DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
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Description
A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.Figures
Bovine pulmonary artery endothelial cells (BPAEC). FluoCells prepared slide #2.
Section of mouse intestine.
Bovine pulmonary artery endothelial cells. anti-alpha-tubulin mouse monoclonal 236-10501, Alexa Fluor® 647 goat anti–mouse IgG antibody, Alexa Fluor® 488 streptavidin and DAPI.
Mouse brain cryosection. NeuroTrace® 500/525 green-fluorescent Nissl stain and DAPI.
HeLa cells. Anti–bovine a-tubulin, Alexa Fluor® 633 goat anti–mouse IgG conjugate, Alexa Fluor® 680 phalloidin and DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Microtubules of a fixed bovine pulmonary artery endothelial cell. Anti–bovine a-tubulin, Rhodamine Red™-X goat anti–mouse IgG (H+L) conjugate and DAPI.
Capillary endothelial cells and microglia in a rat brain cryosection.
Mouse fibroblasts. BODIPY® FL phallacidin, Texas Red® goat anti–mouse IgG (H+L) antibody and DAPI.
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody
Human metaphase chromosomes. DAPI.
Bovine pulmonary artery endothelial cells. Alexa Fluor 647 goat anti–mouse IgG conjugate, Alexa Fluor 546 streptavidin conjugate and DAPI.
Bull sperm prelabeled with MitoTracker® Green FM and used for in vitro fertilization of bovine oocytes.
Bacillus subtilis sporulation.
X-ray crystal structure of DAPI bound to DNA.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.
Four-color image of mouse intestine cryosection.
Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse
Peripheral neurons in mouse intestinal cryosections labeled with rabbit anti–synapsin I antibody.
Reversible binding by DSB-X™ biotin.
Peripheral nervous system of a Drosophila melanogaster embryo.
Mouse kidney section. FluoCells® prepared slide #3.
Intrinsic fluorescence in a mouse renal artery cross-section.
Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green 514 goat anti–mouse IgG antibody, MitoTracker Red CMXRos and DAPI.
Solemya reidi gill filaments.
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI.
The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment.
ubcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes.
Peroxisome labeling using SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit, for fixed cells:
Chromosome spreads from MRC-5 cultured fibroblasts. ChromaTide® biotin-11-dUTP, TSA Kit #22 or TSA Kit #23, and DAPI.
Reversible binding by DSB-X biotin.
Mouse kidney section. FluoCells® prepared slide #3.
Transformed mouse fibroblasts (CRE BAG 2 cells).
Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 647 phalloidin and DAPI.
Mitochondrial DNA labeled with DAPI and colocalized with MitoTracker® Red in a bovine pulmonary artery endothelial cell.
The intermediate filaments in bovine pulmonary artery endothelial cells, localized using an anti-desmin antibody.
Bovine pulmonary artery endothelial cells were incubated with MitoTracker® Red 580.
Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI.
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #2.
CHSE cells infected with hematopoietic necrosis virus. DAPI and Texas Red®-X goat anti–mouse IgG antibody.
Microglial cells in a rat hippocampus cryosection labeled with DiI acetylated low-density lipoprotein.
NIH 3T3 cells showing incorporation of BrdU.
Sporulating Bacillus subtilis.
NIH 3t3 cells.
Neural somata from mouse brain section. NeuroTrace 500/525 green-fluorescent Nissl stain, DAPI and CellTracker CM-DiI.
Human metaphase chromosomes. ULYSIS Oregon Green® and Alexa Fluor® 594 Nucleic Acid Labeling Kits, and DAPI.
NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
Chromatin in PtK2 cells. DAPI.
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain.
Cyanobacteria. BODIPY® FL, DAPI and CellTracker™ Green BODIPY®.
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
Bull sperm fertilizing a bovine oocyte. MitoTracker Green FM and DAPI.
Mouse fibroblasts that were fixed, permeabilized and then labeled with BODIPY® TR-X phalloidin, DAPI and a rabbit anti–Golgi complex antibody in conjunction with BODIPY® FL goat anti–rabbit IgG antibody.
Mouse fibroblasts. MitoTracker Red CMXRos, BODIPY FL phallacidin and DAPI.
Micrococcus luteus and Bacillus cereus.
Mouse intestine cryosection showing basement membranes.
Neuronal cells in a 22-hour zebrafish embryo.
Four-panel composite image of mouse fibroblasts.
Immunofluorescent staining using mouse anti-golgin-97 monoclonal antibody
Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) stained with MitoTracker® Red CMXRos
Schistosoma mansoni parasite.
Eupyrene spermatozoa from the pupal testis of the gypsy moth.
NIH 3T3 cells showing incorporation of BrdU.
A section of mouse intestine stained with a combination of fluorescent stains.
Human metaphase chromosomes. ULYSIS Nucleic Acid Labeling Kits.
Bovine pulmonary artery endothelial cells.
FluoCells® prepared slide #1.
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 358⁄461 |
| Product Size: | 10 mg |
| Shipping Condition: | Room Temperature |
