Alexa Fluor® 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor® 680 dye is pH-insensitive over a wide molar range.
The fluorescence quantum yield and high photostability in antifade allow detection of low-abundance biological structures in fixed cells with great sensitivity. Alexa Fluor® 680 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.
We offer Alexa Fluor® 680 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Alexa Fluor® 680 dye dashboard
Photostability in buffer
Photostability in antifade
Performance in SRM
Common filter set
Alexa Fluor® 680 products
We offer a broad range of Alexa Fluor® 680 products including labeling kits, secondary antibodies, bioconjugates, and more:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits for direct detection
- Secondary antibodies for flexible labeling
- Streptavidin conjugates for signal amplification
- Tyramide labeling kits for ultimate sensitivity
- Phalloidin conjugates for actin staining
- Annexin V conjugates for apoptosis imaging
- Labeled nucleotides for hybridization probes
Golgi in fixed and permeabilized NIH 3T3 mouse fibroblast cells was labeled with green-fluorescent Alexa Fluor® 488 conjugate of HPA lectin. Mitochondria were labeled with a primary antibody directed against OxPhos Complex V Inhibitor Protein and visualized using far-red-fluorescent Alexa Fluor® 680 goat anti-mouse IgG. Filamentous actin was stained with red-orange–fluorescent Alexa Fluor® 568 phalloidin. Nuclei were stained with blue-fluorescent DAPI.
Select dye-labeling chemistries for conjugation with the following reactive groups:
Alexa Fluor® 680 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon® Alexa Fluor® 680 Mouse IgG1 Labeling Kit||Z25010|
|<1–20 µg mouse IgG2a||Zenon® Alexa Fluor® 680 Mouse IgG2a Labeling Kit||Z25110|
|<1–20 µg mouse IgG2b||Zenon® Alexa Fluor® 680 Mouse IgG2b Labeling Kit||Z25210|
|Conventional antibody/protein labeling|
|100 µg antibody/protein||Alexa Fluor® 680 Antibody/Protein Labeling Kit||A20188|
|1 mg antibody/protein||Alexa Fluor® 680 Antibody/Protein Labeling Kit||A20172|
|0.5–3 mg antibody||Alexa Fluor® SAIVI™ 680 Antibody Labeling Kit||S30045|
Alexa Fluor® dye family
Illuminating research for over 15 years
Alexa Fluor® dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor® dyes.
- Learn more about the brightness of Alexa Fluor® dyes
- Learn more about the photostability of Alexa Fluor® dyes
- Read all about Alexa Fluor® dyes in the Molecular Probes® Handbook