DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
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Description
A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.Figures
Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI.
Neuronal cells in a 22-hour zebrafish embryo.
Mouse fibroblasts. MitoTracker Red CMXRos, BODIPY FL phallacidin and DAPI.
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI.
Micrococcus luteus and Bacillus cereus.
Capillary endothelial cells and microglia in a rat brain cryosection.
Reversible binding by DSB-X biotin.
Mouse kidney section. FluoCells® prepared slide #3.
Sporulating Bacillus subtilis.
Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) stained with MitoTracker® Red CMXRos
Bull sperm prelabeled with MitoTracker® Green FM and used for in vitro fertilization of bovine oocytes.
Mitochondrial DNA labeled with DAPI and colocalized with MitoTracker® Red in a bovine pulmonary artery endothelial cell.
Cyanobacteria. BODIPY® FL, DAPI and CellTracker™ Green BODIPY®.
Eupyrene spermatozoa from the pupal testis of the gypsy moth.
Bovine pulmonary artery endothelial cells. Alexa Fluor 647 goat anti–mouse IgG conjugate, Alexa Fluor 546 streptavidin conjugate and DAPI.
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody
Bovine pulmonary artery endothelial cells.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.
HeLa cells. Anti–bovine a-tubulin, Alexa Fluor® 633 goat anti–mouse IgG conjugate, Alexa Fluor® 680 phalloidin and DAPI.
Microtubules of a fixed bovine pulmonary artery endothelial cell. Anti–bovine a-tubulin, Rhodamine Red™-X goat anti–mouse IgG (H+L) conjugate and DAPI.
Bovine pulmonary artery endothelial cells (BPAEC). FluoCells prepared slide #2.
Bull sperm fertilizing a bovine oocyte. MitoTracker Green FM and DAPI.
Mouse fibroblasts. BODIPY® FL phallacidin, Texas Red® goat anti–mouse IgG (H+L) antibody and DAPI.
Four-color image of mouse intestine cryosection.
NIH 3T3 cells showing incorporation of BrdU.
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain.
NIH 3t3 cells.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment.
X-ray crystal structure of DAPI bound to DNA.
Immunofluorescent staining using mouse anti-golgin-97 monoclonal antibody
Human metaphase chromosomes. ULYSIS Nucleic Acid Labeling Kits.
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate.
Chromatin in PtK2 cells. DAPI.
Mouse intestine cryosection showing basement membranes.
Solemya reidi gill filaments.
Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain.
Peripheral neurons in mouse intestinal cryosections labeled with rabbit anti–synapsin I antibody.
Bacillus subtilis sporulation.
CHSE cells infected with hematopoietic necrosis virus. DAPI and Texas Red®-X goat anti–mouse IgG antibody.
Reversible binding by DSB-X™ biotin.
Schistosoma mansoni parasite.
Transformed mouse fibroblasts (CRE BAG 2 cells).
Section of mouse intestine.
Peroxisome labeling using SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit, for fixed cells:
Mouse fibroblasts that were fixed, permeabilized and then labeled with BODIPY® TR-X phalloidin, DAPI and a rabbit anti–Golgi complex antibody in conjunction with BODIPY® FL goat anti–rabbit IgG antibody.
Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain.
Intrinsic fluorescence in a mouse renal artery cross-section.
Chromosome spreads from MRC-5 cultured fibroblasts. ChromaTide® biotin-11-dUTP, TSA Kit #22 or TSA Kit #23, and DAPI.
Mouse brain cryosection. NeuroTrace® 500/525 green-fluorescent Nissl stain and DAPI.
NIH 3T3 cells showing incorporation of BrdU.
Four-panel composite image of mouse fibroblasts.
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse
Peripheral nervous system of a Drosophila melanogaster embryo.
Microglial cells in a rat hippocampus cryosection labeled with DiI acetylated low-density lipoprotein.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
The intermediate filaments in bovine pulmonary artery endothelial cells, localized using an anti-desmin antibody.
Bovine pulmonary artery endothelial cells were incubated with MitoTracker® Red 580.
ubcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 647 phalloidin and DAPI.
Mouse kidney section. FluoCells® prepared slide #3.
Deep tissue imaging with SlowFade Glass mountant
A section of mouse intestine stained with a combination of fluorescent stains.
Human metaphase chromosomes. DAPI.
Improved focal depth with SlowFade Glass mountant
NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green 514 goat anti–mouse IgG antibody, MitoTracker Red CMXRos and DAPI.
Neural somata from mouse brain section. NeuroTrace 500/525 green-fluorescent Nissl stain, DAPI and CellTracker CM-DiI.
Bovine pulmonary artery endothelial cells. anti-alpha-tubulin mouse monoclonal 236-10501, Alexa Fluor® 647 goat anti–mouse IgG antibody, Alexa Fluor® 488 streptavidin and DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #2.
Human metaphase chromosomes. ULYSIS Oregon Green® and Alexa Fluor® 594 Nucleic Acid Labeling Kits, and DAPI.
FluoCells® prepared slide #1.
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 358⁄461 |
| Product Size: | 10 mg |
| Shipping Condition: | Room Temperature |
