ReadyProbes Ready-to-Use Imaging Reagents

Reach for the convenient dropper bottle, add 2 drops per milliliter of your cell suspension, and you're ready to image. ReadyProbes reagents address your most common imaging needs, including nuclear and cytoskeletal staining, and analyses for cell viability and apoptosis. In addition, we’ve formulated some of our most popular secondary antibodies for use straight from the dropper bottle. We’ve also developed ReadyProbes imaging accessories to help you get the best results from your staining protocol. Imaging has never been easier.

With each ReadyProbes reagent, you can follow the icon-based protocol to obtain quick and easy results for a common imaging application. ReadyProbes cell stains can remain on the bench within arm’s reach of your microscope. Add 2 drops per milliliter of solution, and you're ready for the next step in your experiment.

HeLa cells labeled with NucBlue Live ReadyProbes Reagent and ActinRed 555 ReadyProbes Reagent.

ReadyProbes secondary antibodies are conjugated with bright and photostable Alexa Fluor™ 488 and Alexa Fluor594 dyes to offer convenient and robust labeling. These Alexa Fluor secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes reagents, these secondary antibodies are provided in a convenient dropper bottle format. No pipetting or dilutions are required. Just add 2 drops per milliliter of buffer to stain your cells, and follow the icon-based protocol.

CAKI cell peroxisomes labeled using a rabbit anti-PMP70 antibody, followed by detection with Alexa Fluor 488 Goat Anti–Rabbit IgG Antibody ReadyProbes Reagent.

ReadyProbes imaging accessories are reagents that make sample staining and image analysis much easier. Whether you are new to fluorescence imaging or an experienced user trying to streamline your protocols, ReadyProbes reagents allow you to spend less time preparing solutions and more time analyzing results. These ready-to-use imaging reagents are optimized to fix and permeabilize your cells or reduce nonspecific background signal, so you can move on quickly to the next step.

HeLa cells fixed and permeabilized using the Image-iT Fixation/Permeabilization Kit.