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FIGURE: 1 / 24
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (R37116) in ICC/IF
CAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (Product # R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (Product # A-21355) for labeling of mitochondria for 30 min... View More
Product Details
R37116
Applications
Tested Dilution
Western Blot (WB)
Two drops per mL
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
Assay-Dependent
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobin
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS with 0.1% BSA
Contains
<0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Cross Adsorption
Against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG
Antibody Form
Whole Antibody
Product Specific Information
No pipetting or dilutions required. Just tip and drip two drops per mL buffer to stain your cells.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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