Selecting a vector is key to experimental design. Your vector choice is also important for us to know to build your construct quickly and efficiently. Once we synthesize your gene we offer several options for vector delivery designed to provide you with the maximum flexibility and speed of delivery.
Please note: Cloning services are only offered for de novo genes synthesized by the GeneArt Services team.
Options for how to receive your synthesized gene:
- Clone into a basic shuttle vector, pMX
- Clone into a protein expression vector
- Clone into any one of a broad range of vectors, including Invitrogen vectors
- Send us your personal vector
If you’d prefer to start with synthetic linear DNA, we offer affordable and high-quality Strings DNA fragments delivered fast and ready to clone yourself.
Receive a clone in our standard shuttle vector
Our standard shuttle vector, pMX, is a pUC19 derivative with a choice of antibiotic resistance markers. These vectors are intended for delivery of your clonal synthetic DNA. You simply excise your gene via restriction enzymes or PCR amplification and clone as you wish. Any specific flanking restriction enzymes you desire should be included in the gene sequence design provided for de novo synthesis.
Direct cloning into an expression-ready vector
Save up to five days of turnaround time with direct cloning into one of our select Invitrogen vectors, such as pcDNA 3.4 to get your expression-ready genes faster. We offer a range of popular expression vectors for a variety of applications.
Direct expression-ready vectors
Constitutive expression in a variety of mammalian cell lines
Constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression
Production of recombinant baculovirus for expression testing in insect cells
Access to the Gateway Cloning system
|pET100/D-TOPO||Bacterial expression in E.coli|
Saccharomyces cerevisiae expression
To qualify for direct cloning, genes must be <5 kb and must not contain complex sequences. To optimize your gene sequence, reduce complexity, and maximize protein expression levels, use the Invitrogen GeneArt GeneOptimizer.
Express delivery and Super Speed service saves additional production time if you need your clone fast. We offer many options that make it possible to obtain your cloned, expression-ready genes fast with the same high quality you rely on.
Use one of our popular Invitrogen vectors
We can clone your de novo gene into one of the many commercially available Invitrogen vectors that cover a multitude of downstream applications. If you don’t see a vector for selection from the drop-down in the classic ordering portal, you can purchase a commercially available Invitrogen vector and send it to us.
Gateway cloning offers unique benefits
Gateway is a proven and highly cited cloning system that is ideal to shuttle genes of interest between vectors, using site-specific recombination-based cloning. This system offers a simple method to determine an optimal expression system for your research. As the sole provider of the Gateway recombination cloning technology we can subclone your gene into one of our many Gateway vectors.
Gateway offers an easy-to use choice for cloning in multiple expression systems, the insert’s proper orientation and reading frame are maintained during shuttling using the Gateway vectors saving time and effort.
Personalization—using your own vector
We can also subclone your gene into virtually any vector you send us. Simply download the sequence submission form and send to firstname.lastname@example.org and we will respond with a quote for the de novo gene synthesis and cloning into your desired vector.
For Research Use Only. Not for use in diagnostic procedures.