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DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
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Description
A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.Figures
Chromatin in PtK2 cells. DAPI.
Transformed mouse fibroblasts (CRE BAG 2 cells).
Mitochondrial DNA labeled with DAPI and colocalized with MitoTracker® Red in a bovine pulmonary artery endothelial cell.
The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment.
Human metaphase chromosomes. DAPI.
Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green 514 goat anti–mouse IgG antibody, MitoTracker Red CMXRos and DAPI.
Mouse brain cryosection. NeuroTrace® 500/525 green-fluorescent Nissl stain and DAPI.
Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI.
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
X-ray crystal structure of DAPI bound to DNA.
ubcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes.
Intrinsic fluorescence in a mouse renal artery cross-section.
Human metaphase chromosomes. ULYSIS Nucleic Acid Labeling Kits.
NIH 3T3 cells showing incorporation of BrdU.
Bovine pulmonary artery endothelial cells were incubated with MitoTracker® Red 580.
Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain.
Four-color image of mouse intestine cryosection.
NIH 3T3 cells showing incorporation of BrdU.
Bull sperm fertilizing a bovine oocyte. MitoTracker Green FM and DAPI.
Human metaphase chromosomes. ULYSIS Oregon Green® and Alexa Fluor® 594 Nucleic Acid Labeling Kits, and DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Eupyrene spermatozoa from the pupal testis of the gypsy moth.
Sporulating Bacillus subtilis.
Peripheral nervous system of a Drosophila melanogaster embryo.
Neuronal cells in a 22-hour zebrafish embryo.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.
Solemya reidi gill filaments.
Immunofluorescent staining using mouse anti-golgin-97 monoclonal antibody
Capillary endothelial cells and microglia in a rat brain cryosection.
NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
Mouse fibroblasts that were fixed, permeabilized and then labeled with BODIPY® TR-X phalloidin, DAPI and a rabbit anti–Golgi complex antibody in conjunction with BODIPY® FL goat anti–rabbit IgG antibody.
Mouse fibroblasts. MitoTracker Red CMXRos, BODIPY FL phallacidin and DAPI.
Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain.
Neural somata from mouse brain section. NeuroTrace 500/525 green-fluorescent Nissl stain, DAPI and CellTracker CM-DiI.
Microglial cells in a rat hippocampus cryosection labeled with DiI acetylated low-density lipoprotein.
Schistosoma mansoni parasite.
Bovine pulmonary artery endothelial cells.
Bacillus subtilis sporulation.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #2.
Bovine pulmonary artery endothelial cells. Alexa Fluor 647 goat anti–mouse IgG conjugate, Alexa Fluor 546 streptavidin conjugate and DAPI.
Peroxisome labeling using SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit, for fixed cells:
CHSE cells infected with hematopoietic necrosis virus. DAPI and Texas Red®-X goat anti–mouse IgG antibody.
A section of mouse intestine stained with a combination of fluorescent stains.
Mouse kidney section. FluoCells® prepared slide #3.
Mouse kidney section. FluoCells® prepared slide #3.
Four-panel composite image of mouse fibroblasts.
Reversible binding by DSB-X™ biotin.
Micrococcus luteus and Bacillus cereus.
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI.
Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse
Section of mouse intestine.
Mouse fibroblasts. BODIPY® FL phallacidin, Texas Red® goat anti–mouse IgG (H+L) antibody and DAPI.
Mouse intestine cryosection showing basement membranes.
Bovine pulmonary artery endothelial cells (BPAEC). FluoCells prepared slide #2.
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody
The intermediate filaments in bovine pulmonary artery endothelial cells, localized using an anti-desmin antibody.
Reversible binding by DSB-X biotin.
Peripheral neurons in mouse intestinal cryosections labeled with rabbit anti–synapsin I antibody.
Microtubules of a fixed bovine pulmonary artery endothelial cell. Anti–bovine a-tubulin, Rhodamine Red™-X goat anti–mouse IgG (H+L) conjugate and DAPI.
HeLa cells. Anti–bovine a-tubulin, Alexa Fluor® 633 goat anti–mouse IgG conjugate, Alexa Fluor® 680 phalloidin and DAPI.
Bovine pulmonary artery endothelial cells. anti-alpha-tubulin mouse monoclonal 236-10501, Alexa Fluor® 647 goat anti–mouse IgG antibody, Alexa Fluor® 488 streptavidin and DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) stained with MitoTracker® Red CMXRos
Cyanobacteria. BODIPY® FL, DAPI and CellTracker™ Green BODIPY®.
FluoCells® prepared slide #1.
Chromosome spreads from MRC-5 cultured fibroblasts. ChromaTide® biotin-11-dUTP, TSA Kit #22 or TSA Kit #23, and DAPI.
Bull sperm prelabeled with MitoTracker® Green FM and used for in vitro fertilization of bovine oocytes.
NIH 3t3 cells.
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate.
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 647 phalloidin and DAPI.
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 358⁄461 |
| Product Size: | 10 mg |
| Shipping Condition: | Room Temperature |
