Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells.
AuthorsDesroches BR, Zhang P, Choi BR, King ME, Maldonado AE, Li W, Rago A, Liu G, Nath N, Hartmann KM, Yang B, Koren G, Morgan JR, Mende U,
JournalAm J Physiol Heart Circ Physiol
PubMed ID22427522
To bridge the gap between two-dimensional cell culture and tissue, various three-dimensional (3-D) cell culture approaches have been developed for the investigation of cardiac myocytes (CMs) and cardiac fibroblasts (CFs). However, several limitations still exist. This study was designed to develop a cardiac 3-D culture model with a scaffold-free technology ... More
Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.
AuthorsHong J, Xu M, Li R, Cheng YS, Kouznetsova J, Beers J, Liu C, Zou J, Zheng W
JournalStem Cell Res
PubMed ID31071499
'Mucopolysaccharidosis Type II (MPS II), also known as Hunter syndrome, is a rare X-linked genetic disease caused by mutations in the IDS gene encoding iduronate 2-sulfatase (I2S). This is a multisystem disorder with significant variation in symptoms. Here, we document a human induced pluripotent stem cell (iPSC) line generated from ... More
The aberrant upregulation of exon 10-inclusive SREK1 through SRSF10 acts as an oncogenic driver in human hepatocellular carcinoma.
Authors
JournalNat Commun
PubMed ID35296659
HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses.
Authors
JournalCell Host Microbe
PubMed ID33571449
Extracellular HSP110 skews macrophage polarization in colorectal cancer.
Authors
JournalOncoimmunology
PubMed ID27622020
Spontaneous Hair Cell Regeneration Is Prevented by Increased Notch Signaling in Supporting Cells.
AuthorsMcGovern MM, Zhou L, Randle MR, Cox BC
JournalFront Cell Neurosci
PubMed ID29780306
During embryonic development, differentiation of cochlear progenitor cells into hair cells (HCs) or supporting cells (SCs) is partially controlled through Notch signaling. Many studies have shown that inhibition of Notch signaling allows SCs to convert into HCs in both normal and drug damaged neonatal mouse cochleae. This mechanism is also ... More
Optimization of prostate cancer cell detection using multiplex tyramide signal amplification.
Approximately 29?000 men die of prostate cancer (PCa) each year in the United States, and 90% to 100% of them are due to incurable bone metastasis. It is difficult to determine (1) when PCa disseminates in the natural history of the disease; (2) where cancer cell disseminates before becoming overt ... More
Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids.
AuthorsAsaoka Y, Morita H, Furumoto H, Heisenberg CP, Furutani-Seiki M
JournalMethods Mol Biol
PubMed ID30565134
The transcription coactivator, Yes-associated protein (YAP), which is a nuclear effector of the Hippo signaling pathway, has been shown to be a mechano-transducer. By using mutant fish and human 3D spheroids, we have recently demonstrated that YAP is also a mechano-effector. YAP functions in three-dimensional (3D) morphogenesis of organ and ... More
An induced pluripotent stem cell line (TRNDi001-D) from a Niemann-Pick disease type C1 (NPC1) patient carrying a homozygous p. I1061T (c. 3182T>C) mutation in the NPC1 gene.
AuthorsLi R, Pradhan M, Xu M, Roeder A, Beers J, Zou J, Liu C, Porter FD, Zheng W
JournalStem Cell Res
PubMed ID32114296
Niemann-Pick disease, type C (NPC) is a rare autosomal recessive genetic disease caused by mutations in either NPC1 or NPC2, which encodes an intracellular cholesterol-binding protein in lysosome. Deficiency of either NPC1 or NPC2 protein results in malfunction of intracellular cholesterol trafficking and lysosomal accumulation of unesterified cholesterols. A human ... More