Get up to 200X more sensitivity in ICC/IHC/FISH for more clarity and definition with new Tyramide SuperBoost™ kits

NucBlue® Fixed Cell ReadyProbes® Reagent

Catalog number: R37606

Molecular Probes™  Related applications: Cell Structure | Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes® Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area• DAPI is excited with UV light and detected through a blue/cyan filter

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
DAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1)..

Suggestions for use
In most cases 2 drops/ml of NucBlue® Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.
• NucBlue® Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue® Fixed Cell Stain is ideal for use with antibody-based applications.
For Research Use Only. Not for use in diagnostic procedures.



BrazilCouponProductLists: Dealers_40
Color: Blue
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Cell Permeability: Cell-Impermeant
Sub-Cellular Localization: Nucleus
Form: Liquid
Quantity: 6 vial(s)
Label or Dye: DAPI
Product Size: 1 kit
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: UV
Excitation⁄Emission (nm): 360⁄460
Shipping Condition: Room Temperature

Contents & storage

6 × 2.5 mL dropper bottles

Store at ≤ 25°C.


Manuals & protocols

Recommended products