TaqMan Real-time PCR Assays

Applied Biosystems TaqMan real-time PCR assays consist of target-specific primers and one or more probes optimized for specific applications, including gene expression, SNP genotyping, miRNA, mutation detection, and copy number variation analysis.

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TaqMan Assays for every qPCR application

 


Quantitative PCR (qPCR) or real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, supported by intuitive data analysis software, to help harness the power of qPCR across a rich and diverse set of applications.

TaqMan Real-time PCR Assays for Gene Expression, Genotyping, microRNA analysis and other qPCR applications. Available formats include tubes, array cards and plates, microarray or component-level primers and probes.

Gene expression analysis

  • Detect virtually any gene product with our predesigned assays
  • Best-coverage assays to detect highest number of transcript variants possible

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MicroRNA analysis

  • Detect targets with as few as 60 copies of input microRNA (miRNA)
  • Compatible with tissue and biofluids including serum and plasma

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Genotyping

  • Largest selection of predesigned assays for genotyping every SNP of interest as well as polymorphisms associated with drug metabolism (DME)

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qPCR assay and array products

 

 

Assays exactly as you need them:

Predesigned TaqMan Assays

Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. No additional design, optimization, or melt curve analysis needed. Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves—not to mention they’re backed by a performance guarantee (more info below).

We currently offer more than 20 million predesigned TaqMan Assays to address nearly every qPCR application:

2.8 million assays
32   species

1.8 million assays

17 million assays

Custom assay design

Can’t find what you need from our portfolio of predesigned assays? Use our TaqMan Custom Design Assay Tool to easily design your own assay based on your target sequence of interest or submit the primer/probe sequences of your own design. Each custom assay is a mix of forward primer, reverse primer, and FAM and/or VIC dye-labeled TaqMan MGB probe.

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Primers & probes

Design your own TaqMan primer and probe sets from your choice of dye-labeled TaqMan MGB, TAMRA, or QSY custom probes and Applied Biosystems unlabeled, sequence detection primers.

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In your choice of format:

Single-tube TaqMan Assays for qPCR

Whether you select a predesigned assay or custom design your own, you have a variety of sizes - from extra small to large - to choose from to meet the needs of your project.

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TaqMan Array cards & plates for qPCR

Applied Biosystems TaqMan Array cards and plates for real-time PCR contain TaqMan Assays dried down in 96- and 384-well TaqMan Array plates as well as 384-well TaqMan Array microfluidic cards. You have ultimate flexibility when it comes to the content on your cards:

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Fixed content

Preconfigured cards and plates with the most relevant predesigned TaqMan Gene Expression Assays targeting genes for specific biological pathways, processes, or diseases. These cards are inventoried and ready to ship.

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Flexible content

Begin with a suggested list of predesigned assays for each pathway or disease state loaded into a custom configurator that allows for easy modification to fit your needs.

Custom content

Configure your own custom TaqMan Array from scratch using any combination of predesigned assays.

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Specialty arrays

The ultimate in customization. Custom configured cards and plates using one or more custom designed assays along with any combination of predesigned assays.

TaqMan OpenArray

Compatible with the Applied Biosystems QuantStudio 12K Flex system, TaqMan OpenArray simplifies high-throughput qPCR applications. Available in 11 custom formats to suit your throughput needs.

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About
TaqMan Assays

 

 

Applied Biosystems TaqMan Assays are the industry-leading choice for 5' nuclease qPCR assays. They are designed using a highly sophisticated oligonucleotide probe/primer design pipeline.

 

This includes robust primer design algorithms and an extensive array of bioinformatics tools and processes to automate assay design.

Why choose TaqMan Assays?

  • Easy-to-use—more than 20 million assays predesigned and optimized for you
  • Most cited—more than 40K scientific publications
  • Higher sensitivity and precision with MGB probe chemistry

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TaqMan Assay performance guarantee

If a TaqMan Assay doesn't perform as described on our website or datasheet, we'll replace the product at no cost to you or provide you with a credit for a future purchase.

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Additional qPCR applications & products

 

 

  • Non-coding RNA assays

    TaqMan Non-coding RNA Assays provide a highly reliable way to measure expression of long non-coding transcripts. These assays help provide the high specificity and sensitivity you need for quantification and validation studies.

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  • Duplexing with TaqMan Assays

    Duplexing in qPCR is the simultaneous detection of two target sequences in a single reaction. Its key benefits come from minimizing the impact of pipetting errors and maximizing experimental efficiency.

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  • Specialty manufacturing

    If your research application requires special assay manufacturing modifications, such as a different dye or assay volume, or you would like to have your assays plated in a specific way, our custom services can help.

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  • Endogenous control assays

    Pre-designed TaqMan probe and primer sets enable you to normalize the amount of sample RNA or DNA in a reaction. Controls are available for the most common human, mouse, rat, and eukaryotic genes.

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  • Mutation detection assays

    TaqMan Mutation Detection Assays are powered by allele-specific castPCR technology to detect and measure somatic mutations in genes. They are compatible with different sample types, such as cell lines, FFPE tissue samples, and fresh-frozen tissue samples.

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  • Protein expression assays

    TaqMan Protein Assays combine the sensitive, specific protein-binding capabilities of antibodies with the relative quantitation capabilities of 5' nuclease real-time PCR.

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Featured videos

 

 

 

 

Learning and support

 

 

Real-time PCR basics

Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for real-time PCR, we have the learning material, including videos and webinars, to help you understand the technology and get started quickly.

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Real-time PCR Support Center

One resource for all your real-time PCR support needs. Navigate through the qPCR application areas to obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday qPCR problems.

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TaqMan documentation files

Access the files you need for your TaqMan assay, array card, and array plate products.

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