DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

Cells were pulsed with BrdU (Cat. No. B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (Cat. No. A21300) and green-fluo

Gill filaments from Solemya reidi—a gutless mollusk inhabiting the highly sulfidic sediments characteristic of pulpmill effluents and sewage outfall sites along the western coast of North

Four-panel composite image of mouse fibroblasts that were incubated with MitoTracker Red CMXRos (M7512), and then formaldehyde-fixed, acetone-permeabilized and stained with the F-actin–specific probe,

Four-panel composite image of mouse fibroblasts that were incubated with MitoTracker® Red CMXRos (Cat. no. M7512), and then formaldehyde-fixed, acetone-permeabilized and stained with the F-actin–speci

Reversible binding by DSB-X™ biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126), detected with either

Correlated fluorescence imaging of membrane migration, protein translocation, and chromosome localization during Bacillus subtilis sporulation. Membranes were stained with red-fluorescent FM® 4

Photostability comparison of Alexa Fluor® 488 phalloidin (top panel, Cat. no. A12379) and fluorescein phalloidin (bottom panel, Cat. no. F432). Bovine pulmonary artery endothelial cells were labeled

A bovine pulmonary artery endothelial cell (BPAEC) stained with mouse monoclonal anti–ß-tubulin in conjunction with Oregon Green 514 goat anti–mouse IgG antibody (Cat. no. O6383), MitoTracker Red CMXR

FluoCells® prepared slide #1 (Cat. no. F14780), consisting of bovine pulmonary artery endothelial cells incubated with MitoTracker® Red CMXRos (Cat. no. M7512) to label the mitochondria. After fixati

FluoCells® prepared slide #3 (Cat. no. F24630) contains a section of mouse kidney stained with a combination of fluorescent dyes. Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), a green-flu

Methanol-fixed MRC-5 cells stained with SYTO® RNASelect™ green-fluorescent cell stain (Cat. no. S32703). Nuclei were stained with DAPI (Cat. no. D1306, D3571, D21490); the densely stained areas are nu

The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment. The membranes and chromosomes of both the forespore and the larger mother cell are stained with FM® 4-64 (r

FluoCells® prepared slide #3 (Cat. no. F24630) contains a mouse kidney section stained with a combination of fluorescent dyes. Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261) is a green-fluor

Photomicrograph of mouse fibroblasts that have been formaldehyde-fixed, acetone-permeabilized and triple-stained with the F-actin–specific probe BODIPY® FL phallacidin (Cat. no. B607), with mouse mono

Neural somata from a mouse brain section labeled with NeuroTrace 500/525 green-fluorescent Nissl stain (N21480). Nuclei are labeled with DAPI (D1306, D3571, D21490). Myelin and other lipophilic areas

Neural somata labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain (Cat. no. N21482). Nuclei in this mouse cerebellum section were counterstained with DAPI (Cat. no. D1306, D3571, D21490). Th

Human metaphase chromosomes hybridized to fluorescent probes from two overlapping microdissection libraries. Probes specific to chromosome regions 1p34–35 and 1p36 were labeled using the ULYSIS Orego

Fluorescence in situ hybridization detected by tyramide signal amplification. Chromosome spreads were prepared from the cultured fibroblast cell line MRC-5 and hybridized with a biotinylated a

Reversible binding by DSB-X biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti–a-tubulin antibody (A11126), detected with either biotin-XX

Subcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells visualized with several fluorescent dyes. Filamentous actin (F-actin) was identified with Alexa Fluor® 633 p

FluoCells® prepared slide #1 (Cat. no. F14780) showing bovine pulmonary artery endothelial cells (BPAEC) that have been labeled with MitoTracker® Red CMXRos (Cat. no. M7512) for mitochondria, and then

Purified bacteriophage T4 replication proteins that have been electrophoresed on a 12% SDS-polyacrylamide gel and then stained with SYPRO® Orange protein gel stain (Cat. no. S6650, S6651). Gel stainin

DiI LDL (Cat. No. L3482) bound to the surface and internalized in the gut of the parasite Schistosoma mansoni. The distribution of LDL in the parasite is used to study a putative mechanism by which th

HeLa cells labeled with anti–golgin-97 antibody and detected using Alexa Fluor® 488 Goat Anti–Mouse IgG antibody. Fixed, permeabilized HeLa cells were labeled with anti–golgin-97 monoclonal antibody (

Capillary endothelial cells and microglia in a rat brain cryosection labeled with the green-fluorescent Alexa Fluor® 488 isolectin IB₄ (Cat. No. I21411). Neurons were identified using the N

Cells were pulsed with BrdU (B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (A21300) and green-fluorescent Alexa Fluo

The subcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells are visualized with several fluorescent dyes. Filamentous actin (F-actin) was identified with Alexa Fluo

A section of mouse intestine was stained with a combination of fluorescent stains. Fibronectin, an extracellular matrix adhesion molecule, was labeled using a chicken primary antibody against fibronec

Actin filaments of fixed bovine pulmonary artery endothelial cells (Cat. no. BPAEC) labeled with Alexa Fluor® 647 phalloidin (Cat. no. A22287). Nuclei were stained with DAPI (Cat. no. D1306, D3571, D2

Peroxisomes were labeled using an antibody directed at peroxisomal membrane protein 70 (PMP-70) and detected with Alexa Fluor® 488 dye–labeled goat anti–mouse IgG secondary antibody. Mitochondria were

Mouse intestine cryosection showing basement membranes labeled with our chicken IgY anti-fibronectin antibody (Cat. No. A21316) and the Alexa Fluor® 488 Goat Anti–Chicken IgG (Cat. No. A11039, green).

Peripheral neurons in mouse intestinal cryosections were labeled with rabbit anti–synapsin I antibody (Cat. No. A6442) and detected using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A1100

Neuronal cells in a 22-hour zebrafish embryo were identified with anti–HuC/HuD mouse monoclonal antibody (Cat. No. A21271) and visualized with red-orange–fluorescent Alexa Fluor® 568 goat anti–mouse I

FluoCells prepared slide #2 (Cat. no. F14781), which shows bovine pulmonary artery endothelial cells (BPAEC) that have been stained with an anti–ß-tubulin mouse monoclonal antibody in conjunction with

FluoCells® prepared slide #3 (Cat. no. F24630) containing a 16 µm cryostat section of mouse kidney stained with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), red-orange–f

The microtubules of fixed HeLa cells labeled with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126) and visualized using Alexa Fluor® 633 goat anti–mouse IgG (Cat. no. A21050), which is pseud

Bull sperm prelabeled with MitoTracker Green FM (M7514) and used for in vitro fertilization of bovine oocytes. After fertilization, eggs with bound or incorporated sperm were fixed in 2% formal

Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation an

Intrinsic fluorescence in a mouse renal artery cross-section. Nuceli were stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490).

Mouse intestine cryosection showing basement membranes labeled with chicken IgY anti-fibronectin antibody and Alexa Fluor® 488 Goat Anti–Chicken IgG antibody (A11039, green). Goblet cells and cryp

Euphyrene spermatozoa from the pupal testis of the gypsy moth (Lymantria dispar) were incubated in a solution of MitoTracker Red CMXRos (Cat. No. M7512). The sample was subsequently fixed and t

DAPI-stained condensed chromatin in PtK2 cells during the later stages of mitosis. DAPI (Cat. no. D1306, D3571, D21490) binds to the minor groove of DNA with significant fluorescence enhancement.

Probes for fluorescence in situ hybridization (FISH) created using the ULYSIS Nucleic Acid Labeling Kits. Centromere probes to chromosome 15, chromosome 17 and chromosome 1 were easily labeled

Chinook salmon embryo (CHSE) cells infected with hematopoietic necrosis virus. Cell nuclei were stained with DAPI (Cat. no. D1306, D3571), and virions were labeled with mouse monoclonal IgG directed a

Microtubules of a fixed bovine pulmonary artery endothelial cell labeled with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126), and detected with Rhodamine Red™-X goat anti–mouse IgG (Cat. n

Fixed, permeabilized NIH 3t3 cells were labeled with an Alexa Fluor® 488 conjugate of lectin HPA from Helix pomatia (edible snail) (Cat. No. L11271) and counterstained with DAPI (Cat. No. D1306

Human metaphase chromosomes stained with DAPI (Cat. no. D1306, D3571, D21490).

lacZ mRNA in transformed mouse fibroblasts (CRE BAG 2 cells) are hybridized with a singly biotinylated, complementary oligonucleotide. Hybrids were then detected by incubation with a streptavid

FluoCells® prepared slide #2 (Cat. no. F14781) showing bovine pulmonary artery endothelial cells probed with anti–bovine a-tubulin mouse monoclonal 236-10501 (Cat. no. A11126) and visualized with BODI

Neural somata from a mouse brain section labeled with NeuroTrace® 500/525 green-fluorescent Nissl stain (Cat. no. N21480). Nuclei are labeled with DAPI (Cat. no. D1306, D3571, D21490). Myelin and othe

A section of mouse intestine stained with a combination of fluorescent stains. Fibronectin, an extracellular matrix adhesion molecule, was labeled using a chicken primary antibody against fibronectin