TaqMan Real-time PCR Assays

Applied Biosystems TaqMan real-time PCR assays consist of target-specific primers and one or more probes optimized for specific applications, including gene expression, SNP genotyping, miRNA, mutation detection, and copy number variation analysis.

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TaqMan Assays for every qPCR application

 


Quantitative PCR (qPCR) or real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, supported by intuitive data analysis software, to help harness the power of qPCR across a rich and diverse set of applications.

TaqMan Real-time PCR Assays for Gene Expression, Genotyping, microRNA analysis and other qPCR applications. Available formats include tubes, array cards and plates, microarray or component-level primers and probes.

Gene expression analysis

  • Detect virtually any gene product with our predesigned assays
  • Best-coverage assays to detect highest number of transcript variants possible

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MicroRNA analysis

  • Detect targets with as few as 60 copies of input microRNA (miRNA)
  • Compatible with tissue and biofluids including serum and plasma

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Genotyping

  • Largest selection of predesigned assays for genotyping every SNP of interest as well as polymorphisms associated with drug metabolism (DME)

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qPCR assay and array products

 

 

Assays exactly as you need them:

Predesigned TaqMan Assays

Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. No additional design, optimization, or melt curve analysis needed. Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves—not to mention they’re backed by a performance guarantee.

We currently offer more than 20 million predesigned TaqMan Assays to address nearly every qPCR application:

2.8 million assays
32   species

1.8 million assays

17 million assays

Custom assay design

Can’t find what you need from our portfolio of predesigned assays? Use our TaqMan Custom Design Assay Tool to easily design your own assay based on your target sequence of interest or submit the primer/probe sequences of your own design. Each custom assay is a mix of forward primer, reverse primer, and FAM and/or VIC dye-labeled TaqMan MGB probe.

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Primers & probes

Design your own TaqMan primer and probe sets from your choice of dye-labeled TaqMan MGB, TAMRA, or QSY custom probes and Applied Biosystems unlabeled, sequence detection primers.

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Tubes

TaqMan Array Cards & Plates

OpenArray

Individual tubes or tube sets available in XS, S, M, L

96- and 384-well plates with pre-spotted assays

384-well microfluidic cards with pre-spotted assays

3,072 through-holes with pre-spotted assays

High flexibility for low throughput studies

Supports automation for medium-to-high throughput projects

Easy and convenient–no robotics needed

Higher throughput for routine applications or larger projects

Available for both predesigned and custom designed assays.

Offered in a variety of content options:

  • Preconfigured panels using the most relevant TaqMan GEx Assays for your target–inventoried and ready to ship
  • Made-to-order, Flexible TaqMan panels–start with a curated list of assays, then modify to fit your requirements
  • Custom TaqMan Arrays–Configure from scratch using any combination of TaqMan Assays

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Available in off-the-shelf, inventoried arrays as well as made-to-order with total flexibility using predesigned and/or custom assays.

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About
TaqMan Assays

 

 

Applied Biosystems TaqMan Assays are the industry-leading choice for 5' nuclease qPCR assays. They are designed using a highly sophisticated oligonucleotide probe/primer design pipeline.

 

This includes robust primer design algorithms and an extensive array of bioinformatics tools and processes to automate assay design.

Why choose TaqMan Assays?

  • Easy-to-use—more than 20 million assays predesigned and optimized for you
  • Most cited—more than 40K scientific publications
  • Higher sensitivity and precision with MGB probe chemistry

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TaqMan Assay performance guarantee

If a TaqMan Assay doesn't perform as described on our website or datasheet, we'll replace the product at no cost to you or provide you with a credit for a future purchase.

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Additional qPCR applications & products

 

 

  • Duplexing with TaqMan Assays

    Duplexing in qPCR is the simultaneous detection of two target sequences in a single reaction. Its key benefits come from minimizing the impact of pipetting errors and maximizing experimental efficiency.

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  • Endogenous control assays

    Pre-designed TaqMan probe and primer sets enable you to normalize the amount of sample RNA or DNA in a reaction. Controls are available for the most common human, mouse, rat, and eukaryotic genes.

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  • Mutation detection assays

    TaqMan Mutation Detection Assays are powered by allele-specific castPCR technology to detect and measure somatic mutations in genes. They are compatible with different sample types, such as cell lines, FFPE tissue samples, and fresh-frozen tissue samples.

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  • Non-coding RNA assays

    TaqMan Non-coding RNA Assays provide a highly reliable way to measure expression of long non-coding transcripts. These assays help provide the high specificity and sensitivity you need for quantification and validation studies.

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  • Specialty manufacturing

    If your research application requires special assay manufacturing modifications, such as a different dye or assay volume, or you would like to have your assays plated in a specific way, our custom services can help.

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  • Protein expression assays

    TaqMan Protein Assays combine the sensitive, specific protein-binding capabilities of antibodies with the relative quantitation capabilities of 5' nuclease real-time PCR.

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Featured videos

 

 

 

 

Learning and support

 

 

Real-time PCR basics

Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for real-time PCR, we have the learning material, including videos and webinars, to help you understand the technology and get started quickly.

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Real-time PCR Support Center

One resource for all your real-time PCR support needs. Navigate through the qPCR application areas to obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday qPCR problems.

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TaqMan documentation files

Access the files you need for your TaqMan assay, array card, and array plate products.

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