apc

Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.

In practical applications, the sensitivity of APC conjugates is usually 5 to 10 times greater than that of the corresponding fluorescein conjugate. In addition, RPE exhibits:

  • Long-wavelength fluorescence emission
  • Large Stokes shifts
  • Quench-resistance due to the protein backbone
  • Very high water solubility
  • Homogeneous structure with defined molecular weights

We offer APC conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. In addition, tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength.

Allophycocyanin (APC) dashboard


Initial brightness

 
 
 
 
 
Allophycocyanin, also known as APC, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability.  
   

Photostability in buffer

 
 
 
 
 

  200 594–633 Cy5 650 660  
 
Stain index

Laser line

Common filter set

Excitation max

Emission max
 

Allophycocyanin (APC) products

We offer a broad range of APC products, including primary antibodies, secondary antibodies, streptavidin conjugates, and more.

apc-data

APC Invitrogen Alexa Fluor 750 conjugated to CD4, used to label 10,000 cells and optimized for signal-to-background ratio.

 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.