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Invitrogen™

NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)

Catalog number:  R37606

Related applications:

Cell Analysis

| Cell Analysis

| Cell Analysis

| Cell Analysis

| Cell Analysis

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Description

DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes® Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

Also available: DAPI (4',6-diamidino-2-phenylindole, dihydrochloride) at 12.5X concentration (5 µM) in water.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area• DAPI is excited with UV light and detected through a blue/cyan filter

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
DAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1)..

Suggestions for use
In most cases 2 drops/ml of NucBlue® Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.
• NucBlue® Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue® Fixed Cell Stain is ideal for use with antibody-based applications.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Color: Blue
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Quantity: 6 vial(s)
Cell Permeability: Cell-Impermeant
Detection Method: Fluorescent
Emission: Visible
Form: Liquid
Label or Dye: DAPI
Excitation/Emission: 360⁄460
Excitation: UV
Label Type: Other Label(s) or Dye(s)
Shipping Condition: Room Temperature
Sub Cellular Localization: Nucleus
Product Line: Molecular Probes®

Contents & storage

6 × 2.5 mL dropper bottles

Store at ≤ 25°C.