Tinción de ácidos nucleicos SYTOX™ Green: solución de 5 mM in DMSO.
Tinción de ácidos nucleicos SYTOX™ Green: solución de 5 mM in DMSO.
Citas y referencias (262)
Invitrogen™

Tinción de ácidos nucleicos SYTOX™ Green: solución de 5 mM in DMSO.

El ácido nucleico SYTOX® Green es una excelente contratinción verde fluorescente para cromosomas y núcleo, impermeable para las células vivas,Más información
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Número de catálogoCantidad
S7020250 μL
Número de catálogo S7020
Precio (MXN)
-
Cantidad:
250 μL
El ácido nucleico SYTOX® Green es una excelente contratinción verde fluorescente para cromosomas y núcleo, impermeable para las células vivas, lo que la convierte en un indicador útil de las células muertas en una población.

También está disponible como una solución lista para su uso y a temperatura ambiente estable: Reactivo NucGreen Dead 488 Ready Probes.
Consulte otros accesorios y reactivos de adquisición de imágenes Ready Probes listos para su uso ›

•  Impermeable para células vivas
•  Excitación/emisión: 504/523 nm
•  Uso con láser de iones de argón 488
•  Funciona con células de mamíferos y bacterias grampositivas y gramnegativas

Determinación sencilla de la viabilidad
SYTOX® Green permite una rápida determinación de la viabilidad celular cuando se utilizan citómetros de flujo, microscopios de fluorescencia, fluorímetros y lectores de microplacas de fluorescencia. No atraviesa membranas intactas, pero penetra fácilmente en las membranas comprometidas características de las células muertas. Dado que presenta una fluorescencia > 500 veces superior tras unirse a ácidos nucleicos, una incubación breve de células muertas con SYTOX® Green resultará en una fluorescencia verde brillante con un máximo de emisión de 523 nm cuando se excita con un láser de iones de argón de 488 nm o cualquier otra fuente de 450 a 490 nm. El excepcional brillo de la señal que se produce en las células muertas hace que SYTOX® Green resulte especialmente útil para determinar la viabilidad de células de mamíferos y de bacterias grampositivas y gramnegativas.

Parte de la familia de ensayos y tinciones de viabilidad celular Invitrogen
Las tinciones SYTOX® para células muertas están disponibles en una serie de colores, incluido rojo, azul y naranja. Además, Invitrogen ha incorporado tinciones SYTOX® a una serie de ensayos para apoptosis, viabilidad celular y metabolismo.

Para uso exclusivo en investigación. No diseñado para uso terapéutico o de diagnóstico en animales o humanos.

Enlaces relacionados
•  Obtenga más información sobre todos los productos SYTOX®.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorVerde
Método de detecciónFluorescente
Tipo de colorantePermeabilidad celular
Emisión523 nm
Intervalo de longitud de onda de excitación504 nm
Para utilizar con (equipo)Citómetro de flujo
FormularioSolución
FormatoTubo(s)
Línea de productosSYTOX
Cantidad250 μL
Condiciones de envíoTemperatura ambiente
Volumen (métrico)250 μl
Tipo de etiquetaFluorescente
Tipo de productoTinción de ácidos nucleicos
SubCellular LocalizationNucleicos, ácidos nucleicos, Nucleus
Unit SizeEach
Contenido y almacenamiento
Contiene 1 vial de tinción de ácido nucleico SYTOX™ Green (solución de 5 mM en DMSO).

Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to mount my Qdot secondary-labeled tissue samples in HistoMount mounting medium, but my Qdot 705 conjugate overlaps with your Qnuclear Deep Red nuclear label. What other nuclear label would you recommend, that is compatible with HistoMount mounting medium?

We recommend using SYTOX Green stain, which you can image using a FITC filter and we have shown to be compatible with HistoMount mounting medium. Some have used DAPI, but there have been some issues with slight quenching and spectral shifting of DAPI into green or even red wavelengths.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (262)

Citations & References
Abstract
Use of SYTOX green dye in the flow cytometric analysis of bacterial phagocytosis.
Authors:Gaforio JJ, Serrano MJ, Ortega E, Algarra I, Alvarez de Cienfuegos G
Journal:Cytometry
PubMed ID:12116370
BACKGROUND: Fluorescein isothiocyanate (FITC) is used widely to label the targets used in flow cytometric phagocytosis assays. Unfortunately, the fluorescence intensity of phagocytosed FITC-labeled targets is influenced by changes in intracellular pH level, making quantitative measurements with this fluorophore problematic. We describe the use of SYTOX green nucleic acid stain ... More
Identification and characterization of two subpopulations of Encephalitozoon intestinalis.
Authors:Hoffman RM, Marshall MM, Polchert DM, Jost BH
Journal:Appl Environ Microbiol
PubMed ID:12902292
Microsporidia are obligate intracellular protozoa that have been shown to be pathogenic to most living creatures. The development of in vitro cell culture propagation methods has provided researchers with large numbers of spores and facilitated the study of these organisms. Here, we describe heterogeneity within cell culture-propagated Encephalitozoon intestinalis suspensions. ... More
Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase.
Authors:Horibata Y, Sakaguchi K, Okino N, Iida H, Inagaki M, Fujisawa T, Hama Y, Ito M
Journal:J Biol Chem
PubMed ID:15320336
Endoglycoceramidase (EGCase; EC 3.2.1.123) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. We detected strong EGCase activity in animals belonging to Cnidaria, Mollusca, and Annelida and cloned the enzyme from a hydra, Hydra magnipapillata. The hydra EGCase, consisting of 517 amino acid ... More
Cell death during ischemia: relationship to mitochondrial depolarization and ROS generation.
Authors:Levraut J, Iwase H, Shao ZH, Vanden Hoek TL, Schumacker PT
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:12388276
Ischemia-reperfusion injury induces cell death, but the responsible mechanisms are not understood. This study examined mitochondrial depolarization and cell death during ischemia and reperfusion. Contracting cardiomyocytes were subjected to 60-min ischemia followed by 3-h reperfusion. Mitochondrial membrane potential (DeltaPsi(m)) was assessed with tetramethylrhodamine methyl ester. During ischemia, DeltaPsi(m) decreased to ... More
Up-regulation of cyclooxygenase-2 and apoptosis resistance by p38 MAPK in hypericin-mediated photodynamic therapy of human cancer cells.
Authors:Hendrickx N, Volanti C, Moens U, Seternes OM, de Witte P, Vandenheede JR, Piette J, Agostinis P
Journal:J Biol Chem
PubMed ID:14557269
Photodynamic Therapy (PDT) is an approved anticancer therapy that kills cancer cells by the photochemical generation of reactive oxygen species following absorption of visible light by a photosensitizer, which selectively accumulates in tumors. We report that hypericin-mediated PDT of human cancer cells leads to up-regulation of the inducible cyclooxygenase-2 (COX-2) ... More
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