Applied Biosystems predesigned TaqMan Assays consist of target-specific primers and one or more probes optimized for specific applications, including gene expression, SNP genotyping, miRNA, mutation detection, and copy number variation analysis.
For predesigned assays:Launch assay search wizard
For custom solutions:Launch custom design tool
Applied Biosystems TaqMan Assays are the industry-leading choice for 5' nuclease qPCR assays. They are designed using a highly sophisticated oligonucleotide probe/primer design pipeline including robust primer design algorithms and an extensive array of bioinformatics tools and processes to automate assay design. There are more than 20 million predesigned assays to choose from—all backed by a performance guarantee.
For our standard search experience, use the search bar below. If you are new to TaqMan Assays or just want step-by-step guidance, use the TaqMan Assay Search Wizard.
To start your search, enter keywords such as gene symbol or name, RefSeq number, disease, pathway, or TaqMan Assay ID number. Choose application and species to further refine your search.
Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. No additional design, optimization, or melt curve analysis needed. Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves—not to mention they’re backed by our TaqMan Assay performance guarantee. You can also order your assay with special off-catalog manufacturing modifications using our Specialty service or leverage Thermo Fisher’s manufacturing infrastructure with our GMP oligos optionsor OEM and contract manufacturing services.
Can’t find what you need from our portfolio of predesigned assays? Use our TaqMan Custom Design Assay Tool to easily design your own assay based on your target sequence of interest or submit the primer/probe sequences of your own design. Each custom assay is a mix of forward primer, reverse primer, and FAM and/or VIC dye-labeled TaqMan MGB probe.
Design your own TaqMan primer and probe sets from your choice of dye-labeled TaqMan MGB, TAMRA, or QSY custom probes and Applied Biosystems unlabeled, sequence detection primers.
TaqMan Array cards & plates
Individual tubes or tube sets available in XS, S, M, L
96- and 384-well plates with pre-spotted assays
384-well microfluidic cards with pre-spotted assays
3,072 through-holes with pre-spotted assays
High flexibility for low throughput studies
Supports automation for medium-to-high throughput projects
Easy and convenient–no robotics needed
Higher throughput for routine applications or larger projects
Available for both predesigned and custom designed assays.
Offered in a variety of content options:
Available in off-the-shelf, inventoried arrays as well as made-to-order with total flexibility using predesigned and/or custom assays.
Looking to commercialize? Contact our OEM services team today ›
If a TaqMan Assay doesn't perform as described on our website or datasheet, we'll replace the product at no cost to you or provide you with a credit for a future purchase.
Duplexing in qPCR is the simultaneous detection of two target sequences in a single reaction. Its key benefits come from minimizing the impact of pipetting errors and maximizing experimental efficiency.
Pre-designed TaqMan probe and primer sets enable you to normalize the amount of sample RNA or DNA in a reaction. Controls are available for the most common human, mouse, rat, and eukaryotic genes.
TaqMan Mutation Detection Assays are powered by allele-specific castPCR technology to detect and measure somatic mutations in genes. They are compatible with different sample types, such as cell lines, FFPE tissue samples, and fresh-frozen tissue samples.
TaqMan Non-coding RNA Assays provide a highly reliable way to measure expression of long non-coding transcripts. These assays help provide the high specificity and sensitivity you need for quantification and validation studies.
If your research application requires special assay manufacturing modifications, such as a different dye or assay volume, or you would like to have your assays plated in a specific way, our custom services can help.
TaqMan Protein Assays combine the sensitive, specific protein-binding capabilities of antibodies with the relative quantitation capabilities of 5' nuclease real-time PCR.
Explore the full breadth of our Applied Biosystems real-time PCR offering ›
Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for real-time PCR, we have the learning material, including videos and webinars, to help you understand the technology and get started quickly.
One resource for all your real-time PCR support needs. Navigate through the qPCR application areas to obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday qPCR problems.
Access the files you need for your TaqMan assay, array card, and array plate products.
For Research Use Only. Not for use in diagnostic procedures.