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ActinRed™ 555 ReadyProbes™ Reagent

Catalog number:  R37112

Invitrogen™  Related applications: Cell Structure

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ActinRed™ 555 ReadyProbes® Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes® reagents, ActinRed™ 555 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 540/565 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.

See other ReadyProbes® reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, ActinRed™ 555 reagent allows high-contrast discrimination of actin staining.

Suggestions for use
• ActinRed™ 555 reagent may be added directly to fixed cells in full media or buffer solutions
• In most cases 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinRed™ 555 dye is excited with a maximum at 540 nm and has an emission maximum at 565 nm. It is detected through standard TRITC (Rhodamine/DiI/Cy3™/Alexa Fluor® 546) filters.
For Research Use Only. Not for use in diagnostic procedures.



Label or Dye: TRITC (Tetramethylrhodamine Isothiocyanate)
Sub-Cellular Localization: Cytoskeleton
Color: Red-Orange
Detection Method: Fluorescent
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 540⁄565
For Use With (Equipment): Confocal Microscope, EVOS™, Floid® Cell Imaging System, Flow Cytometer, Fluorescence Microscope
Form: Liquid
Product Line: ActinRed™, ReadyProbes®
Product Size: 1 kit

Contents & storage

2 × 2.5 mL dropper bottles

Store at ≤ 25°C.


Manuals & protocols

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