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Figure 1. Spectral signature of Alexa Fluor 660.
Alexa Fluor 660 has a unique signature optimized for flow cytometers with spectral capabilities. This fluorophore can be used for cell surface, intracellular, and intranuclear antigens. It should be used in complex multicolor panels to fit between fluorophores Alexa Fluor 647 and Alexa Fluor 700.
![]() Initial brightness | The red laser–excitable Alexa Fluor 660 dye is optimized for use in spectral flow cytometry applications. | ||
![]() Photostability in buffer |
633/640 | 662 | 690 | ||
![]() Laser line | ![]() Excitation max |
We offer Alexa Fluor 660 dye conjugated to primary antibodies for use in flow cytometry.
Learn more here:
Figure 2. CD4-Alexa Fluor 660 example. C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody, Alexa Fluor 700 (Cat. No. 56-0033-82), CD19 Monoclonal Antibody, APC (Cat. No. 17-0193-82), and CD25 Monoclonal Antibody, Alexa Fluor 647 (Cat. No. 51-0251-82) and 0.125 µg of CD4 Monoclonal Antibody, Alexa Fluor 660. Cells in the lymphocyte gate were used for analysis and gated on CD3 positive/CD19 negative cells (left). CD3 positive cells were further plotted as CD4 versus CD25 to determine expression of CD25 (right). This data was collected on a 5-laser Cytek Aurora full spectral cytometer. As shown here, Alexa Fluor 660 is compatible with Alexa Fluor 647 and Alexa Fluor 700 when proper panel design principles are applied.
Figure 3. Foxp3-Alexa Fluor 660 staining. BALB/c mouse splenocytes were surface-stained with CD25 Monoclonal Antibody, Alexa Fluor 488 (Cat. No. 53-0251-82). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) and protocol, with 0.25 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 660 (Cat. No. 606-4321-81) (left) or 0.25 µg of FOXP3 Monoclonal Antibody, Alexa Fluor 660 (right). Cells in the lymphocyte gate were used for analysis. This data was collected on a 5-laser Cytek Aurora full spectral cytometer.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.