EVOS Light Cube Selection Guide for EVOS and Countess II FL Instruments
Light Cubes |
Specialty Light Cubes
|
||||||
---|---|---|---|---|---|---|---|
| DAPI |
| RFP |
| CFP-YFP |
| Qdot 605 |
| TagBFP |
| Texas Red |
| AO |
| Qdot 625 |
| CFP |
| Cy5 |
| AOred |
| Qdot 655 |
| GFP |
| Cy7 |
| Qdot 525 |
| Qdot 705 |
| YFP |
| Qdot 545 |
| Qdot 800 | ||
| Qdot 565 |
| Qdot 525-800 | ||||
| Qdot 585 |
DAPI (357/44 nm Excitation; 447/60 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
1,8-ANS | 372 | 480 | ![]() |
|
4-methylumbelliferone | 360 | 440 | ![]() |
|
7-amino-4-methylcoumarin (AMC) | 351 | 430 | ![]() |
|
7-hydroxy-4-methylcoumarin | 360 | 449 | ![]() |
|
ABQ | 344 | 445 | ![]() |
|
Acridine | 362 | 462 | ![]() |
|
Alexa Fluor 350 | 346 | 442 | ![]() |
|
Alexa Fluor 405 | 401 | 421 | ![]() |
|
AMCA | 345 | 425 | ![]() |
|
AMCA-X | 353 | 442 | ![]() |
|
BFP | 382 | 448 | ![]() |
|
CellTrace Calcein Blue | 373 | 440 | ![]() |
|
CellTrace Calcein Violet | 401 | 451 | ![]() |
|
Calcofluor White | 350 | 440 | ![]() |
|
Cascade Blue | 377 | 420 | ![]() |
|
Dansyl | 335 | 525 | ![]() |
|
DAPI | 345 | 455 | ![]() |
|
DiFMU (pH 9.0) | 357 | 450 | ![]() |
|
DyLight 350 | 353 | 432 | ![]() |
|
ER-Tracker Blue-White DPX | 371 | 554 | ![]() |
|
Fast Blue | 360 | 440 | ![]() |
|
FluoSpheres Blue | 356 | 412 | ![]() |
|
Hoechst 33258 | 345 | 487 | ![]() |
|
Hoechst 33342 | 347 | 483 | ![]() |
|
LIVE/DEAD Fixable Blue Dead Cell Stain | 344 | 442 | ![]() |
|
LysoSensor Blue | 374 | 424 | ![]() |
|
LysoTracker Blue | 373 | 422 | ![]() |
|
Marina Blue | 365 | 460 | ![]() |
|
Methoxycoumarin | 360 | 410 | ![]() |
|
Monochlorobimane(mBCI) | 380 | 461 | ![]() |
|
NucBlue Fixed Cell Stain | 345 | 455 | ![]() |
|
NucBlue Live Cell Stain | 347 | 483 | ![]() |
|
Pacific Blue | 405 | 455 | ![]() |
|
PBFI | 360 | 420 | ![]() |
|
Phorwite AR | 360 | 430 | ![]() |
|
Quinine Sulfate | 349 | 461 | ![]() |
|
sgBFP (Super Glow BFP) | 387 | 450 | ![]() |
|
SITS | 365 | 460 | ![]() |
|
SpectrumBlue | 405 | 449 | ![]() |
|
Thioflavin TCN | 350 | 460 | ![]() |
|
Thiolyte | 378 | 483 | ![]() |
|
True Blue | 365 | 425 | ![]() |
|
Uvitex SFC | 365 | 435 | ![]() |
|
Y66H | 360 | 442 | ![]() |
TagBFP (390/18 nm Excitation; 447/60 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 405 | 401 | 421 | ![]() |
|
DyLight 405 | 400 | 420 | ![]() |
|
BFP | 382 | 448 | ![]() |
|
Cascade Blue | 400 | 420 | ![]() |
|
Pacific Blue | 410 | 455 | ![]() |
|
SpectrumBlue | 400 | 450 | ![]() |
|
TagBFP | 402 | 457 | ![]() |
CFP (445/45 nm Excitation; 510/42 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Auramine O | 445 | 500 | ![]() |
|
CellLight CFP | 434 | 477 | ![]() |
|
ECFP | 434 | 477 | ![]() |
|
Evans Blue | 460 | 515 | ![]() |
|
Lucifer yellow | 428 | 544 | ![]() |
GFP (470/22 nm Excitation; 510/42 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
5-carboxyfluorescein(5-FAM) | 492 | 518 | ![]() |
|
Acridine Orange + DNA | 500 | 526 | ![]() |
|
ActinGreen (Alexa Fluor 488 phalloidin) | 496 | 518 | ![]() |
|
Alexa Fluor 430 | 432 | 540 | ![]() |
|
Alexa Fluor 488 | 496 | 518 | ![]() |
|
ATTO 465 | 453 | 507 | ![]() |
|
Auramine | 460 | 540 | ![]() |
|
Aurophosphine | 470 | 540 | ![]() |
|
BCECF | 499 | 540 | ![]() |
|
BODIPY 492/515 | 490 | 540 | ![]() |
|
BODIPY 505/515 | 502 | 540 | ![]() |
|
BODIPY FL | 502 | 540 | ![]() |
|
BTC | 464 | 540 | ![]() |
|
Calcein | 494 | 540 | ![]() |
|
Calcium Green-1 | 506 | 540 | ![]() |
|
Catskill Green 540 | 482 | 540 | ![]() |
|
CellLight GFP | 488 | 507 | ![]() |
|
CellEvent Caspase-3/7 Green | 488 | 507 | ![]() |
|
CellTracker Green | 490 | 540 | ![]() |
|
CellRox Green | 485 | 520 | ![]() |
|
CFDA | 494 | 540 | ![]() |
|
CFP | 434 | 540 | ![]() |
|
Click-iT EdU Alexa Fluor 488 | 496 | 518 | ![]() |
|
Cy2 | 492 | 540 | ![]() |
|
CyQUANT Direct (CyQUANT GR) | 500 | 540 | ![]() |
|
DAF-FM | 493 | 540 | ![]() |
|
DiO (Vybrant DiO) | 489 | 506 | ![]() |
|
Diversa Green-FP | 484 | 507 | ![]() |
|
DyLight+C110 488 | 493 | 518 | ![]() |
|
Emerald Green | 490 | 540 | ![]() |
|
Evans Blue | 460 | 515 | ![]() |
|
FITC (Fluorescein) | 492 | 520 | ![]() |
|
Fluo-3 | 506 | 540 | ![]() |
|
Fluo-4 | 494 | 540 | ![]() |
|
Fluoro-Emerald | 494 | 518 | ![]() |
|
Fluoro-Jade B | 480 | 525 | ![]() |
|
Fluor-X | 494 | 520 | ![]() |
|
FluoSpheres Yellow-Green | 501 | 513 | ![]() |
|
GFP (EGFP) | 488 | 507 | ![]() |
|
H2DCFDA (H2-DCF,DCFR) | 504 | 540 | ![]() |
|
HCS LipidTox Green | 498 | 506 | ![]() |
|
HPTS (Solvent Green 7) | 455 | 510 | ![]() |
|
LIVE/DEAD Fixable Green | 498 | 526 | ![]() |
|
Lucifer Yellow | 423 | 543 | ![]() |
|
LysoSensor Green | 448 | 503 | ![]() |
|
LysoTracker Green | 504 | 511 | ![]() |
|
Magnesium Green | 506 | 530 | ![]() |
|
mHoneydew | 478 | 561 | ![]() |
|
MitoTracker Green FM | 490 | 512 | ![]() |
|
NBD Amine | 450 | 530 | ![]() |
|
NBD-X | 467 | 538 | ![]() |
|
NeuroTrace 500/525 Green | 497 | 524 | ![]() |
|
Nitrobenzoxadiazole | 465 | 510 | ![]() |
|
NucGreen Dead Cell Stain | 504 | 523 | ![]() |
|
Oregon Green 488 | 498 | 524 | ![]() |
|
Oregon Green 514 | 512 | 532 | ![]() |
|
pHrodo Green | 509 | 533 | ![]() |
|
Premo FUCCI Cell Cycle Sensor (S/G2/M phases) | 474 | 509 | ![]() |
|
Qdot 525 | UV | 525 | ![]() |
|
Qdot 545 | UV | 543 | ![]() |
|
Quant-iT PicoGreen | 502 | 522 | ![]() |
|
Rhodamine 110 | 496 | 520 | ![]() |
|
Rhodamine 123 | 507 | 529 | ![]() |
|
Rhodamine Green | 497 | 523 | ![]() |
|
Rhodol Green | 496 | 523 | ![]() |
|
rsGFP (red shifted GFP, S65T) | 498 | 516 | ![]() |
|
S65C | 479 | 507 | ![]() |
|
S65L | 484 | 510 | ![]() |
|
S65T | 488 | 511 | ![]() |
|
sgGFP (Super Glow GFP) | 474 | 509 | ![]() |
|
Sodium Green | 506 | 532 | ![]() |
|
SpectrumGreen | 498 | 522 | ![]() |
|
Surf Green EX | 469 | 526 | ![]() |
|
SYBR Gold | 495 | 539 | ![]() |
|
SYBR Green I | 497 | 521 | ![]() |
|
SYBR Safe | 507 | 524 | ![]() |
|
SYTO 9 | 482 | 500 | ![]() |
|
SYTO 11 | 506 | 525 | ![]() |
|
SYTO 13 | 487 | 505 | ![]() |
|
SYTO 16 | 490 | 520 | ![]() |
|
SYTO 45 | 452 | 484 | ![]() |
|
SYTO RNASelect | 503 | 527 | ![]() |
|
SYTOX Green | 504 | 523 | ![]() |
|
TET | 520 | 541 | ![]() |
|
TO-PRO-1 | 515 | 530 | ![]() |
|
TOTO-1 | 513 | 531 | ![]() |
|
Vybrant DyeCycle Green | 505 | 532 | ![]() |
|
wtGFP (wild type GFP, non-UV excitation) | 475 | 509 | ![]() |
|
YFP | 512 | 529 | ![]() |
|
YO-PRO-1 | 491 | 507 | ![]() |
|
YOYO-1 | 491 | 509 | ![]() |
YFP (500/24 nm Excitation; 524/27 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Acridine Orange + DNA | 500 | 526 | ![]() |
|
EYFP | 514 | 527 | ![]() |
RFP (531/40 nm Excitation; 593/40 nm Emission)
Texas Red (585/29 nm Excitation; 624/40 nm Emission)
*See the complete list of CellLights Reagents
Cy5 (628/40 nm Excitation; 692/40 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 633 | 631 | 650 | ![]() |
|
Alexa Fluor 635 | 634 | 647 | ![]() |
|
Alexa Fluor 647 | 650 | 670 | ![]() |
|
Alexa Fluor 660 | 663 | 691 | ![]() |
|
Alexa Fluor 680 | 679 | 704 | ![]() |
|
APC (Allophycocyanin) | 651 | 660 | ![]() |
|
BODIPY 630/650X | 626 | 641 | ![]() |
|
BODIPY 650/665 | 647 | 665 | ![]() |
|
Carboxynaphthofluorescein | 600 | 674 | ![]() |
|
CellMask Deep Red plasma membrane stain | 658 | 676 | ![]() |
|
CellRox Deep Red | 640 | 665 | ![]() |
|
Click-iT Alexa Fluor 647 | 650 | 670 | ![]() |
|
Cy5 | 648 | 666 | ![]() |
|
Cy5.5 | 678 | 696 | ![]() |
|
DDAO | 648 | 658 | ![]() |
|
DiD (Vybrant DiD) | 645 | 670 | ![]() |
|
DRAQ5 | 650 | 680 | ![]() |
|
DyLight 633 | 638 | 658 | ![]() |
|
DyLight 649 | 654 | 673 | ![]() |
|
FluoSpheres crimson microspheres | 621 | 646 | ![]() |
|
FluoSpheres dark red microspheres | 657 | 683 | ![]() |
|
FxCycle Far Red | 641 | 657 | ![]() |
|
HCS CellMask Deep Red stain | 649 | 670 | ![]() |
|
HCS LipidTOX Deep Red | 634 | 652 | ![]() |
|
HCS NuclearMask Deep Red | 635 | 687 | ![]() |
|
HCS NuclearMask Red | 622 | 644 | ![]() |
|
LIVE/DEAD Fixable Far Red | 650 | 665 | ![]() |
|
LysoTracker Deep Red | 647 | 668 | ![]() |
|
MitoTracker Deep Red FM | 641 | 661 | ![]() |
|
NucRed Dead 647 | 642 | 661 | ![]() |
|
NucRed Live 647 | 638 | 686 | ![]() |
|
SYTO 17 | 619 | 638 | ![]() |
|
SYTO 59 | 621 | 644 | ![]() |
|
SYTO 60 | 649 | 681 | ![]() |
|
SYTO 62 | 650 | 681 | ![]() |
|
SYTOX Red | 640 | 658 | ![]() |
|
TO-PRO-3 | 642 | 657 | ![]() |
|
TOTO-3 | 642 | 661 | ![]() |
|
Tubulin Tracker Deep Red | 652 | 669 | ![]() |
|
Vybrant DyeCycle Ruby | 638 | 686 | ![]() |
Cy5.5 (655/46 nm Excitation; 794/16 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 660 | 663 | 691 | ![]() |
|
Alexa Fluor 680 | 679 | 702 | ![]() |
|
Alexa Fluor 700 | 696 | 719 | ![]() |
|
ATTO 635 | 635 | 658 | ![]() |
|
ATTO 647 | 644 | 670 | ![]() |
|
ATTO 655 | 663 | 683 | ![]() |
|
ATTO 680 | 680 | 700 | ![]() |
|
ATTO 700 | 699 | 719 | ![]() |
|
Cy5.5 | 672 | 690 | ![]() |
|
DY-675 | 675 | 699 | ![]() |
|
DyLight 680 | 676 | 705 | ![]() |
|
FluoSpheres Dark Red fluorescent microspheres | 656 | 683 | ![]() |
|
HiLyte Fluor 680 | 688 | 702 | ![]() |
|
IRDye 680 NHS Ester | 683 | 710 | ![]() |
|
IRDye 700 Phosphoramidite | 691 | 699 | ![]() |
|
Qdot 705 Nanocrystals | 300 | 702 | ![]() |
|
Quasa 705 | 688 | 706 | ![]() |
|
SYTO 60 | 650 | 681 | ![]() |
Cy7 (710/40 nm Excitation; 775/46 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
CellVue NIR780 | 743 | 776 | ![]() |
|
Cy7 | 745 | 766 | ![]() |
|
DyLight 750 | 752 | 778 | ![]() |
|
Indocyanine Green | 768 | 807 | ![]() |
|
IRDye 800CW | 774 | 789 | ![]() |
|
LIVE/DEAD Fixable near-IR | 750 | 775 | ![]() |
|
XenoLight CF770 | 770 | 797 | ![]() |
Note: Near Infrared dyes (e.g., IRDye 800CW) work best with low-magnification, high-NA objectives to maximize detection sensitivity.
CFP/YFP (445/45 nm Excitation; 542/27 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
CFP | 434 | 477 | Control YFP Emission |
Notes: CFP-YFP FRET control. CFP excitation coupled with a YFP emission filter. For FRET sensitized-emission donor-acceptor bleedthrough measurements. Requires CFP and YFP light cubes for CFP-YFP FRET experiments.
AO (442/46 nm Excitation; 488LP nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Acridine Orange + RNA | 460 | 650 | ![]() |
|
Acridine Orange + DNA | 500 | 526 | ![]() |
|
Alexa Fluor 430 | 434 | 540 | ![]() |
|
AmCyan | 548 | 489 | ![]() |
|
Auramine O | 460 | 550 | ![]() |
|
CTC Formazan | 450 | 630 | ![]() |
|
Di-8 ANEPPS | 468 | 635 | ![]() |
|
DiA (FAST DiA) | 491 | 613 | ![]() |
|
Lucifer Yellow | 428 | 544 | ![]() |
|
LysoSensor Green (pH 5) | 442 | 505 | ![]() |
|
PerCP | 488 | 675 | ![]() |
Notes: Acridine Orange, longpass emission. To visualize Acridine Orange in green and orange/red emission wavelengths, we recommend using this light cube with the EVOS FL color or EVOS FL Auto cell imaging systems. The AO cube passes simultaneous green, yellow, and red fluorescence, which requires color camera detection. Works well with other fluorophores listed below in either monochrome or color camera EVOS FL cell imaging systems, provided the sample is labeled with only one dye.
AOred (442/46 nm Excitation; 628/32 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Acridine Orange + RNA | 460 | 650 | ![]() |
|
Acridine Orange + DNA | 500 | 526 | ![]() |
|
CTC Formazan | 450 | 630 | ![]() |
|
Di-8 ANEPPS | 468 | 635 | ![]() |
|
DiA | 491 | 613 | ![]() |
|
Fura Red (high Ca2+) | 436 | 637 | ![]() |
|
Lucifer yellow | 428 | 544 | ![]() |
Notes: Acridine Orange, red emission. Can be used with either EVOS FL color or EVOS FL monochrome Cell Imaging Systems. Detects red fluorescence, which can be visualized with either a color or a monochrome camera.
*Poor spectral match. Use the GFP or YFP light cube instead.
Qdot 525 (445/45 nm Excitation; 525/15 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Acridine Orange (+DNA) | 500 | 526 | ![]() |
|
Alexa Fluor 430 | 432 | 540 | ![]() |
|
Alexa Fluor 488 | 496 | 518 | ![]() |
|
BCECF (pH 5.2) | 499 | 540 | ![]() |
|
BOBO-1 | 570 | 602 | ![]() |
|
Calcein | 494 | 540 | ![]() |
|
Carboxyfluorescein (5-FAM) | 492 | 518 | ![]() |
|
CellTracker Green CMFDA | 490 | 540 | ![]() |
|
CellTracker Violet BMQC | 415 | 516 | ![]() |
|
CFP | 434 | 540 | ![]() |
|
Cy2 | 492 | 540 | ![]() |
|
CyQUANT Direct | 500 | 540 | ![]() |
|
DAF-FM | 493 | 540 | ![]() |
|
DiA | 456 | 590 | ![]() |
|
DiO | 484 | 501 | ![]() |
|
F2N12S | 405 | 530, 585 | ![]() |
|
FITC | 492 | 520 | ![]() |
|
Fluo-4 | 494 | 540 | ![]() |
|
FM 1-43 | 510 | 626 | ![]() |
|
GFP | 488 | 507 | ![]() |
|
HCS CellMask Green | 493 | 516 | ![]() |
|
LIVE/DEAD Fixable Green | 498 | 506 | ![]() |
|
LIVE/DEAD Fixable Yellow | 405 | 575 | ![]() |
|
Lucifer Yellow | 423 | 543 | ![]() |
|
LysoSensor Green | 448 | 503 | ![]() |
|
LysoSensor Yellow/Blue (pH 8.0) | 329 | 440 | ![]() |
|
LysoTracker Green | 504 | 511 | ![]() |
|
MitoTracker Green FM | 490 | 512 | ![]() |
|
NBD-X | 467 | 538 | ![]() |
|
NeuroTrace 500/525 Nissl stain | 497 | 524 | ![]() |
|
Oregon Green 488 | 498 | 524 | ![]() |
|
Pacific Orange | 440 | 551 | ![]() |
|
POPO-1 | 434 | 456 | ![]() |
|
PO-PRO-1 | 435 | 455 | ![]() |
|
Qdot 525 | UV | 525 | ![]() |
|
Rhodamine 110 | 496 | 520 | ![]() |
|
Rhodamine Green | 497 | 523 | ![]() |
|
SYBR Gold | 495 | 539 | ![]() |
|
SYBR Green I | 497 | 521 | ![]() |
|
SYTO 9 | 482 | 500 | ![]() |
|
SYTO 13 | 487 | 505 | ![]() |
|
SYTO 16 | 490 | 520 | ![]() |
|
SYTO 45 | 452 | 484 | ![]() |
|
SYTO RNASelect | 503 | 527 | ![]() |
|
SYTOX Blue | 444 | 480 | ![]() |
|
ThiolTracker Violet | 405 | 526 | ![]() |
|
YO-PRO-1 | 491 | 507 | ![]() |
|
YOYO-1 | 491 | 509 | ![]() |
Qdot 545 (445/45 nm Excitation; 545/22 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 430 | 432 | 540 | ![]() |
|
BCECF (pH 5.2) | 499 | 540 | ![]() |
|
BOBO-1 | 570 | 602 | ![]() |
|
Calcein | 494 | 540 | ![]() |
|
CellTracker Green CMFDA | 490 | 540 | ![]() |
|
CellTracker Violet BMQC | 415 | 516 | ![]() |
|
CFP | 434 | 540 | ![]() |
|
Cy2 | 492 | 540 | ![]() |
|
CyQUANT Direct | 500 | 540 | ![]() |
|
DiA | 456 | 590 | ![]() |
|
DiO | 484 | 501 | ![]() |
|
F2N12S | 405 | 530, 585 | ![]() |
|
FITC | 492 | 520 | ![]() |
|
FM 1-43 | 510 | 626 | ![]() |
|
GFP | 488 | 507 | ![]() |
|
LIVE/DEAD Fixable Yellow | 405 | 575 | ![]() |
|
Lucifer Yellow | 423 | 543 | ![]() |
|
LysoSensor Green | 448 | 503 | ![]() |
|
LysoSensor Yellow/Blue (pH 8.0) | 329 | 440 | ![]() |
|
MitoTracker Green FM | 490 | 512 | ![]() |
|
NBD-X | 467 | 538 | ![]() |
|
NeuroTrace 500/525 Nissl stain | 497 | 524 | ![]() |
|
Oregon Green 488 | 498 | 524 | ![]() |
|
Pacific Orange | 440 | 551 | ![]() |
|
Qdot 545 | UV | 545 | ![]() |
|
Rhodamine 110 | 496 | 520 | ![]() |
|
Rhodamine Green | 497 | 523 | ![]() |
|
SYBR Gold | 495 | 539 | ![]() |
|
SYBR Green I | 497 | 521 | ![]() |
|
SYTO 9 | 482 | 500 | ![]() |
|
SYTO 13 | 487 | 505 | ![]() |
|
SYTO 16 | 490 | 520 | ![]() |
|
SYTO 45 | 452 | 484 | ![]() |
|
SYTO RNASelect | 503 | 527 | ![]() |
|
ThiolTracker Violet | 405 | 526 | ![]() |
|
YO-PRO-1 | 491 | 507 | ![]() |
|
YOYO-1 | 491 | 509 | ![]() |
Qdot 565 (445/45 nm Excitation; 565/16 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 430 | 432 | 540 | ![]() |
|
BCECF (pH 5.2) | 499 | 540 | ![]() |
|
CellTracker Violet BMQC | 415 | 516 | ![]() |
|
CFP | 434 | 540 | ![]() |
|
Cy2 | 492 | 540 | ![]() |
|
Di-8-ANEPPS | 465 | 635 | ![]() |
|
DiA | 456 | 590 | ![]() |
|
F2N12S | 405 | 530, 585 | ![]() |
|
FM 1-43 | 510 | 626 | ![]() |
|
LIVE/DEAD Fixable Yellow | 405 | 575 | ![]() |
|
Lucifer Yellow | 423 | 543 | ![]() |
|
LysoSensor Green | 448 | 503 | ![]() |
|
LysoSensor Yellow/Blue (pH 8.0) | 329 | 440 | ![]() |
|
NBD-X | 467 | 538 | ![]() |
|
NeuroTrace 500/525 Nissl stain | 497 | 524 | ![]() |
|
Pacific Orange | 440 | 551 | ![]() |
|
Qdot 565 | UV | 565 | ![]() |
|
SYBR Gold | 495 | 539 | ![]() |
|
SYBR Green I | 497 | 521 | ![]() |
|
SYTO 9 | 482 | 500 | ![]() |
|
SYTO 13 | 487 | 505 | ![]() |
|
SYTO 16 | 490 | 520 | ![]() |
|
SYTO 45 | 452 | 484 | ![]() |
|
SYTO RNASelect | 503 | 527 | ![]() |
|
ThiolTracker Violet | 405 | 526 | ![]() |
|
YOYO-1 | 491 | 509 | ![]() |
Qdot 585 (445/45 nm Excitation; 586/20 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 430 | 432 | 540 | ![]() |
|
BCECF (pH 5.2) | 499 | 540 | ![]() |
|
CellTracker Violet BMQC | 415 | 516 | ![]() |
|
Di-8-ANEPPS | 465 | 635 | ![]() |
|
DiA | 456 | 590 | ![]() |
|
F2N12S | 405 | 530, 585 | ![]() |
|
FM 1-43 | 510 | 626 | ![]() |
|
Fura Red (+Ca2+) | 436 | 655 | ![]() |
|
LIVE/DEAD Fixable Yellow | 405 | 575 | ![]() |
|
Lucifer Yellow | 423 | 543 | ![]() |
|
NBD-X | 467 | 538 | ![]() |
|
Nile red (triglyceride) | 552 | 636 | ![]() |
|
Pacific Orange | 440 | 551 | ![]() |
|
Qdot 585 | UV | 585 | ![]() |
|
SYPRO Ruby | 450 | 618 | ![]() |
|
ThiolTracker Violet | 405 | 526 | ![]() |
Qdot 605 (445/45 nm Excitation; 505/15 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 430 | 432 | 540 | ![]() |
|
BCECF (pH 5.2) | 499 | 540 | ![]() |
|
Di-8-ANEPPS | 465 | 635 | ![]() |
|
DiA | 456 | 590 | ![]() |
|
F2N12S | 405 | 530, 585 | ![]() |
|
FM 1-43 | 510 | 626 | ![]() |
|
Fura Red (+Ca2+) | 436 | 655 | ![]() |
|
Fura Red (-Ca2+) | 473 | 670 | ![]() |
|
LIVE/DEAD Fixable Yellow | 405 | 575 | ![]() |
|
Lucifer Yellow | 423 | 543 | ![]() |
|
NBD-X | 467 | 538 | ![]() |
|
Nile red (triglyceride) | 552 | 636 | ![]() |
|
Pacific Orange | 440 | 551 | ![]() |
|
Qdot 605 | UV | 605 | ![]() |
|
RH 795 | 530 | 712 | ![]() |
|
SYPRO Ruby | 450 | 618 | ![]() |
Qdot 625 (445/45 nm Excitation; 625/15 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Di-8-ANEPPS | 465 | 635 | ![]() |
|
DiA | 456 | 590 | ![]() |
|
F2N12S | 405 | 530, 585 | ![]() |
|
FM 1-43 | 510 | 626 | ![]() |
|
Fura Red (+Ca2+) | 436 | 655 | ![]() |
|
Fura Red (-Ca2+) | 473 | 670 | ![]() |
|
Lucifer Yellow | 423 | 543 | ![]() |
|
Qdot 625 | UV | 625 | ![]() |
|
RH 795 | 530 | 712 | ![]() |
|
SYPRO Ruby | 450 | 618 | ![]() |
Qdot 655 (445/45 nm Excitation; 655/15 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Acridine Orange (+RNA) | 460 | 650 | ![]() |
|
Di-8-ANEPPS | 465 | 635 | ![]() |
|
DiA | 456 | 590 | ![]() |
|
FM 1-43 | 510 | 626 | ![]() |
|
FM 4-64 | 558 | 734 | ![]() |
|
Fura Red (+Ca2+) | 436 | 655 | ![]() |
|
Fura Red (-Ca2+) | 473 | 670 | ![]() |
|
Qdot 655 | UV | 655 | ![]() |
|
RH 795 | 530 | 712 | ![]() |
|
SYPRO Ruby | 450 | 618 | ![]() |
Qdot 705 (445/45 nm Excitation; 705/40 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Di-8-ANEPPS | 465 | 635 | ![]() |
|
FM 4-64 | 558 | 734 | ![]() |
|
Fura Red (+Ca2+) | 436 | 655 | ![]() |
|
Fura Red (-Ca2+) | 473 | 670 | ![]() |
|
Qdot 705 | UV | 705 | ![]() |
|
RH 795 | 530 | 712 | ![]() |
|
SYPRO Ruby | 450 | 618 | ![]() |
Qdot 800 (445/45 nm Excitation; 785/62 nm Emission)
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
FM 4-64 | 558 | 734 | ![]() |
|
Qdot 800 | UV | 800 | ![]() |