ReadyProbes™ F-Actin Labeling Reagents
Invitrogen™

ReadyProbes™ F-Actin Labeling Reagents

ActinRed™ and ActinGreen™ ReadyProbes™ reagents assist with bright, selective F-actin labeling in fixed cells and tissues. The ready-to-use dropper or dual-use bottle enables dropwise dispensing or direct pipetting for fast, flexible cytoskeleton imaging.
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Catalog NumberDye TypeExcitation Wavelength Range
R37112TRITC (Tetramethylrhodamine Isothiocyanate)552/577
R37110Alexa Fluor™ 488498/520
R37178Alexa Fluor™ 568580/603
R37179Alexa Fluor™ Plus 647658/675
Catalog number R37112
Price (MXN)
8,506.49
Each
Dye Type:
TRITC (Tetramethylrhodamine Isothiocyanate)
Excitation Wavelength Range:
552/577
Price (MXN)
8,506.49
Each

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 ActinGreen (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine F-actin probe (Cat. No. R415) packaged in a dropper bottle.

ActinRed 568 ReadyProbes Reagent is our high-affinity F-actin probe conjugated to our bright, red-fluorescent Alexa Fluor 568 dye. This dye is packaged in an easy to use dropper-optional bottle, allowing end-users to choose either to pipet out of the tube using the classic cap, or utilize the dropper bottle tip for typical ReadyProbes workflows.

ActinRed 647 ReadyProbes Reagent leverages our high-affinity F-actin probe conjugated to our bright Far-Red Alexa Fluor 647 Plus dye, offering enhanced brightness vs. classic Alexa Fluor 647 F-actin probe conjugates, in an easy to use format. ActinRed 647 ReadyProbes Reagent should be stored from 2-8°C for long-term, and also offers the option to leverage pipetting, if desired, via the dropper-optional cap.

These ready-to-use solutions combine superior brightness with exceptional F-actin staining. ReadyProbes actin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityFixed Cells
ColorRed-Orange
Delivery TypeAdd 2 drops (80 μL) per 1 mL of medium
DescriptionActinRed™ 555 ReadyProbes™ Reagent (Rhodamine phalloidin)
Detection MethodFluorescence
Dye TypeTRITC (Tetramethylrhodamine Isothiocyanate)
Emission577 nm
Excitation552 nm
Excitation Wavelength Range552/577
For Use With (Application)Fixed Cell Imaging, High Content Screening, Fluorescence Microscopy
For Use With (Equipment)Confocal Microscope, EVOS™, CellInsight™ Cell Imaging Instruments, Fluorescence Microscope, Flow Cytometer
FormReady-to-use aqueous solution
FormatDropper bottles
No. of Tests75 Tests
Product LineActinRed, ReadyProbes
Quantity2 dropper bottle(s) kit
ReactivityF-actin
TargetF-actin Cytoskeleton
TechniqueCell Paint Assay
Label TypeFluorescent Dye
Product TypeCell Labeling
Sub Cellular LocalizationCytoskeleton
Unit SizeEach
Contents & Storage
•2 x 2.5 mL dropper bottles
•Store at 15°C to 30°C
•Protect from light
•Do not freeze

Frequently asked questions (FAQs)

Do you offer alternative package sizes for phalloidin or ReadyProbes reagents?

Alternative package sizes may be available through a custom request process. Contact Thermo Fisher Scientific customer support or your sales representative to discuss available options for the specific reagent and quantity needed.

Can I use conjugated phalloidin label probes on organoids?

Yes. Conjugated phalloidin labeling probes can be used with organoids when F-actin is accessible after fixation and permeabilization. Because organoids are thicker than monolayer cell cultures, protocol optimization may be needed, including permeabilization conditions, probe incubation time, and probe concentration.

Are conjugated phalloidin reagents antibodies?

No, Phalloidin is not an antibody. It is a bicyclic peptide toxin originally isolated from the Amanita phalloides mushroom and is used to bind and label F-actin in fixed and permeabilized samples.

Are ReadyProbes ActinRed and ActinGreen reagents stable at room temperature?

Yes. ReadyProbes ActinRed and ActinGreen reagents are designed for storage between 15°C and 30°C. Store the reagents according to the product instructions and protect them from light.

Can I use conjugated phalloidin reagents with IHC, FFPE sections, or cryosections?

Yes. Fluorescent phalloidin can be used with appropriately prepared fixed and permeabilized tissue sections, especially cryosections. FFPE processing and deparaffinization can include organic solvents such as xylene or acetone, which may alter F-actin and reduce phalloidin binding. If paraffin sections show weak or no signal, consider cryosections or an anti-actin antibody.

Citations & References (42)

Citations & References
Abstract
Fabrication of high specificity hollow mesoporous silica nanoparticles assisted by Eudragit for targeted drug delivery.
Authors:She X, Chen L, Velleman L, Li C, Zhu H, He C, Wang T, Shigdar S, Duan W, Kong L,
Journal:
PubMed ID:25617610
'Hollow mesoporous silica nanoparticles (HMSNs) are one of the most promising carriers for effective drug delivery due to their large surface area, high volume for drug loading and excellent biocompatibility. However, the non-ionic surfactant templated HMSNs often have a broad size distribution and a defective mesoporous structure because of the ... More
Collaborative Enhancement of Endothelial Targeting of Nanocarriers by Modulating Platelet-Endothelial Cell Adhesion Molecule-1/CD31 Epitope Engagement.
Authors:Chacko AM, Han J, Greineder CF, Zern BJ, Mikitsh JL, Nayak M, Menon D, Johnston IH, Poncz M, Eckmann DM, Davies PF, Muzykantov VR,
Journal:
PubMed ID:26153796
Nanocarriers (NCs) coated with antibodies (Abs) to extracellular epitopes of the transmembrane glycoprotein PECAM (platelet endothelial cell adhesion molecule-1/CD31) enable targeted drug delivery to vascular endothelial cells. Recent studies revealed that paired Abs directed to adjacent, yet distinct epitopes of PECAM stimulate each other's binding to endothelial cells in vitro ... More
Electrochemical behavior of bioactive coatings on cp-Ti surface for dental application.
Authors:Marques ID, Barão VA, da Cruz NC, Yuan JC, Mesquita MF, Ricomini-Filho AP, Sukotjo C, Mathew MT,
Journal:
PubMed ID:26834277
The surface characteristics and electrochemical properties of bioactive coatings produced by plasma electrolytic oxidation (PEO) with calcium, phosphorous, silicon and silver on commercially pure titanium were evaluated. PEO treatment produced a porous oxide layer, which improved the surface topography, and enriched the surface chemistry with bioactive elements, responsible for mimicking ... More
In situ measurement of magnetization relaxation of internalized nanoparticles in live cells.
Authors:Soukup D, Moise S, Céspedes E, Dobson J, Telling ND,
Journal:
PubMed ID:25562356
Magnetization relaxation mechanisms strongly influence how magnetic nanoparticles respond to high-frequency fields in applications such as magnetic hyperthermia. The dominant mechanism depends on the mobility of the particles, which will be affected in turn by their microenvironment. In this study AC susceptometry was used to follow the in situ magnetic ... More
Controlled electromechanical cell stimulation on-a-chip.
Authors:Pavesi A, Adriani G, Rasponi M, Zervantonakis IK, Fiore GB, Kamm RD,
Journal:
PubMed ID:26135970
Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, ... More