Search Applied Biosystems TaqMan Assays
Quantitative PCR (qPCR) or real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, supported by intuitive data analysis software, to help harness the power of qPCR across a rich and diverse set of applications.
- Detect specific viruses and verify targets involved in the immunological response to viral infection
- Predesigned assays address host genes implicated in SARS-CoV-2 research including viral entry factors, chemokines and other targets
Predesigned TaqMan Assays
Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. No additional design, optimization, or melt curve analysis needed. Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves—not to mention they’re backed by our TaqMan Assay performance guarantee. You can also order your assay with special off-catalog manufacturing modifications using our Specialty service or leverage Thermo Fisher’s manufacturing infrastructure with our GMP oligos optionsor OEM and contract manufacturing services.
We currently offer more than 20 million predesigned TaqMan Assays to address nearly every qPCR application:
Custom assay design
Can’t find what you need from our portfolio of predesigned assays? Use our TaqMan Custom Design Assay Tool to easily design your own assay based on your target sequence of interest or submit the primer/probe sequences of your own design. Each custom assay is a mix of forward primer, reverse primer, and FAM and/or VIC dye-labeled TaqMan MGB probe.
Primers & probes
Design your own TaqMan primer and probe sets from your choice of dye-labeled TaqMan MGB, TAMRA, or QSY custom probes and Applied Biosystems unlabeled, sequence detection primers.
TaqMan Array cards & plates
Individual tubes or tube sets available in XS, S, M, L
96- and 384-well plates with pre-spotted assays
384-well microfluidic cards with pre-spotted assays
3,072 through-holes with pre-spotted assays
High flexibility for low throughput studies
Supports automation for medium-to-high throughput projects
Easy and convenient–no robotics needed
Higher throughput for routine applications or larger projects
Available for both predesigned and custom designed assays.
Offered in a variety of content options:
Available in off-the-shelf, inventoried arrays as well as made-to-order with total flexibility using predesigned and/or custom assays.
Looking to commercialize? Contact our OEM services team today ›
Applied Biosystems TaqMan Assays are the industry-leading choice for 5' nuclease qPCR assays. They are designed using a highly sophisticated oligonucleotide probe/primer design pipeline.
This includes robust primer design algorithms and an extensive array of bioinformatics tools and processes to automate assay design.
Why choose TaqMan Assays?
- Easy-to-use—more than 20 million assays predesigned and optimized for you
- Most cited—more than 40K scientific publications
- Higher sensitivity and precision with MGB probe chemistry
TaqMan Assay performance guarantee
If a TaqMan Assay doesn't perform as described on our website or datasheet, we'll replace the product at no cost to you or provide you with a credit for a future purchase.
Duplexing with TaqMan Assays
Duplexing in qPCR is the simultaneous detection of two target sequences in a single reaction. Its key benefits come from minimizing the impact of pipetting errors and maximizing experimental efficiency.
Endogenous control assays
Pre-designed TaqMan probe and primer sets enable you to normalize the amount of sample RNA or DNA in a reaction. Controls are available for the most common human, mouse, rat, and eukaryotic genes.
Mutation detection assays
TaqMan Mutation Detection Assays are powered by allele-specific castPCR technology to detect and measure somatic mutations in genes. They are compatible with different sample types, such as cell lines, FFPE tissue samples, and fresh-frozen tissue samples.
Non-coding RNA assays
TaqMan Non-coding RNA Assays provide a highly reliable way to measure expression of long non-coding transcripts. These assays help provide the high specificity and sensitivity you need for quantification and validation studies.
If your research application requires special assay manufacturing modifications, such as a different dye or assay volume, or you would like to have your assays plated in a specific way, our custom services can help.
Protein expression assays
TaqMan Protein Assays combine the sensitive, specific protein-binding capabilities of antibodies with the relative quantitation capabilities of 5' nuclease real-time PCR.
Power to the pros
Work smarter with lab-changing qPCR innovations you’ve only imagined
- Meet the QuantStudio 6 & 7 Pro real-time PCR systems ›
- Discover time-saving TaqMan Array plates with RFID ›
- Complement your qPCR workflow with optimized master mixes, reagents, and kits ›
Taq Talk video series
Episode 1: Understanding real-time PCR terminology
Episode 2: Phases of real-time PCR and why they’re important
Episode 3: What are Ct values in real-time PCR?
Episode 4: How TaqMan real-time PCR assays work
Learning and support
Real-time PCR basics
Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for real-time PCR, we have the learning material, including videos and webinars, to help you understand the technology and get started quickly.
Real-time PCR Support Center
One resource for all your real-time PCR support needs. Navigate through the qPCR application areas to obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday qPCR problems.
For Research Use Only. Not for use in diagnostic procedures.