PrestoBlue HS and PrestoBlue Cell Viability Reagent

PrestoBlue Cell Viability Reagent is a ready-to-use, non-toxic, resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability.

We have introduced a superior version of this product—the Invitrogen PrestoBlue HS (High Sensitivity) Cell Viability Reagent that retains all the key characteristics that make PrestoBlue a great cell viability, now with improved sensitivity.

See also

Selection guide

  PrestoBlue HS (high sensitivity) Cell Viability Reagent PrestoBlue Cell Viability Reagent
Use
  • Recommended for quick viability determination, only 10 minute incubation
  • Microplate assay
Mechanism of detection
  • Resazurin is converted to fluorescent resorufin
Sensitivity
Reagent purity
  • Highly purified
  • Varying concentrations of resorufin contamination depending on sources of material and manufacturing conditions
Background fluorescence
  • >50% reduction in background fluorescence
  • Increased background fluorescence due to presence of resorufin contamination
Signal-to-background
  • >100% ratio increase
  • Reduced signal-to-background due to presence of resorufin contamination
Assay signal window
  • 2-fold increase in assay signal window compared to the non-HS (high sensitivity) assay
  • Reduced dynamic range due to the presence of resorufin contamination
Cat. No. P50200 A13262

PrestoBlue HS reagent is recommended for quick viability determination (10 minute incubation), whereas alamarBlue HS reagent is recommended in cases of extended viability studies or when using a high cell density.

Learn more about alamarBlue and alamarBlue HS

Dual plot shows decrease in PrestoBlue signal and increase in Cellevent Caspase-3/7 signal with increasing staurosporine

Easy multiplexing of a caspase-3/7 detection reagent and PrestoBlue cell viability reagent using high-throughput screening. CHO-K1 cells were plated in the presence of a dilution series of staurosporine, in phenol red–free complete media. Following incubation, PrestoBlue reagent and CellEvent Caspase-3/7 Green Detection Reagent were added directly to the wells, and the plate was incubated. Fluorescence signals detected were correlated to live and apoptotic cell populations.

Thermo Scientific plate readers

Expertly detect fluorescence with Thermo Scientific plate readers

High-sensitivity fluorescence detection for 96-1,536 samples can be quickly performed on Fluoroskan or Fluoroskan FL Microplate Fluorometer or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.

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