A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.
The Invitrogen antibody portfolio is expanding—see the latest additions
We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Checkout our latest antibody highlights:
Neurofilaments are the intermediate filament proteins, found specifically in neurons, that are involved in the maintenance of neuronal caliber. They are composed predominantly of three proteins: low (NF-L), medium (NF-M), and high molecular weight (NF-H) neurofilaments. The abnormal accumulation of neurofilaments plays a crucial role in the etiology of many human neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), Alzheimer’s disease, and Parkinson’s disease.
Specificity of Invitrogen NF antibodies. (A) NF-L was observed in the intermediate filaments of the cytoskeleton and neurite extensions in PC-12 cells differentiated to neuronal lineage (panel a), but not in undifferentiated PC-12 cells (panel b). Immunofluorescence analysis was performed using NEFL Monoclonal Antibody (Catalog No. MA5-14981). (B) Relative expression of NF-M was seen in HEK-293 cells (panel a), which were recently discovered to exhibit neuronal properties, but not in HeLa cells (panel b), a null cell model for neurofilaments. Immunofluorescence analysis was performed using NEFM Monoclonal Antibody (Catalog No. MA1-2011). (C) Western blot analysis was performed on a rat brain (lane 1), mouse brain (lane 2), Neuro-2a cell lysate (lane 3), HEK-293 cell lysate (lane 4), HeLa cell lysate (lane 5), K562 cell lysate (lane 6), SH-SY5Y cell lysate (lane 7) and SH-SY5Y cells treated with retinoic acid (10 μM for 48 hours) (lane 8), using NF-H Recombinant Polyclonal Antibody (Catalog No. 711025). Detection was performed using the Novex ECL Chemiluminescent Substrate Reagent Kit (Catalog No. WP20005).
Featured primary antibodies categories
Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.
Learn about our thousands of rabbit, goat, sheep, chicken, and other species of polyclonal primary antibodies.
Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.
Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.
Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.
For Research Use Only. Not for use in diagnostic procedures.