ReadyProbes Mouse-on-Mouse IgG Blocking Solution blocks endogenous binding when mouse IgG primary antibody is used to label mouse tissue. Most mouse tissues contain mouse IgG antibodies. When an anti-mouse IgG secondary antibody is used on mouse tissue, the antibody cannot distinguish between the endogenous mouse IgG antibodies and the mouse primary antibody used for target protein detection. ReadyProbes Mouse on Mouse IgG Blocking Solution reduces the binding of endogenous antibodies, resulting in increased specific detection in immunohistochemistry (IHC) and immunofluorescence (IF) applications. This reagent is compatible with both fluorescent and chromogenic IHC procedures.

ReadyProbes Mouse-on-Mouse IgG Blocking Solution is provided at a 30X concentration and is diluted to a 1X working concentration (2 drops in 2.5 mL of PBS or TBS). This solution is not used in place of a non-specific blocking solution (e.g., diluted serum), but is used after the tissue has already been blocked with a non-specific solution and before the primary antibody step. Simply add the 1X solution to the tissue and incubate for 30-60 minutes at room temperature.