Brilliant Ultra Violet™ dyes, including Brilliant Ultra Violet™ 496 (BUV496), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors allowing for larger panels.
BUV496 is a tandem dye excited by the 355 nm UV laser and emits at 496 nm. This dye has a low-to-medium relative brightness and can be used for cell surface, intracellular, and nuclear staining.
When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend that you use Super Bright Complete Staining Buffer (Cat. No. SB-4401-42) or Brilliant Stain Buffer (Cat. No. 00-4409-42) to minimize any non- specific polymer interactions.
|The UV laser-excitable BUV496 dye is optimized for use both cell surface and intracellular flow applications. Can be used for spectral flow cytometry applications.|
Photostability in buffer
We offer BUV496 dye conjugated to primary antibodies for use in flow cytometry.
Figure 1. Excitation and emission of Brilliant Ultra Violet™ 496 dye (BUV496).
Figure 2. Spectral signature of BUV496 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with clone SK7 (hCD3) conjugated to BUV496 dye (Cat. No. 364-0036-42) were used for analysis.
Figure 3. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (Cat. No. 16-0031-85) (right). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) and protocol, with CD45R (B220) Monoclonal Antibody, FITC (Cat. No. 11-0452-82) and 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet™ 496 dye (Cat. No. 364-5698-82). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Cat. No. L34962).
Figure 4. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC (Cat. No. 11-0031-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet™ 496 dye (Cat. No. 364-4321-81) (left) or 0.25 µg of CD45R (B220) Monoclonal Antibody, Brilliant Ultra Violet™ 496 dye (Cat. No. 364-0452-82) (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50).
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.