DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
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Description
A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.Figures
Bovine pulmonary artery endothelial cells. Alexa Fluor 647 goat anti–mouse IgG conjugate, Alexa Fluor 546 streptavidin conjugate and DAPI.
Four-color image of mouse intestine cryosection.
NIH 3T3 cells showing incorporation of BrdU.
Mouse brain cryosection. NeuroTrace® 500/525 green-fluorescent Nissl stain and DAPI.
Neuronal cells in a 22-hour zebrafish embryo.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Human metaphase chromosomes. ULYSIS Nucleic Acid Labeling Kits.
Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) stained with MitoTracker® Red CMXRos
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #2.
Intrinsic fluorescence in a mouse renal artery cross-section.
Bacillus subtilis sporulation.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment.
Chromosome spreads from MRC-5 cultured fibroblasts. ChromaTide® biotin-11-dUTP, TSA Kit #22 or TSA Kit #23, and DAPI.
Mouse fibroblasts. BODIPY® FL phallacidin, Texas Red® goat anti–mouse IgG (H+L) antibody and DAPI.
X-ray crystal structure of DAPI bound to DNA.
The intermediate filaments in bovine pulmonary artery endothelial cells, localized using an anti-desmin antibody.
NIH 3T3 cells showing incorporation of BrdU.
Reversible binding by DSB-X™ biotin.
Microtubules of a fixed bovine pulmonary artery endothelial cell. Anti–bovine a-tubulin, Rhodamine Red™-X goat anti–mouse IgG (H+L) conjugate and DAPI.
Peripheral neurons in mouse intestinal cryosections labeled with rabbit anti–synapsin I antibody.
NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
Mouse kidney section. FluoCells® prepared slide #3.
Bull sperm fertilizing a bovine oocyte. MitoTracker Green FM and DAPI.
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI.
Bovine pulmonary artery endothelial cells. anti-alpha-tubulin mouse monoclonal 236-10501, Alexa Fluor® 647 goat anti–mouse IgG antibody, Alexa Fluor® 488 streptavidin and DAPI.
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody
CHSE cells infected with hematopoietic necrosis virus. DAPI and Texas Red®-X goat anti–mouse IgG antibody.
Peroxisome labeling using SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit, for fixed cells:
Bovine pulmonary artery endothelial cells (BPAEC). FluoCells prepared slide #2.
Human metaphase chromosomes. DAPI.
Bovine pulmonary artery endothelial cells were incubated with MitoTracker® Red 580.
Section of mouse intestine.
Reversible binding by DSB-X biotin.
Peripheral nervous system of a Drosophila melanogaster embryo.
Mouse fibroblasts that were fixed, permeabilized and then labeled with BODIPY® TR-X phalloidin, DAPI and a rabbit anti–Golgi complex antibody in conjunction with BODIPY® FL goat anti–rabbit IgG antibody.
Cyanobacteria. BODIPY® FL, DAPI and CellTracker™ Green BODIPY®.
Micrococcus luteus and Bacillus cereus.
A section of mouse intestine stained with a combination of fluorescent stains.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.
Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green 514 goat anti–mouse IgG antibody, MitoTracker Red CMXRos and DAPI.
Schistosoma mansoni parasite.
Four-panel composite image of mouse fibroblasts.
Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain.
Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI.
Mitochondrial DNA labeled with DAPI and colocalized with MitoTracker® Red in a bovine pulmonary artery endothelial cell.
Neural somata from mouse brain section. NeuroTrace 500/525 green-fluorescent Nissl stain, DAPI and CellTracker CM-DiI.
HeLa cells. Anti–bovine a-tubulin, Alexa Fluor® 633 goat anti–mouse IgG conjugate, Alexa Fluor® 680 phalloidin and DAPI.
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
NIH 3t3 cells.
Solemya reidi gill filaments.
Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain.
Human metaphase chromosomes. ULYSIS Oregon Green® and Alexa Fluor® 594 Nucleic Acid Labeling Kits, and DAPI.
Mouse kidney section. FluoCells® prepared slide #3.
Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse
Bovine pulmonary artery endothelial cells. Alexa Fluor® 647 phalloidin and DAPI.
Capillary endothelial cells and microglia in a rat brain cryosection.
Bull sperm prelabeled with MitoTracker® Green FM and used for in vitro fertilization of bovine oocytes.
Eupyrene spermatozoa from the pupal testis of the gypsy moth.
Immunofluorescent staining using mouse anti-golgin-97 monoclonal antibody
ubcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes.
Mouse intestine cryosection showing basement membranes.
Microglial cells in a rat hippocampus cryosection labeled with DiI acetylated low-density lipoprotein.
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain.
FluoCells® prepared slide #1.
Bovine pulmonary artery endothelial cells.
Transformed mouse fibroblasts (CRE BAG 2 cells).
Mouse fibroblasts. MitoTracker Red CMXRos, BODIPY FL phallacidin and DAPI.
Sporulating Bacillus subtilis.
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate.
Chromatin in PtK2 cells. DAPI.
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 358⁄461 |
| Product Size: | 10 mg |
| Shipping Condition: | Room Temperature |
