Alexa Fluor Secondary Antibodies

Browse our comprehensive portfolio of Invitrogen Alexa Fluor and Alexa Fluor Plus secondary antibodies for fluorescent and chemiluminescent detection of primary antibodies in a wide range of applications, such as cell imaging, flow cytometry and western blotting.

Invitrogen Alexa Fluor protein and antibody conjugates produce fluorescence outputs that exceed those of other spectrally similar fluorophore conjugates, and do not photobleach as easily as classic fluorescent secondary antibodies. With more than 30,000 publications, the Alexa Fluor dye family has been successfully used over the last three decades of fluorescence imaging.

Alexa Fluor Plus Secondary Antibodies

Need to detect and visualize low-abundance targets in rare or precious samples?

Alexa Fluor Plus Secondary Antibodies

Compare to traditional Alexa Fluor secondary antibodies:

  • Higher signal-to-noise ratio
  • Lower cross-reactivity

Learn more

Alexa Fluor Secondary Antibody Product Information

Use Designed for use in a variety of cell analysis and protein analysis applications including imaging and fluorescent western blotting.
Applications Cell Imaging Western blotting (WB)
Product attributes

Cell Imaging

  • Variety of host and target specificities available

Multiplex, fluorescent western blotting

  • Broad range of conjugates

Targets available

  • 30 host and target specificities
  • Below is a list of the specificities that we offer Alexa Fluor Secondary antibodies for:
  • Chicken
  • Goat
  • Guinea pig
  • Hamster
  • Human
  • Rabbit
  • Rat
  • Sheep
  • Multiple isotype options available for some host and target specificities
Fluorophores available
  • 18 Alexa Fluor dyes
  • Click links below to see list of available products per fluorophore
Mountant compatibility
  • Most standard mountants

Recommended:

Fixative compatibility

  • Most standard fixatives
Recommended:
Organelle staining compatibility
  • Most standard organelle-specific antibodies and stains
Recommended:

Alexa Fluor ReadyProbes Secondary Antibodies

ReadyProbes secondary antibodies are conjugated with bright and photostable Alexa Fluor 488 and Alexa Fluor 594 dyes to offer convenient and robust labeling. These Alexa Fluor secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes reagents, these secondary antibodies are provided in a convenient dropper bottle format. No pipetting or dilutions are required. Just add 2 drops per milliliter of buffer to stain your cells, and follow the icon-based protocol.

CAKI cell peroxisomes

CAKI cell peroxisomes labeled using a rabbit anti-PMP70 antibody, followed by detection with Alexa Fluor 488 Goat Anti–Rabbit IgG Antibody ReadyProbes Reagent.

Alexa Fluor ReadyProbes Secondary Antibody products

 
Alexa Fluor 488
Alexa Fluor 594
Common filter
FITC
Texas Red
Ex/Em (nm)
495/518
590/617
Donkey anti-mouse IgG antibody conjugate
Goat anti-rabbit IgG antibody conjugate
Donkey anti-rabbit IgG antibody conjugate
Goat anti-mouse IgG antibody conjugate

See more ReadyProbes Ready-to-Use Imaging Reagents

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