Image-iT™ FX Signal Enhancer ReadyProbes™ Reagenz

Zitierungen und Referenzen (10)

Invitrogen™

Image-iT™ FX Signal Enhancer ReadyProbes™ Reagenz

Katalognummer	Menge
R37107	6 x 2.5 mL kit

Katalognummer R37107

Preis (EUR)

318,00

Menge:

6 x 2.5 mL kit

Preis (EUR)

318,00

Image-iT™ FX Signal Enhancer ReadyProbes™ Reagenz reduziert erheblich die Hintergrundfluoreszenz, die durch unspezifische Färbung während des Sekundärnachweises, mithilfe von Konjugaten von Streptavidin oder sekundären Antikörpern, entsteht. Das Reagenz wird direkt auf Objektträger oder Deckgläser mit fixierten und permeabilisierten Zell- oder Gewebeproben vor dem Anfärben mit fluoreszierenden Sonden aufgetragen.

• Einfaches Protokoll —gebrauchsfertige Lösung mit einer Tropfflasche anwenden
• Kompatibel mit den anderen Schritten in Ihrem Immunfärbeprotokoll
• Effektiv mit den meisten fluoreszierenden Markern
• Stabilität bei Raumtemperatur — griffbereite Aufbewahrung an Ihrem Arbeitsplatz oder Zellkulturbereich

Weitere Reagenzien von ReadyProbes™ für die Zellfärbung
Finden Sie weitere Werkzeuge für die Bildgebung mit fixierten Zellen

Anwendungen zur zellulären Bildgebung
Image-iT™ FX Signal Enhancer Reagenz ist ein hochwirksames Produkt, das die Hintergrundfärbung blockiert, die durch die unspezifischen Wechselwirkungen einer Vielzahl von Fluoreszenzfarbstoffen mit Zell- und Gewebebestandteilen entsteht. Die Hintergrundfärbung, die bei fluoreszierenden Konjugaten von Streptavidin und Antikörperkonjugaten beobachtet wird, wird weitgehend eliminiert, wenn das Image-iT™ FX Signal Enhancer Reagenz vor der Färbung auf fixierte und permeabilisierte Zellen aufgetragen wird.

Anwendungsvorschläge
•: Tragen Sie Image-iT™ FX Signal Enhancer Reagenz vor der Immunfärbung direkt auf Objektträger oder Deckgläser mit Zell- oder Gewebeproben auf.
• Im Anschluss an das Waschen nach den Fixierungs-/Permeabilisierungsschritten tropfen Sie einfach ausreichend Reagenz auf die Probe, um sie zu bedecken, und inkubieren Sie sie für 30 Minuten. Fahren Sie dann mit Ihrem üblichen Färbeprotokoll fort.
• Bei Bedarf können nach dem Blockieren mit dem Image-iT™ Signal Enhancer ReadyProbes™ Reagenz weitere Blockierungsschritte durchgeführt werden

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

FormatBottle(s)

ProduktlinieImage-iT, ReadyProbes

Menge6 x 2.5 mL kit

ProdukttypFX-Signalverstärkerreagenz

LösungstypSignalverstärker

Unit SizeEach

Inhalt und Lagerung

6 × 2,5 ml-Tropfflaschen

Bei ≤25 °C lagern.

Häufig gestellte Fragen (FAQ)

I used Image-iT FX Signal Enhancer solution to get rid of nonspecific nuclear labeling with Alexa Fluor 568 secondary antibody, but I also saw a significant reduction in my specific mitochondrial antibody labeling. Why is this and what can I do?

The Image-iT FX Signal Enhancer reduces non-specific binding of dye conjugates by blocking positively-charged areas of cells or tissues that attract negatively-charged dyes. In cells after fixation, some positively-charged structures are the nuclei and mitochondria. Thus, you would expect to see a reduction in both mitochondrial and nuclear signal. The lower signal you see afterward is the specific mitochondrial signal; the fluorescence that was lost was the non-specific labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Image-iT FX Signal Enhancer instead of my normal blocking solution (BSA or serum)?

No. Image-iT FX Signal Enhancer is not a protein blocker, like BSA, normal serum, or other commercial antibody blockers. Use it as a separate step to block non-specific charge-based binding of dyes to cellular components.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am labeling fixed and permeabilized cultured cells with an Alexa Fluor secondary antibody. My secondary-only control is showing nuclear and mitochondrial labeling, even though I did a thorough protein blocking and tried a concentration range. What can be done to minimize this non-specific binding?

This is most likely charge-based binding due to interactions of the charge on the dyes with cellular components of opposite charge. This can be blocked by using the Image-iT FX Signal Enhancer Solution which eliminates non-specific binding due to charge. The Signal Enhancer is applied as a pre-blocking step and your regular blocking regimen should be used to limit non-specific binding due to protein-protein interactions. Signal Enhancer cannot be used on live cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am observing high background when I analyze my click-labeled samples. What is causing this and what can I do to reduce the background?

The click reaction is very selective between an azide and alkyne. No other side reactions are possible in a biological system. Any non-specific background is due to non-covalent binding of the dye to various cellular components. The Select FX Signal Enhancer is not effective at reducing non-specific charge-based binding of dyes following the click reaction; we do not recommend its use with the Click-iT detection reagents. The best method to reduce background is to increase the number of BSA washes. You should always do a no-dye or no-click reaction control under the same processing and detection conditions to verify that the background is actually due to the dye and not autofluorescence. You can also perform the complete click reaction on a carrier solvent-only, no EdU or no-EU control to verify the specificity of the click reaction signal.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

View all Image-iT™ FX Signal Enhancer ReadyProbes™ Reagenz FAQs

Zitierungen und Referenzen (10)

Zitierungen und Referenzen

Abstract

Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells.

Authors:Desroches BR, Zhang P, Choi BR, King ME, Maldonado AE, Li W, Rago A, Liu G, Nath N, Hartmann KM, Yang B, Koren G, Morgan JR, Mende U,

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:22427522

To bridge the gap between two-dimensional cell culture and tissue, various three-dimensional (3-D) cell culture approaches have been developed for the investigation of cardiac myocytes (CMs) and cardiac fibroblasts (CFs). However, several limitations still exist. This study was designed to develop a cardiac 3-D culture model with a scaffold-free technology ... More

Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.

Authors:Hong J, Xu M, Li R, Cheng YS, Kouznetsova J, Beers J, Liu C, Zou J, Zheng W

Journal:Stem Cell Res

PubMed ID:31071499

'Mucopolysaccharidosis Type II (MPS II), also known as Hunter syndrome, is a rare X-linked genetic disease caused by mutations in the IDS gene encoding iduronate 2-sulfatase (I2S). This is a multisystem disorder with significant variation in symptoms. Here, we document a human induced pluripotent stem cell (iPSC) line generated from ... More

Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes.

Authors:

Journal:Nat Methods

PubMed ID:34059829

The aberrant upregulation of exon 10-inclusive SREK1 through SRSF10 acts as an oncogenic driver in human hepatocellular carcinoma.

Authors:

Journal:Nat Commun

PubMed ID:35296659

HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses.

Authors:

Journal:Cell Host Microbe

PubMed ID:33571449

View all Image-iT™ FX Signal Enhancer ReadyProbes™ Reagenz citations