This learning center is a centralized location for the presentation of educational resources across a wide array of real-time PCR application areas.  Select an application area below to view a collection of many different types of learning resources on that subject, including articles, application notes, videos, and webinars.  Visit the Resource Library to see all real-time PCR resources presented together in a searchable, sortable table.

Real-time PCR subtopics

Real-time PCR Basics

Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for qPCR, we have the learning material, including videos and webinars, to help you understand the technology and get started quickly.

Gene Expression

Gene expression profiling simultaneously compares the expression levels of multiple genes between two or more samples. This analysis can help scientists identify the molecular basis of phenotypic differences and select gene expression targets for in-depth study. Gene expression profiling provides valuable insight into the role of differential gene expression in normal biological and disease processes.

Genotyping

Scientists employing genotyping analysis are looking to untangle the complex relationships between genotype and phenotype in studies focusing on single nucleotide polymorphisms (SNPs), insertion or deletion polymorphisms (indels), and copy number variants (CNVs).

miRNA Analysis

The high relative stability of miRNA in biofluids and the ability of miRNA expression profiles to accurately classify discrete tissue types and disease states have positioned miRNA quantification as a promising new tool for a wide range of biomarker discoveries. MicroRNAs are gaining popularity in emerging applications such as early detection of cancer, cardiovascular diseases, and pathway analysis.