research Area Antibodies

Find and select primary antibodies for key markers and targets relevant to cancer research, stem cell research, neurobiology research, epigenetic studies, and more.

Polyclonal Antibodies

Learn about our thousands of rabbit, goat, sheep, chicken, and other species of polyclonal primary antibodies.

Flow Cytometry Antibodies

Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.

Rabbit Monoclonal Recombinant Antibodies

Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.

Cell Marker Antibodies

Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.