Alexa Fluor® 680 dyeInvitrogen Alexa Fluor 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 680 dye is pH-insensitive over a wide molar range.

The fluorescence quantum yield and high photostability in antifade allow detection of low-abundance biological structures in fixed cells with great sensitivity. Alexa Fluor 680 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.

We offer Alexa Fluor 680 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 680 dye dashboard


Initial brightness

 
 
 
 
 
Near IR dye provides signal in a spectral range easily separated from tissue autofluorescence. Useful for multiplexing western blot detection. In addition, useful in stochastic optical reconstruction microscopy (STORM) where it is used a reporter for dSTORM.  
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 
   

Performance in SRM

 
 
 
 
 

Golgi in fixed and permeabilized NIH 3T3 mouse fibroblast cells was labeled with green-fluorescent Invitrogen Alexa Fluor 488 conjugate of HPA lectin. Mitochondria were labeled with a primary antibody directed against OxPhos Complex V Inhibitor Protein and visualized using far-red-fluorescent Alexa Fluor 680 goat anti-mouse IgG. Filamentous actin was stained with red-orange–fluorescent Invitrogen Alexa Fluor 568 phalloidin. Nuclei were stained with blue-fluorescent DAPI.
Alexa Fluor 680 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine Thiol

Alexa Fluor 680 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein Kit Cat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1 Zenon Alexa Fluor 680 Mouse IgG1 Labeling Kit Z25010
<1–20 µg mouse IgG2a Zenon Alexa Fluor 680 Mouse IgG2a Labeling Kit Z25110
<1–20 µg mouse IgG2b Zenon Alexa Fluor 680 Mouse IgG2b Labeling Kit Z25210
Conventional antibody/protein labeling
100 µg antibody/protein Alexa Fluor 680 Antibody/Protein Labeling Kit A20188
1 mg antibody/protein Alexa Fluor 680 Antibody/Protein Labeling Kit A20172
0.5–3 mg antibody Alexa Fluor SAIVI 680 Antibody Labeling Kit S30045

Alexa Fluor dye family

Illuminating research for over 15 years

Alexa Fluor® dyesInvitrogen Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.

 

Resources

Molecular Probes Handbook

The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

Go to the Handbook online
Order your copy of the Handbook

Antifade reagents

Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

Learn more about SlowFade Antifade reagents
Learn more about ProLong Antifade reagents

Cell stain tool

Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

Go to the cell stain tool

Fluorescence SpectraViewer

Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer