The gene-to-protein advantage
Expedite your protein and antibody workflows from discovery to commercialization with our complete gene-to-protein workflow. By partnering with us, we can help speed your discovery process toward rapid expression and purification of your desired protein from transiently transfected mammalian cells. Using a combination of gene optimization, synthetic DNA expertise, and advanced Gibco expression systems, protein yields can increase by as much as 10x.
We’re here to support all or part of your protein expression and purification needs—from gene synthesis and optimization to early production pilot studies, all the way up to 25 L production runs. Move from idea to purified and documented protein in as few as 5 weeks with GeneArt gene-to-protein services.
How to request a quote for a project
For a custom quote please use our online request form and we will respond with a price and production time. You can also use this form to conveniently re-order a previous GeneArt project.
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Higher protein yields enabled by sequence optimization, DNA synthesis expertise, and Gibco expression systems
Fast, reliable, and high-yield expression of recombinant proteins in mammalian and insect lines is essential for many aspects of biomedical research and drug development. Low expression yields are a serious problem and often limit subsequent structural and functional analyses.
Our solution is to harness the power of gene optimization with our synthetic gene expertise and advanced Gibco expression systems to provide maximum protein yields of correctly folded protein (Figure 1). The GeneArt antibody production service begins with de novo gene synthesis, using a proprietary Invitrogen GeneArt GeneOptimizer algorithm to optimize recombinant protein expression prior to synthesis.
By starting protein expression projects with gene synthesis, this provides a fast and efficient way to screen for error-free clones to quickly identify the most promising candidates for further scale‑up production.
Advantages of using Gibco Expi293 or ExpiCHO systems
- Available in both mammalian (HEK293, CHO-S) and insect (Sf9) cell line formats
- Expression yields of up to 3 g/L with substantial more protein in less volume
- Scalable expression from <1 mL to >10 mL
- Turnaround time of 5–14 days for rapid protein production
- We also offer Expi293F Cells (cGMP banked) and ExpiCHO-S Cells (cGMP banked)
Visit our Cell Biology Services page to learn more about our Gibco Expi transient protein expression systems and additional services.
We also offer insect expression via our Gibco ExpiSf Expression system.
- Highly flexible and extensible
- Small-scale 24-/96-multiwell expression
- 30 mL to >25 L production scales
High-throughput (HTP) small-scale ExpiCHO Expression and purification
GeneArt HTP protein expression ExpiCHO services offer small scale options to rapidly analyze variant constructs to accelerate antibody discovery. This reliable and cost-effective expression and purification service provides the ability to effectively accelerate discovery and upstream development processes. We offer 24-well and a 96-well formats, and ship both protein and DNA from your initial starting sequence in as few as 21 days.
|Size||Culture Volume||Samples per plate||Controls per plate||Quantity after purification||Final Volume||Control Minimum|
|24-well||2.5 mL||20||4||10 g–1.8 mg||350 ul||200 ug|
|96-well||0.8 mL||92||4||1–500 ug||150 ul||75 ug|
Controls are included on each place across the plate (+ rIgG, - mock). Mock wells can be replaced by customer specific control (i.e. GM control)
- Highly flexible—capacity dependent on product mix
- >5,000 proteins produced per year (various scales)
- Multiple ÄKTA Pure, Purifier, and Xpress devices
- Up to ≥300 antibody purifications/week at <250 mL scale
- Up to ≥60 antibody purifications/week at 1 L scale
- Up to ≥10 antibody purifications/month at 25 L scale
- ÄKTA Flux system for diafiltration
- Multiple tangential flow filtration devices
Our purification methods, tags, and options are summarized in the table below.
|Service project phase||Standard specifications|
Frequently asked questions (FAQ)
GeneArt Gene-to-Protein Services offer a fee-for-service protein services that specializes in research use only (RUO) transient protein expression and purification. We excel in early stage R&D, discovery, and mammalian expression and purification services. We offer protein services as a complement to our gene synthesis services, which leverage the proprietary Invitrogen GeneOptimizer tool to increase protein yields 3–10x vs. wild type sequences. Although GeneArt service does not offer stable cell line development or CDMO/CMO scale-up services, Thermo Fisher Scientific does offer such services. Additionally, unlike a CRO, GeneArt Services does not provide scientific or technical advisory support services. This allows us to produce and purify proteins as quickly as possible and provides cost-saving benefits for our customers. In certain circumstances, we will execute CRO-like services for strategic partnerships and we’ll be happy to explore unique partnership models upon request. Contact firstname.lastname@example.org for any questions or additional information.
The cells we use are adapted for growth in suspension. For custom projects, we can use adherent cells for protein expression for an additional fee.
We use shaker cultures and WAVE™ bag technology. Currently, 25 L is our maximum culture volume.
Currently, we prioritize intracellular and secreted proteins (e.g., antibodies, cytokines), and secretable proteins with an artificial secretion leader. On occasion and upon approval, we will explore membrane protein projects with certain partnership requirements. Please contact us at email@example.com for more information.
GeneArt gene-to-protein services are a fee-for-service organization. This means the customer is paying for the work, rather than the end product (unless otherwise specified in project setup and the final quote). With more than 20 years of experience in protein expression, we are confident in our capabilities, but we understand unique situations arise. If you are a current customer, please contact firstname.lastname@example.org for more information. Not all inquiries will result in a partial or full refund, but we will do our best to satisfy our customers.
No, the prices are only for the protein services; gene synthesis, subcloning, and plasmid preparation work are not included and will be listed on the quote separately.
We recommend that you let GeneArt protein services produce an expression-optimized gene subcloned into a vector of your choice for the production of your protein. In limited occasions, we can produce protein from your construct, as long as you first test it for expression and send us the results of your analysis (e.g., estimated protein quantification with Coomassie staining) and information on the cell culture volume you used. Please contact us at email@example.com for more information.
In order to provide a firm price estimate, we require information on the productive performance of your protein. That is the purpose of the GeneArt gene-to-protein production pilot, where we evaluate expression and purification performance of your construct. Once the pilot is complete, we can provide you with a binding quote for a specific protein amount.
Unfortunately, we do not. Cell line and protein production projects have a significant number of variables and processes, thus each project is priced individually. Please define a specific project scope in an email to firstname.lastname@example.org and we will get back to you with a price quote.
We use all available methods for purification. This methods include affinity-tag, Protein G/A (for antibodies or their derivatives), or combinations of ion exchange/hydrophobic interaction chromatography and gel filtration chromatography.
Yes, we use tags that can be cleaved off after purification and removed from the purified protein (for example, by introducing a thrombin or TEV protease cleavage sequence during gene synthesis—some amino acids may be left on the protein after cleavage).
Can’t find the answer you need here?
Please email your question to email@example.com and we will personally answer your question.
For Research Use Only. Not for use in diagnostic procedures.