DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
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Description
A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.Figures
Sporulating Bacillus subtilis.
Microglial cells in a rat hippocampus cryosection labeled with DiI acetylated low-density lipoprotein.
Mouse brain cryosection. NeuroTrace® 500/525 green-fluorescent Nissl stain and DAPI.
Neural somata from mouse brain section. NeuroTrace 500/525 green-fluorescent Nissl stain, DAPI and CellTracker CM-DiI.
Bacillus subtilis sporulation.
Chromosome spreads from MRC-5 cultured fibroblasts. ChromaTide® biotin-11-dUTP, TSA Kit #22 or TSA Kit #23, and DAPI.
Schistosoma mansoni parasite.
Mouse intestine cryosection showing basement membranes.
Microtubules of a fixed bovine pulmonary artery endothelial cell. Anti–bovine a-tubulin, Rhodamine Red™-X goat anti–mouse IgG (H+L) conjugate and DAPI.
FluoCells® prepared slide #1.
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
Mouse kidney section. FluoCells® prepared slide #3.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.
Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse
Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) stained with MitoTracker® Red CMXRos
Eupyrene spermatozoa from the pupal testis of the gypsy moth.
Human metaphase chromosomes. DAPI.
Four-panel composite image of mouse fibroblasts.
Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #2.
Reversible binding by DSB-X™ biotin.
Cyanobacteria. BODIPY® FL, DAPI and CellTracker™ Green BODIPY®.
Bovine pulmonary artery endothelial cells (BPAEC). FluoCells prepared slide #2.
Chromatin in PtK2 cells. DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
A section of mouse intestine stained with a combination of fluorescent stains.
CHSE cells infected with hematopoietic necrosis virus. DAPI and Texas Red®-X goat anti–mouse IgG antibody.
Bovine pulmonary artery endothelial cells.
The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment.
ubcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes.
Bull sperm fertilizing a bovine oocyte. MitoTracker Green FM and DAPI.
The intermediate filaments in bovine pulmonary artery endothelial cells, localized using an anti-desmin antibody.
NIH 3T3 cells showing incorporation of BrdU.
Bovine pulmonary artery endothelial cells were incubated with MitoTracker® Red 580.
Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain.
Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green 514 goat anti–mouse IgG antibody, MitoTracker Red CMXRos and DAPI.
Reversible binding by DSB-X biotin.
NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
Bull sperm prelabeled with MitoTracker® Green FM and used for in vitro fertilization of bovine oocytes.
Bovine pulmonary artery endothelial cells. anti-alpha-tubulin mouse monoclonal 236-10501, Alexa Fluor® 647 goat anti–mouse IgG antibody, Alexa Fluor® 488 streptavidin and DAPI.
Section of mouse intestine.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 647 phalloidin and DAPI.
Capillary endothelial cells and microglia in a rat brain cryosection.
Mitochondrial DNA labeled with DAPI and colocalized with MitoTracker® Red in a bovine pulmonary artery endothelial cell.
Peripheral nervous system of a Drosophila melanogaster embryo.
NIH 3T3 cells showing incorporation of BrdU.
X-ray crystal structure of DAPI bound to DNA.
Intrinsic fluorescence in a mouse renal artery cross-section.
Immunofluorescent staining using mouse anti-golgin-97 monoclonal antibody
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody
Mouse kidney section. FluoCells® prepared slide #3.
Bovine pulmonary artery endothelial cells. Alexa Fluor 647 goat anti–mouse IgG conjugate, Alexa Fluor 546 streptavidin conjugate and DAPI.
Micrococcus luteus and Bacillus cereus.
Peripheral neurons in mouse intestinal cryosections labeled with rabbit anti–synapsin I antibody.
Neuronal cells in a 22-hour zebrafish embryo.
Transformed mouse fibroblasts (CRE BAG 2 cells).
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate.
Mouse fibroblasts that were fixed, permeabilized and then labeled with BODIPY® TR-X phalloidin, DAPI and a rabbit anti–Golgi complex antibody in conjunction with BODIPY® FL goat anti–rabbit IgG antibody.
Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain.
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain.
Four-color image of mouse intestine cryosection.
Human metaphase chromosomes. ULYSIS Nucleic Acid Labeling Kits.
Mouse fibroblasts. BODIPY® FL phallacidin, Texas Red® goat anti–mouse IgG (H+L) antibody and DAPI.
NIH 3t3 cells.
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI.
Human metaphase chromosomes. ULYSIS Oregon Green® and Alexa Fluor® 594 Nucleic Acid Labeling Kits, and DAPI.
Peroxisome labeling using SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit, for fixed cells:
Mouse fibroblasts. MitoTracker Red CMXRos, BODIPY FL phallacidin and DAPI.
Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #1.
Solemya reidi gill filaments.
HeLa cells. Anti–bovine a-tubulin, Alexa Fluor® 633 goat anti–mouse IgG conjugate, Alexa Fluor® 680 phalloidin and DAPI.
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 358⁄461 |
| Product Size: | 10 mg |
| Shipping Condition: | Room Temperature |
