DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
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Description
A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.Figures
Microglial cells in a rat hippocampus cryosection labeled with DiI acetylated low-density lipoprotein.
Peripheral nervous system of a Drosophila melanogaster embryo.
Four-color image of mouse intestine cryosection.
Schistosoma mansoni parasite.
The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment.
A section of mouse intestine stained with a combination of fluorescent stains.
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI.
Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) stained with MitoTracker® Red CMXRos
Mouse fibroblasts that were fixed, permeabilized and then labeled with BODIPY® TR-X phalloidin, DAPI and a rabbit anti–Golgi complex antibody in conjunction with BODIPY® FL goat anti–rabbit IgG antibody.
Mouse intestine cryosection showing basement membranes.
Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI.
Neuronal cells in a 22-hour zebrafish embryo.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 647 phalloidin and DAPI.
Mouse fibroblasts. BODIPY® FL phallacidin, Texas Red® goat anti–mouse IgG (H+L) antibody and DAPI.
Immunofluorescent staining using mouse anti-golgin-97 monoclonal antibody
X-ray crystal structure of DAPI bound to DNA.
Bacillus subtilis sporulation.
Reversible binding by DSB-X™ biotin.
Mouse kidney section. FluoCells® prepared slide #3.
NIH 3T3 cells showing incorporation of BrdU.
Transformed mouse fibroblasts (CRE BAG 2 cells).
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
Human metaphase chromosomes. ULYSIS Nucleic Acid Labeling Kits.
Bull sperm fertilizing a bovine oocyte. MitoTracker Green FM and DAPI.
Mouse fibroblasts. MitoTracker Red CMXRos, BODIPY FL phallacidin and DAPI.
Cyanobacteria. BODIPY® FL, DAPI and CellTracker™ Green BODIPY®.
Human metaphase chromosomes. ULYSIS Oregon Green® and Alexa Fluor® 594 Nucleic Acid Labeling Kits, and DAPI.
HeLa cells. Anti–bovine a-tubulin, Alexa Fluor® 633 goat anti–mouse IgG conjugate, Alexa Fluor® 680 phalloidin and DAPI.
Chromosome spreads from MRC-5 cultured fibroblasts. ChromaTide® biotin-11-dUTP, TSA Kit #22 or TSA Kit #23, and DAPI.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #2.
Peripheral neurons in mouse intestinal cryosections labeled with rabbit anti–synapsin I antibody.
Human metaphase chromosomes. DAPI.
Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain.
Micrococcus luteus and Bacillus cereus.
Sporulating Bacillus subtilis.
NIH 3T3 cells showing incorporation of BrdU.
Capillary endothelial cells and microglia in a rat brain cryosection.
Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain.
Solemya reidi gill filaments.
Section of mouse intestine.
The intermediate filaments in bovine pulmonary artery endothelial cells, localized using an anti-desmin antibody.
NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
Peroxisome labeling using SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit, for fixed cells:
Microtubules of a fixed bovine pulmonary artery endothelial cell. Anti–bovine a-tubulin, Rhodamine Red™-X goat anti–mouse IgG (H+L) conjugate and DAPI.
Bovine pulmonary artery endothelial cells. Alexa Fluor 647 goat anti–mouse IgG conjugate, Alexa Fluor 546 streptavidin conjugate and DAPI.
Chromatin in PtK2 cells. DAPI.
Eupyrene spermatozoa from the pupal testis of the gypsy moth.
Four-panel composite image of mouse fibroblasts.
Mitochondrial DNA labeled with DAPI and colocalized with MitoTracker® Red in a bovine pulmonary artery endothelial cell.
Mouse brain cryosection. NeuroTrace® 500/525 green-fluorescent Nissl stain and DAPI.
Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green 514 goat anti–mouse IgG antibody, MitoTracker Red CMXRos and DAPI.
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain.
Bovine pulmonary artery endothelial cells.
ubcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes.
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate.
Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody
Mouse kidney section. FluoCells® prepared slide #3.
CHSE cells infected with hematopoietic necrosis virus. DAPI and Texas Red®-X goat anti–mouse IgG antibody.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Intrinsic fluorescence in a mouse renal artery cross-section.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Bovine pulmonary artery endothelial cells were incubated with MitoTracker® Red 580.
NIH 3t3 cells.
Bull sperm prelabeled with MitoTracker® Green FM and used for in vitro fertilization of bovine oocytes.
Reversible binding by DSB-X biotin.
FluoCells® prepared slide #1.
Bovine pulmonary artery endothelial cells (BPAEC). FluoCells prepared slide #2.
Bovine pulmonary artery endothelial cells. anti-alpha-tubulin mouse monoclonal 236-10501, Alexa Fluor® 647 goat anti–mouse IgG antibody, Alexa Fluor® 488 streptavidin and DAPI.
Neural somata from mouse brain section. NeuroTrace 500/525 green-fluorescent Nissl stain, DAPI and CellTracker CM-DiI.
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 358⁄461 |
| Product Size: | 10 mg |
| Shipping Condition: | Room Temperature |
