Invitrogen Alexa Fluor 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 680 dye is pH-insensitive over a wide molar range.
The fluorescence quantum yield and high photostability in antifade allow detection of low-abundance biological structures in fixed cells with great sensitivity. Alexa Fluor 680 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.
We offer Alexa Fluor 680 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
|Near IR dye provides signal in a spectral range easily separated from tissue autofluorescence. Useful for multiplexing western blot detection. In addition, useful in stochastic optical reconstruction microscopy (STORM) where it is used a reporter for dSTORM.|
Photostability in buffer
Photostability in antifade
Performance in SRM
We offer a broad range of Alexa Fluor 680 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more:
Golgi in fixed and permeabilized NIH 3T3 mouse fibroblast cells was labeled with green-fluorescent Invitrogen Alexa Fluor 488 conjugate of HPA lectin. Mitochondria were labeled with a primary antibody directed against OxPhos Complex V Inhibitor Protein and visualized using far-red-fluorescent Alexa Fluor 680 goat anti-mouse IgG. Filamentous actin was stained with red-orange–fluorescent Invitrogen Alexa Fluor 568 phalloidin. Nuclei were stained with blue-fluorescent DAPI.
Select dye-labeling chemistries for conjugation with the following reactive groups:
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon Alexa Fluor 680 Mouse IgG1 Labeling Kit||Z25010|
|<1–20 µg mouse IgG2a||Zenon Alexa Fluor 680 Mouse IgG2a Labeling Kit||Z25110|
|<1–20 µg mouse IgG2b||Zenon Alexa Fluor 680 Mouse IgG2b Labeling Kit||Z25210|
|Conventional antibody/protein labeling|
|100 µg antibody/protein||Alexa Fluor 680 Antibody/Protein Labeling Kit||A20188|
|1 mg antibody/protein||Alexa Fluor 680 Antibody/Protein Labeling Kit||A20172|
|0.5–3 mg antibody||Alexa Fluor SAIVI 680 Antibody Labeling Kit||S30045|
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.