Fabrication of high specificity hollow mesoporous silica nanoparticles assisted by Eudragit for targeted drug delivery.
AuthorsShe X, Chen L, Velleman L, Li C, Zhu H, He C, Wang T, Shigdar S, Duan W, Kong L,
Journal
PubMed ID25617610
'Hollow mesoporous silica nanoparticles (HMSNs) are one of the most promising carriers for effective drug delivery due to their large surface area, high volume for drug loading and excellent biocompatibility. However, the non-ionic surfactant templated HMSNs often have a broad size distribution and a defective mesoporous structure because of the ... More
Collaborative Enhancement of Endothelial Targeting of Nanocarriers by Modulating Platelet-Endothelial Cell Adhesion Molecule-1/CD31 Epitope Engagement.
AuthorsChacko AM, Han J, Greineder CF, Zern BJ, Mikitsh JL, Nayak M, Menon D, Johnston IH, Poncz M, Eckmann DM, Davies PF, Muzykantov VR,
Journal
PubMed ID26153796
Nanocarriers (NCs) coated with antibodies (Abs) to extracellular epitopes of the transmembrane glycoprotein PECAM (platelet endothelial cell adhesion molecule-1/CD31) enable targeted drug delivery to vascular endothelial cells. Recent studies revealed that paired Abs directed to adjacent, yet distinct epitopes of PECAM stimulate each other's binding to endothelial cells in vitro ... More
Electrochemical behavior of bioactive coatings on cp-Ti surface for dental application.
AuthorsMarques ID, Barão VA, da Cruz NC, Yuan JC, Mesquita MF, Ricomini-Filho AP, Sukotjo C, Mathew MT,
Journal
PubMed ID26834277
The surface characteristics and electrochemical properties of bioactive coatings produced by plasma electrolytic oxidation (PEO) with calcium, phosphorous, silicon and silver on commercially pure titanium were evaluated. PEO treatment produced a porous oxide layer, which improved the surface topography, and enriched the surface chemistry with bioactive elements, responsible for mimicking ... More
In situ measurement of magnetization relaxation of internalized nanoparticles in live cells.
AuthorsSoukup D, Moise S, Céspedes E, Dobson J, Telling ND,
Journal
PubMed ID25562356
Magnetization relaxation mechanisms strongly influence how magnetic nanoparticles respond to high-frequency fields in applications such as magnetic hyperthermia. The dominant mechanism depends on the mobility of the particles, which will be affected in turn by their microenvironment. In this study AC susceptometry was used to follow the in situ magnetic ... More
AuthorsPavesi A, Adriani G, Rasponi M, Zervantonakis IK, Fiore GB, Kamm RD,
Journal
PubMed ID26135970
Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, ... More
Distinct Particle Morphologies Revealed through Comparative Parallel Analyses of Retrovirus-Like Particles.
AuthorsMartin JL, Cao S, Maldonado JO, Zhang W, Mansky LM,
JournalJ Virol
PubMed ID27356903
'The Gag protein is the main retroviral structural protein, and its expression alone is usually sufficient for production of virus-like particles (VLPs). In this study, we sought to investigate-in parallel comparative analyses-Gag cellular distribution, VLP size, and basic morphological features using Gag expression constructs (Gag or Gag-YFP, where YFP is ... More
Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single-cell Imaging.
AuthorsLin JR, Fallahi-Sichani M, Chen JY, Sorger PK,
JournalCurr Protoc Chem Biol
PubMed ID27925668
'Cyclic Immunofluorescence (CycIF) is a public-domain method for performing highly multiplexed immunofluorescence imaging using a conventional epifluorescence microscope. It uses simple reagents and existing antibodies to construct images with up to 30 channels by sequential 4- to 6-channel imaging followed by fluorophore inactivation. Three variant methods are described, the most ... More
Combined Structural and Functional Imaging of the Kidney Reveals Major Axial Differences in Proximal Tubule Endocytosis.
Authors
JournalJ Am Soc Nephrol
PubMed ID30301861
A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix.
AuthorsOwen LM, Adhikari AS, Patel M, Grimmer P, Leijnse N, Kim MC, Notbohm J, Franck C, Dunn AR,
JournalMol Biol Cell
PubMed ID28592635
'The ability of cells to impart forces and deformations on their surroundings underlies cell migration and extracellular matrix (ECM) remodeling and is thus an essential aspect of complex, metazoan life. Previous work has resulted in a refined understanding, commonly termed the molecular clutch model, of how cells adhering to flat ... More
Disparate Contributions of Human Retrovirus Capsid Subdomains to Gag-Gag Oligomerization, Virus Morphology, and Particle Biogenesis.
'The capsid domain (CA) of the retroviral Gag protein is a primary determinant of Gag oligomerization, which is a critical step for immature Gag lattice formation and virus particle budding. Although the human immunodeficiency virus type 1 (HIV-1) CA carboxy-terminal domain (CTD) is essential for CA-CA interactions, the CA CTD ... More
A novel multistep method for chondroitin sulphate immobilization and its interaction with fibroblast cells.
AuthorsOzaltin K, Lehocký M, Kuceková Z, Humpolícek P, Sáha P,
JournalMater Sci Eng C Mater Biol Appl
PubMed ID27770972
'Polymeric biomaterials are widely used in medical applications owing to their low cost, processability and sufficient toughness. Surface modification by creating a thin film of bioactive agents is promising technique to enhance cellular interactions, regulate the protein adsorption and/or avoid bacterial infections. Polyethylene is one of the most used polymeric ... More
Enhancement of temozolomide stability by loading in chitosan-carboxylated polylactide-based nanoparticles.
'In the presented work, amphiphilic nanoparticles based on chitosan and carboxy-enriched polylactic acid have been prepared to improve the stability of the pro-drug temozolomide in physiological media by encapsulation. The carrier, with a diameter in the range of 150-180 nm, was able to accommodate up to 800 µg of temozolomide per mg ... More
Dynamical Magnetic Response of Iron Oxide Nanoparticles Inside Live Cells.
'Magnetic nanoparticles exposed to alternating magnetic fields have shown a great potential acting as magnetic hyperthermia mediators for cancer treatment. However, a dramatic and unexplained reduction of the nanoparticle magnetic heating efficiency has been evidenced when nanoparticles are located inside cells or tissues. Recent studies suggest the enhancement of nanoparticle ... More
Perfused 3D angiogenic sprouting in a high-throughput in vitro platform.
'Angiogenic sprouting, the growth of new blood vessels from pre-existing vessels, is orchestrated by cues from within the cellular microenvironment, such as biochemical gradients and perfusion. However, many of these cues are missing in current in vitro models of angiogenic sprouting. We here describe an in vitro platform that integrates ... More
Human amyloid-ß enriched extracts: evaluation of in vitro and in vivo internalization and molecular characterization.
AuthorsPedrero-Prieto CM, Flores-Cuadrado A, Saiz-Sánchez D, Úbeda-Bañón I, Frontiñán-Rubio J, Alcaín FJ, Mateos-Hernández L, de la Fuente J, Durán-Prado M, Villar M, Martínez-Marcos A, Peinado JR
JournalAlzheimers Res Ther
PubMed ID31253170
'Intracerebral inoculation of extracts from post-mortem human Alzheimer''s disease brains into mice produces a prion-like spreading effect of amyloid-ß. The differences observed between these extracts and the synthetic peptide, in terms of amyloid-ß internalization and seed and cell-to-cell transmission of cytosolic protein aggregates, suggest that brain extracts contain key contributors ... More
The miRNA-21-5p Payload in Exosomes from M2 Macrophages Drives Tumor Cell Aggression via PTEN/Akt Signaling in Renal Cell Carcinoma.
Authors
JournalInt J Mol Sci
PubMed ID35328425
Targeting an anchored phosphatase-deacetylase unit restores renal ciliary homeostasis.
Authors
JournalElife
PubMed ID34250905
Quantitative proteomics reveals key roles for post-transcriptional gene regulation in the molecular pathology of facioscapulohumeral muscular dystrophy.
Authors
JournalElife
PubMed ID30644821
Nature vs. Nurture: Defining the Effects of Mesenchymal Stromal Cell Isolation and Culture Conditions on Resiliency to Palmitate Challenge.
Authors
JournalFront Immunol
PubMed ID31134100
Hedgehog Signaling Regulates Epithelial Morphogenesis to Position the Ventral Embryonic Midline.
Authors
JournalDev Cell
PubMed ID32437643
Deacetylation of Miro1 by HDAC6 blocks mitochondrial transport and mediates axon growth inhibition.
Authors
JournalJ Cell Biol
PubMed ID31068376
Cytokinetic bridge triggers de novo lumen formation in vivo.
Authors
JournalNat Commun
PubMed ID32152267
Activation of Piezo1 sensitizes cells to TRAIL-mediated apoptosis through mitochondrial outer membrane permeability.
Authors
JournalCell Death Dis
PubMed ID31685811
Farnesoid X Receptor Protects against Kidney Injury in Uninephrectomized Obese Mice.
AuthorsGai Z, Gui T, Hiller C, Kullak-Ublick GA,
JournalJ Biol Chem
PubMed ID26655953
Activation of the farnesoid X receptor (FXR) has indicated a therapeutic potential for this nuclear bile acid receptor in the prevention of diabetic nephropathy and obesity-induced renal damage. Here, we investigated the protective role of FXR against kidney damage induced by obesity in mice that had undergone uninephrectomy, a model ... More
Rhinosporidium seeberi Nuclear Cycle Activities Using Confocal Microscopy.
AuthorsDelfino D, Mendoza L, Vilela R
JournalJ Parasitol
PubMed ID26461427
Rhinosporidium seeberi is an uncultivated Ichthyosporean infecting animals, including humans. Recent studies suggested R. seeberi undergoes synchronized nuclear division without cytokinesis. We used confocal microscopy to investigate R. seeberi nuclear division cycles in formalin-fixed tissues stained with DAPI and phalloidin. We report that R. seeberi nuclei in juvenile and intermediary ... More
Biomimetic coatings enhance tribocorrosion behavior and cell responses of commercially pure titanium surfaces.
AuthorsMarques Ida S, Alfaro MF, Saito MT, da Cruz NC, Takoudis C, Landers R, Mesquita MF, Nociti Junior FH, Mathew MT, Sukotjo C, Barão VA,
JournalBiointerphases
PubMed ID27514370
Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stability under ... More
MALT1 Protease Activation Triggers Acute Disruption of Endothelial Barrier Integrity via CYLD Cleavage.
AuthorsKlei LR, Hu D, Panek R, Alfano DN, Bridwell RE, Bailey KM, Oravecz-Wilson KI, Concel VJ, Hess EM, Van Beek M, Delekta PC, Gu S, Watkins SC, Ting AT, Gough PJ, Foley KP, Bertin J, McAllister-Lucas LM, Lucas PC,
JournalCell Rep
PubMed ID27681433
Microvascular endothelial cells maintain a tight barrier to prevent passage of plasma and circulating immune cells into the extravascular tissue compartment, yet endothelial cells respond rapidly to vasoactive substances, including thrombin, allowing transient paracellular permeability. This response is a cornerstone of acute inflammation, but the mechanisms responsible are still incompletely ... More
Endothelial cells release ATP in response to fluid shear stress, which activates purinergic (P2) receptor-mediated signaling molecules including endothelial nitric oxide (eNOS), a regulator of vascular tone. While P2 receptor-mediated signaling in the vasculature is well studied, the role of P2Y ... More
Fast axial scanning for 2-photon microscopy using liquid lens technology.
AuthorsTehrani KF, Sun MK, Karumbaiah L, Mortensen LJ,
JournalProc SPIE Int Soc Opt Eng
PubMed ID29706682
Scanning microscopy methods require movement of the focus in Z coordinates to produce an image of a 3-dimensional volume. In a typical imaging system, the optical setup is kept fixed and either the sample or the objective is translated with a mechanical stage driven by a stepper motor or a ... More
96 perfusable blood vessels to study vascular permeability in vitro.
Authorsvan Duinen V, van den Heuvel A, Trietsch SJ, Lanz HL, van Gils JM, van Zonneveld AJ, Vulto P, Hankemeier T,
JournalSci Rep
PubMed ID29273771
Current in vitro models to test the barrier function of vasculature are based on flat, two-dimensional monolayers. These monolayers do not have the tubular morphology of vasculature found in vivo and lack important environmental cues from the cellular microenvironment, such as interaction with an extracellular matrix (ECM) and exposure to ... More
Differentiating Staphylococcus aureus from Escherichia coli mastitis: S. aureus triggers unbalanced immune-dampening and host cell invasion immediately after udder infection.
The etiology determines quality and extent of the immune response after udder infection (mastitis). Infections with Gram negative bacteria (e.g. Escherichia coli) will quickly elicit strong inflammation of the udder, fully activate its immune defence via pathogen receptor driven activation of I?B/NF-?B signaling. This often eradicates the pathogen. In contrast, ... More
Anti-invasive effects of CXCR4 and FAK inhibitors in non-small cell lung carcinomas with mutually inactivated p53 and PTEN tumor suppressors.
AuthorsDragoj M, Bankovic J, Sereti E, Stojanov SJ, Dimas K, Pesic M, Stankovic T,
JournalInvest New Drugs
PubMed ID28733702
Non-small cell lung carcinoma (NSCLC) is the most common type of lung cancer. At the time of diagnosis, a large percentage of NSCLC patients have already developed metastasis, responsible for extremely high mortality rates. CXCR4 receptor and focal adhesion kinase (FAK) are known to regulate such invasive cancer behavior. Their ... More
Tuning the interactions between chiral plasmonic films and living cells.
AuthorsZhao X, Xu L, Sun M, Ma W, Wu X, Xu C, Kuang H,
JournalNat Commun
PubMed ID29222410
Designing chiral materials to manipulate the biological activities of cells has been an important area not only in chemistry and material science, but also in cell biology and biomedicine. Here, we introduce monolayer plasmonic chiral Au nanoparticle (NP) films modified with L- or D-penicillamine (Pen) to be developed for cell ... More
Giardiasis is a common diarrheal disease caused by the protozoan parasite Giardia intestinalis. Cysteine proteases (CPs) are acknowledged as virulence factors in Giardia but their specific role in the molecular pathogenesis of disease is not known. Herein, we aimed to characterize the three main secreted CPs (CP14019, CP16160 and CP16779), ... More
Single Cell Analysis Identifies the miRNA Expression Profile of a Subpopulation of Muscle Precursor Cells Unique to Humans With Type 2 Diabetes.
MicroRNAs (miRNAs) take part in regulating central cellular processes such as differentiation and metabolism. We have previously shown that muscle progenitor cells derived from individuals with type 2 diabetes (T2DM) have a dysregulated miRNA profile. We hypothesized that the T2DM muscle progenitor population is heterogeneous in its miRNA expression and ... More
Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy.
AuthorsZhou Y, Saito M, Miyamoto T, Novak P, Shevchuk AI, Korchev YE, Fukuma T, Takahashi Y,
JournalAnal Chem
PubMed ID29345134
Primary cilia are hair-like sensory organelles whose dimensions and location vary with cell type and culture condition. Herein, we employed scanning ion conductance microscopy (SICM) to visualize the topography of primary cilia from different cell types. By combining SICM with fluorescence imaging, we successfully distinguished between surface cilia that project ... More
Gravin regulates centrosome function through PLK1.
AuthorsColicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula L, Hehnly H,
JournalMol Biol Cell
PubMed ID29282278
We propose to understand how the mitotic kinase PLK1 drives chromosome segregation errors, with a specific focus on Gravin, a PLK1 scaffold. In both three-dimensional primary prostate cancer cell cultures that are prone to Gravin depletion and Gravin short hairpin RNA (shRNA)-treated cells, an increase in cells containing micronuclei was ... More
Chlorination and oxidation of human plasma fibronectin by myeloperoxidase-derived oxidants, and its consequences for smooth muscle cell function.
AuthorsNybo T, Cai H, Chuang CY, Gamon LF, Rogowska-Wrzesinska A, Davies MJ,
JournalRedox Biol
PubMed ID30237127
Fibronectin (FN) occurs as both a soluble form, in plasma and at sites of tissue injury, and a cellular form in tissue extracellular matrices (ECM). FN is critical to wound repair, ECM structure and assembly, cell adhesion and proliferation. FN is reported to play a critical role in the development, ... More
Salinomycin-loaded Nanofibers for Glioblastoma Therapy.
AuthorsNorouzi M, Abdali Z, Liu S, Miller DW,
JournalSci Rep
PubMed ID29925966
Salinomycin is an antibiotic that has recently been introduced as a novel and effective anti-cancer drug. In this study, PLGA nanofibers (NFs) containing salinomycin (Sali) were fabricated by electrospinning for the first time. The biodegradable PLGA NFs had stability for approximately 30 days and exhibited a sustained release of the drug ... More
Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages.
AuthorsPopena I, Abols A, Saulite L, Pleiko K, Zandberga E, Jekabsons K, Endzelinš E, Llorente A, Line A, Riekstina U,
JournalCell Commun Signal
PubMed ID29690889
Macrophages are one of the most important players in the tumor microenvironment. The polarization status of tumor associated macrophages into a pro-inflammatory type M1 or anti-inflammatory type M2 may influence cancer progression and patient survival. Extracellular vesicles (EVs) are membrane-bound vesicles containing different biomolecules that are involved in cell to ... More
Exosomes from human-bone-marrow-derived mesenchymal stem cells protect against renal ischemia/reperfusion injury via transferring miR-199a-3p.
AuthorsZhu G, Pei L, Lin F, Yin H, Li X, He W, Liu N, Gou X
JournalJ Cell Physiol
PubMed ID31180587
Renal ischemia/reperfusion (I/R) injury is the main reason for acute kidney injury (AKI) and is closely related to high morbidity and mortality. In this study, we found that exosomes from human-bone-marrow-derived mesenchymal stem cells (hBMSC-Exos) play a protective role in hypoxia/reoxygenation (H/R) injury. hBMSC-Exos were enriched in miR-199a-3p, and hBMSC-Exo ... More
The Absence of Tryptase Mcpt6 Causes Elevated Cellular Stress in Response to Modulation of the Histone Acetylation Status in Mast Cells.
AuthorsSantosh Martin S, Rabelo Melo F, Pejler G
JournalCells
PubMed ID31581668
Mast cells contain large amounts of proteases stored within their secretory granules. Previously we showed that one of these proteases, tryptase, in addition to its location within granules, can also be found within the mast cell nucleus, where it has the capacity to affect the acetylation profile of nucleosomal core ... More