Invitrogen GeneArt Gene Synthesis

With our new Instant Designer gene synthesis tool, we’ve introduced 2 pricing tiers for even better value. Based on your sequence criteria, you’ll have the option to choose within the tool and be guided at each step of the way. Below is a guide to which service level to choose.

* Express and SuperSPEED are available for faster service. For a more detailed see the table below.

Order with confidence

Like all GeneArt Gene Synthesis services, our standard gene synthesis includes the following components to help assure that your construct meets all of your research, quality, and documentation requirements.

If you are new to GeneArt Gene Synthesis, check out our online ordering quick start guide and our gene synthesis tutorials.

It is important to note that genes are made-to-order products and can show variations in producibility, depending on the complexity of the DNA sequence. Typical production times are met for > 90% of genes produced. Production time is now faster by 2 days for genes up to 7 kb and by 9 days for genes between 7–9 kb. Production time is the number of business days required to synthesize genes in our manufacturing facility. Delivery time is in addition to production time and depends on the destination of the shipment.

* Valid for standard gene synthesis (non-complex, GC content 20-80%, repetitions, secondary structures). For complex sequences, production times will be longer and these will be communicated prior to production.
** Subject to sequence assessment. Order must be placed by 3:00 p.m. CET.
Please note: Rarely, requested sequences are found to be toxic and/or genetically unstable. These production times are only valid for nontoxic sequences that are genetically stable in E. coli.

For researchers who need long custom DNA constructs for pharmaceutical development (e.g. viral genomes) or for metabolic engineering, pathway engineering, or synthetic biology, we offer a fast, reliable, and economic solution—our proprietary and completely in-house GeneArt DNA synthesis and assembly services.

• High-throughput  gene synthesis with a capacity of >8.5 Mbp per month (fast and reliable production of DNA pieces for assembly)
• Expert design and assembly technologies and process expertise for the assembly of constructs from 10 kb to several hundred kilobases, with timelines starting from 25 business days
• Robust technologies to build DNA constructs with highest complexity
• Sanger sequencing and next-generation sequencing offer flexible solutions for sequence verification and final QC
• Proven track record of reliable and timely production for long and complex constructs, with dozens of constructs built each year
• Proprietary technologies to build high-quality gene fragments up to 3 kb, and assembly kits that enable do-it-yourself construction and assembly

Learn more about (Sub) genome size and long gene synthesis

• Flexible, custom process: Obtain the exact sequence you need in the vector of your choice—including challenging, complex constructs, and custom vectors.
• Maximized protein yield: Optional sequence processing with GeneOptimizer software generates sequence variants with enhanced mRNA stability and translational efficiency.
• Rapid synthesis and assembly: Standard turnaround times start at 10 business days (accelerated options available).
• Economical: The cost of the GeneArt Gene Synthesis service rivals costs that would be incurred to purchase the oligonucleotides and molecular biology reagents required for gene synthesis, and outsourcing your gene synthesis and cloning means you can focus on your research.
• Excellent accuracy: All custom synthesized genes are sequenced as part of our ISO 9001:2015–certified quality management system; we only ship constructs with exact sequence agreement.
• Multiple options: We can synthesize ready-to-clone linear fragments, synthetic genes in circular plasmids, complete vectors, and more. Compare all gene synthesis options 
• "GeneArt it": Save time and labor by outsourcing your gene synthesis and cloning so you can focus on your research.

Whether you have limited cloning experience or simply want to save time, the GeneArt Gene Synthesis service helps you move your ideas from the planning stage to the laboratory more quickly. Benefit from our experience in successfully producing over 180,000 constructs for customers as diverse as large pharmaceutical companies, biotechnology start-ups, and basic research institutions. The comparison shown below highlights the time and effort saved compared to traditional cloning.

The GeneOptimizer process has been shown to help deliver large increases in protein yield via a combination of factors that stabilize mRNA and maximize translation efficacy (see figure). Efficacy has been proven in a large comparative gene expression study (Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression Fath et al., 2011 PLoS ONE).