DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

DiI LDL (Cat. No. L3482) bound to the surface and internalized in the gut of the parasite Schistosoma mansoni. The distribution of LDL in the parasite is used to study a putative mechanism by which th

Cells were pulsed with BrdU (B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (A21300) and green-fluorescent Alexa Fluo

Mouse intestine cryosection showing basement membranes labeled with chicken IgY anti-fibronectin antibody and Alexa Fluor® 488 Goat Anti–Chicken IgG antibody (A11039, green). Goblet cells and cryp

A section of mouse intestine stained with a combination of fluorescent stains. Fibronectin, an extracellular matrix adhesion molecule, was labeled using a chicken primary antibody against fibronectin

A mixed population of live and isopropyl alcohol–killed Micrococcus luteus and Bacillus cereus simultaneously stained with DAPI (Cat. No. D1306, D3571, D21490) and SYTOX® Green nucleic a

The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment. The membranes and chromosomes of both the forespore and the larger mother cell are stained with FM® 4-64 (r

Actin filaments of fixed bovine pulmonary artery endothelial cells (Cat. no. BPAEC) labeled with Alexa Fluor® 647 phalloidin (Cat. no. A22287). Nuclei were stained with DAPI (Cat. no. D1306, D3571, D2

Bull sperm prelabeled with MitoTracker Green FM (M7514) and used for in vitro fertilization of bovine oocytes. After fertilization, eggs with bound or incorporated sperm were fixed in 2% formal

Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) were incubated in a solution of MitoTracker Red CMXRos (Cat. No. M7512). The sample was subsequently fixed and t

Mouse fibroblasts that were fixed, permeabilized and then labeled with BODIPY® TR-X phalloidin (Cat. no. B7464), DAPI (Cat. no. D1306, D3571, D21490) and a rabbit anti–Golgi complex antibody (provided

FluoCells® prepared slide #3 (Cat. no. F24630) contains a section of mouse kidney stained with a combination of fluorescent dyes. Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), a green-flu

Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain (Cat. no. S32703). Nuclei were stained with DAPI (Cat. no. D1306, D3571, D21490); the densely stained areas are nu

Neural somata from a mouse brain section labeled with NeuroTrace 500/525 green-fluorescent Nissl stain (N21480). Nuclei are labeled with DAPI (D1306, D3571, D21490). Myelin and other lipophilic areas

Neuronal cells in a 22-hour zebrafish embryo were identified with anti–HuC/HuD mouse monoclonal antibody (Cat. No. A21271) and visualized with red-orange–fluorescent Alexa Fluor® 568 goat anti–mouse I

Human metaphase chromosomes stained with DAPI (Cat. no. D1306, D3571, D21490).

The intermediate filaments in bovine pulmonary artery endothelial cells, localized using our anti-desmin antibody (A21283), which was visualized with the Alexa Fluor 647 goat anti–mouse IgG antibody (

The intermediate filaments in bovine pulmonary artery endothelial cells, localized using our anti-desmin antibody (Cat. no. A21283), which was visualized with the Alexa Fluor® 647 goat anti–mouse IgG

Correlated fluorescence imaging of membrane migration, protein translocation, and chromosome localization during Bacillus subtilis sporulation. Membranes were stained with red-fluorescent FM® 4

Probes for fluorescence in situ hybridization (FISH) created using the ULYSIS Nucleic Acid Labeling Kits. Centromere probes to chromosome 15, chromosome 17 and chromosome 1 were easily labeled

Microtubules of a fixed bovine pulmonary artery endothelial cell labeled with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126), and detected with Rhodamine Red™-X goat anti–mouse IgG (Cat. n

lacZ mRNA in transformed mouse fibroblasts (CRE BAG 2 cells) are hybridized with a singly biotinylated, complementary oligonucleotide. Hybrids were then detected by incubation with a streptavid

Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain (Cat. no. N21480). Nuclei are labeled with DAPI (Cat. no. D1306, D3571, D21490). Myelin and othe

Golgi and actin staining in HeLa cells. Golgi in fixed and permeabilized HeLa cells labeled with anti–golgin-97 monoclonal antibody (Cat. No. A21270) and visualized with green-fluorescent Alexa Fluor®

X-ray crystal structure of DAPI (Cat. no. D1306, D3571, D21490) bound to DNA; the image was derived from data submitted to the Protein Data Bank (number PDB 1D30, www.rcsb.org/pdb/, (Nucleic Acids Res

A mouse brain cryosection stained with the neuron-selective NeuroTrace® 500/525 green-fluorescent Nissl stain (Cat. no. N21480). The nuclei of the non-neuronal cells appear blue after incubation with

FluoCells® prepared slide #1 (Cat. no. F14780) showing bovine pulmonary artery endothelial cells incubated with MitoTracker® Red CMXRos (Cat. no. M7512) to label the mitochondria. After fixation and p

Human metaphase chromosomes hybridized to fluorescent probes from two overlapping microdissection libraries. Probes specific to chromosome regions 1p34–35 and 1p36 were labeled using the ULYSIS Orego

A section of mouse intestine was stained with a combination of fluorescent stains. Fibronectin, an extracellular matrix adhesion molecule, was labeled using a chicken primary antibody against fibronec

Mouse intestine cryosection showing basement membranes labeled with our chicken IgY anti-fibronectin antibody (Cat. No. A21316) and the Alexa Fluor® 488 Goat Anti–Chicken IgG (Cat. No. A11039, green).

Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation an

The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment. The membranes and chromosomes of both the forespore and the larger mother cell are stained with FM 4

Fluorescence in situ hybridization detected by tyramide signal amplification. Chromosome spreads were prepared from the cultured fibroblast cell line MRC-5 and hybridized with a biotinylated a

Reversible binding by DSB-X biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti–a-tubulin antibody (A11126), detected with either biotin-XX

Gill filaments from Solemya reidi—a gutless mollusk inhabiting the highly sulfidic sediments characteristic of pulpmill effluents and sewage outfall sites along the western coast of North

HeLa cells labeled with anti–golgin-97 antibody and detected using Alexa Fluor® 488 Goat Anti–Mouse IgG antibody. Fixed, permeabilized HeLa cells were labeled with anti–golgin-97 monoclonal antibody (

Capillary endothelial cells and microglia in a rat brain cryosection labeled with the green-fluorescent Alexa Fluor® 488 isolectin IB₄ (Cat. No. I21411). Neurons were identified using the N

Multinucleate HeLa cell in metaphase: DAPI, anti–bovine α-tubulin, mouse monoclonal 236-10501, biotin-XX conjugate and Alexa Fluor® 568 streptavidin. A multinucleate HeLa cell in metaphase that

Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Cat. no. N21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with

A bovine pulmonary artery endothelial cell (BPAEC) stained with mouse monoclonal anti–ß-tubulin in conjunction with Oregon Green 514 goat anti–mouse IgG antibody (Cat. no. O6383), MitoTracker Red CMXR

Purified bacteriophage T4 replication proteins that have been electrophoresed on a 12% SDS-polyacrylamide gel and then stained with SYPRO® Orange protein gel stain (Cat. no. S6650, S6651). Gel stainin

FluoCells® prepared slide #3 (Cat. no. F24630) containing a 16 µm cryostat section of mouse kidney stained with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), red-orange–f

The peripheral nervous system of a wild-type (Canton-S) Drosophila melanogaster embryo labeled with the monoclonal 22c10 antibody (which detects a microtubule-associated protein) and subsequent

FluoCells® prepared slide #1 (Cat. no. F14780), consisting of bovine pulmonary artery endothelial cells incubated with MitoTracker® Red CMXRos (Cat. no. M7512) to label the mitochondria. After fixati

Photomicrograph of mouse fibroblasts that have been formaldehyde-fixed, acetone-permeabilized and triple-stained with the F-actin–specific probe BODIPY® FL phallacidin (Cat. no. B607), with mouse mono

Peroxisomes were labeled using an antibody directed at peroxisomal membrane protein 70 (PMP-70) and detected with Alexa Fluor® 488 dye–labeled goat anti–mouse IgG secondary antibody. Mitochondria were

Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) that were incubated in a solution of MitoTracker® Red CMXRos (Cat. no. M7512). The sample was subsequently fixed and th

Cells were pulsed with BrdU (Cat. No. B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (Cat. No. A21300) and green-fluo

Neural somata labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain (Cat. no. N21482). Nuclei in this mouse cerebellum section were counterstained with DAPI (Cat. no. D1306, D3571, D21490). Th

Fixed, permeabilized NIH 3t3 cells were labeled with an Alexa Fluor® 488 conjugate of lectin HPA from Helix pomatia (edible snail) (Cat. No. L11271) and counterstained with DAPI (Cat. No. D1306

Bovine pulmonary artery endothelial cells were incubated with MitoTracker® Red 580 (Cat. no. M22425) to label the mitochondria. After fixation, the cells were counterstained with DAPI (Cat. no. D1306,

Peripheral neurons in mouse intestinal cryosections were labeled with rabbit anti–synapsin I antibody (Cat. No. A6442) and detected using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A1100

FluoCells® prepared slide #3 (Cat. no. F24630) contains a mouse kidney section stained with a combination of fluorescent dyes. Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261) is a green-fluor

Subcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes. Filamentous actin (F-actin) was identified with Alexa Fluor® 633 p

The microtubules of fixed HeLa cells labeled with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126) and visualized using Alexa Fluor® 633 goat anti–mouse IgG (Cat. no. A21050), which is pseud

Intrinsic fluorescence in a mouse renal artery cross-section. Nuceli were stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490).