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The easiest way to image your cellsMolecular Probes ReadyProbes reagents are ready-to-use imaging solutions designed to allow you to stain cells with:
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Reach for the convenient dropper bottle, add 2 drops per milliliter of your cell suspension, and you're ready to image. ReadyProbes reagents address your most common imaging needs, including nuclear and cytoskeletal staining, and analyses for cell viability and apoptosis. In addition, we’ve formulated some of our most popular secondary antibodies for use straight from the dropper bottle. We’ve also developed ReadyProbes imaging accessories to help you get the best results from your staining protocol. Imaging has never been easier.
ReadyProbes Cell Stains
With each ReadyProbes reagent, you can follow the icon-based protocol to obtain quick and easy results for a common imaging application. ReadyProbes cell stains can remain on the bench within arm’s reach of your microscope. Add 2 drops per milliliter of solution, and you're ready for the next step in your experiment.
HeLa cells labeled with NucBlue Live ReadyProbes Reagent and ActinRed 555 ReadyProbes Reagent.
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NucBlue Live ReadyProbes Reagent
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NucRed Live 647 ReadyProbes Reagent
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NucBlue Fixed Cell ReadyProbes Reagent
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|---|---|---|---|
| Readout |
The easiest fluorescent staining method for nucleic acids; no calculations, no dilutions, no pipetting
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| Target |
High-efficiency staining of both RNA and DNA
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| Common filter set |
DAPI
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Cy5
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DAPI
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| Ex/Em (nm) |
358/461
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638/686
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360/460
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| Signal-to-noise ratio |
|
|
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| Photostability |
|
|
|
| Live cells |
Yes
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Yes
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No
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| Fixed cells |
Yes
|
Yes
|
Yes
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| Fixable |
Yes
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Yes
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NA
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| Format |
6 x dropper bottles
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6 x dropper bottles
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6 x dropper bottles
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| Cat. No. |
R37605
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R37106
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R37606
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See more nuclear staining reagents
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ActinGreen 488 ReadyProbes Reagent
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ActinRed 555 ReadyProbes Reagent
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|---|---|---|
| Readout |
The easiest fluorescent staining method for actin; no calculations, no dilutions, no pipetting
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| Target |
High-affinity staining of F-actin
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| Common filter set |
FITC
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TRITC
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| Ex/Em (nm) |
495/518
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555/565
|
| Signal-to-noise ratio |
|
|
| Photostability |
|
|
| Live cells |
No
|
No
|
| Fixed cells |
Yes
|
Yes
|
| Fixable |
NA
|
NA
|
| Platforms |
Imaging
|
Imaging
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| Format |
6 x dropper bottles
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6 x dropper bottles
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| Cat. No. |
R37110
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R37112
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See more actin staining reagents
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NucGreen Dead 488 ReadyProbes Reagent
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NucRed Dead 647 ReadyProbes Reagent
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ReadyProbes Cell Viability Imaging Kit (Blue/Green)
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ReadyProbes Cell Viability Imaging Kit (Blue/Red)
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|
|---|---|---|---|---|
| Readout |
The easiest fluorescent staining method for viability; no calculations, no dilutions, no pipetting
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|||
| Target |
Single-color assays detect dead cells
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Two-color assays detect dead cells and total cells
|
||
|
Dead cells stain green
|
Dead cells stain red
|
Dead cells stain green, live cells appear blue
|
Dead cells stain red, total cells appear blue
|
|
| Fluorescent label |
DNA stain
|
DNA stain
|
DNA stain
|
Propidium iodide
|
|
Hoechst 33342
|
Hoechst 33342
|
|||
| Standard filter set |
FITC
|
Cy®5
|
FITC
|
TRITC/RFP
|
|
DAPI
|
DAPI
|
|||
| Ex/Em (nm) |
504/523
|
642/661
|
504/523
|
535/617
|
|
360/460
|
360/460
|
|||
| Platforms |
Imaging
|
Imaging
|
Imaging
|
Imaging
|
| Format |
6 x dropper bottles
|
6 x dropper bottles
|
6 x dropper bottles
|
6 x dropper bottles
|
| Cat. No. |
R37109
|
R37113
|
R37609
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R37610
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See more cell viability stains
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CellEvent Caspase-3/7 Green ReadyProbes Reagent
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|---|---|
| Readout |
The easiest fluorescent staining method for apoptosis; no calculations, no dilutions, no pipetting
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| Target |
Fluorescence intensity increases with caspase 3/7 activity
|
| Common filter set |
FITC
|
| Ex/Em (nm) |
502/530
|
| Live cells |
Yes
|
| Fixed cells |
No
|
| Fixable |
Yes
|
| Platforms |
Imaging
|
| Format |
1 x dropper bottle
|
| Cat. No. |
R37111
|
See more apoptosis stains
|
NucBlue Live ReadyProbes Reagent
|
NucRed Live 647 ReadyProbes Reagent
|
NucBlue Fixed Cell ReadyProbes Reagent
|
|
|---|---|---|---|
| Readout |
The easiest fluorescent staining method for nucleic acids; no calculations, no dilutions, no pipetting
|
||
| Target |
High-efficiency staining of both RNA and DNA
|
||
| Common filter set |
DAPI
|
Cy5
|
DAPI
|
| Ex/Em (nm) |
358/461
|
638/686
|
360/460
|
| Signal-to-noise ratio |
|
|
|
| Photostability |
|
|
|
| Live cells |
Yes
|
Yes
|
No
|
| Fixed cells |
Yes
|
Yes
|
Yes
|
| Fixable |
Yes
|
Yes
|
NA
|
| Format |
6 x dropper bottles
|
6 x dropper bottles
|
6 x dropper bottles
|
| Cat. No. |
R37605
|
R37106
|
R37606
|
See more nuclear staining reagents
|
ActinGreen 488 ReadyProbes Reagent
|
ActinRed 555 ReadyProbes Reagent
|
|
|---|---|---|
| Readout |
The easiest fluorescent staining method for actin; no calculations, no dilutions, no pipetting
|
|
| Target |
High-affinity staining of F-actin
|
|
| Common filter set |
FITC
|
TRITC
|
| Ex/Em (nm) |
495/518
|
555/565
|
| Signal-to-noise ratio |
|
|
| Photostability |
|
|
| Live cells |
No
|
No
|
| Fixed cells |
Yes
|
Yes
|
| Fixable |
NA
|
NA
|
| Platforms |
Imaging
|
Imaging
|
| Format |
6 x dropper bottles
|
6 x dropper bottles
|
| Cat. No. |
R37110
|
R37112
|
See more actin staining reagents
|
NucGreen Dead 488 ReadyProbes Reagent
|
NucRed Dead 647 ReadyProbes Reagent
|
ReadyProbes Cell Viability Imaging Kit (Blue/Green)
|
ReadyProbes Cell Viability Imaging Kit (Blue/Red)
|
|
|---|---|---|---|---|
| Readout |
The easiest fluorescent staining method for viability; no calculations, no dilutions, no pipetting
|
|||
| Target |
Single-color assays detect dead cells
|
Two-color assays detect dead cells and total cells
|
||
|
Dead cells stain green
|
Dead cells stain red
|
Dead cells stain green, live cells appear blue
|
Dead cells stain red, total cells appear blue
|
|
| Fluorescent label |
DNA stain
|
DNA stain
|
DNA stain
|
Propidium iodide
|
|
Hoechst 33342
|
Hoechst 33342
|
|||
| Standard filter set |
FITC
|
Cy®5
|
FITC
|
TRITC/RFP
|
|
DAPI
|
DAPI
|
|||
| Ex/Em (nm) |
504/523
|
642/661
|
504/523
|
535/617
|
|
360/460
|
360/460
|
|||
| Platforms |
Imaging
|
Imaging
|
Imaging
|
Imaging
|
| Format |
6 x dropper bottles
|
6 x dropper bottles
|
6 x dropper bottles
|
6 x dropper bottles
|
| Cat. No. |
R37109
|
R37113
|
R37609
|
R37610
|
See more cell viability stains
|
CellEvent Caspase-3/7 Green ReadyProbes Reagent
|
|
|---|---|
| Readout |
The easiest fluorescent staining method for apoptosis; no calculations, no dilutions, no pipetting
|
| Target |
Fluorescence intensity increases with caspase 3/7 activity
|
| Common filter set |
FITC
|
| Ex/Em (nm) |
502/530
|
| Live cells |
Yes
|
| Fixed cells |
No
|
| Fixable |
Yes
|
| Platforms |
Imaging
|
| Format |
1 x dropper bottle
|
| Cat. No. |
R37111
|
See more apoptosis stains
ReadyProbes Secondary Antibodies
ReadyProbes secondary antibodies are conjugated with bright and photostable Alexa Fluor™ 488 and Alexa Fluor594 dyes to offer convenient and robust labeling. These Alexa Fluor secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes reagents, these secondary antibodies are provided in a convenient dropper bottle format. No pipetting or dilutions are required. Just add 2 drops per milliliter of buffer to stain your cells, and follow the icon-based protocol.
CAKI cell peroxisomes labeled using a rabbit anti-PMP70 antibody, followed by detection with Alexa Fluor 488 Goat Anti–Rabbit IgG Antibody ReadyProbes Reagent.
See more secondary antibodies
ReadyProbes Imaging Accessories
ReadyProbes imaging accessories are reagents that make sample staining and image analysis much easier. Whether you are new to fluorescence imaging or an experienced user trying to streamline your protocols, ReadyProbes reagents allow you to spend less time preparing solutions and more time analyzing results. These ready-to-use imaging reagents are optimized to fix and permeabilize your cells or reduce nonspecific background signal, so you can move on quickly to the next step.
HeLa cells fixed and permeabilized using the Image-iT Fixation/Permeabilization Kit.
See more live-cell imaging accessories
See more fixed-cell imaging accessories
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For Research Use Only. Not for use in diagnostic procedures.