Alexa Fluor® 680 dyeInvitrogen Alexa Fluor 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 680 dye is pH-insensitive over a wide molar range.

The fluorescence quantum yield and high photostability in antifade allow detection of low-abundance biological structures in fixed cells with great sensitivity. Alexa Fluor 680 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.

We offer Alexa Fluor 680 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 680 dye dashboard


Initial brightness

 
 
 
 
 
Near IR dye provides signal in a spectral range easily separated from tissue autofluorescence. Useful for multiplexing western blot detection. In addition, useful in stochastic optical reconstruction microscopy (STORM) where it is used a reporter for dSTORM. 
  

Photostability in buffer

 
 
 
 
 
  

Photostability in antifade

 
 
 
 
 
  

Performance in SRM

 
 
 
 
 

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Golgi in fixed and permeabilized NIH 3T3 mouse fibroblast cells was labeled with green-fluorescent Invitrogen Alexa Fluor 488 conjugate of HPA lectin. Mitochondria were labeled with a primary antibody directed against OxPhos Complex V Inhibitor Protein and visualized using far-red-fluorescent Alexa Fluor 680 goat anti-mouse IgG. Filamentous actin was stained with red-orange–fluorescent Invitrogen Alexa Fluor 568 phalloidin. Nuclei were stained with blue-fluorescent DAPI.

Alexa Fluor 680 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Alexa Fluor 680 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target proteinKitCat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1Zenon Alexa Fluor 680 Mouse IgG1 Labeling KitZ25010
<1–20 µg mouse IgG2aZenon Alexa Fluor 680 Mouse IgG2a Labeling KitZ25110
<1–20 µg mouse IgG2bZenon Alexa Fluor 680 Mouse IgG2b Labeling KitZ25210
Conventional antibody/protein labeling
100 µg antibody/proteinAlexa Fluor 680 Antibody/Protein Labeling KitA20188
1 mg antibody/proteinAlexa Fluor 680 Antibody/Protein Labeling KitA20172
0.5–3 mg antibodyAlexa Fluor SAIVI 680 Antibody Labeling KitS30045
Alexa Fluor Dye stained cell

Alexa Fluor dye family

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Violet and Brilliant Ultra Violet are trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.