Invitrogen™
CellMask™ Orange Actin Tracking Stain
Description
Invitrogen CellMask Orange Actin Tracking Stain fluorescently labels polymerized/filamentous actin (F-actin) in live or fixed cells. It is designed to readily permeate live cells, thus providing more uniform and bright labeling. CellMask Orange Actin Tracking Stain can be used to track F-actin in live cells for 24 hours or more. Live cells stained with CellMask Orange Actin Tracking Stain can be fixed, or it can be used with fixed cells. And the stain can be used for multiplexing with antibodies in immunofluorecence (IF), immunocytochemistry (ICC), and immunohistochemistry (IHC) protocols.Features of Cell Mask Orange Actin Tracking Stain include:
• Stains F-actin in live or fixed cells for fluorescence imaging
• Can be multiplexed with antibodies in IF/ICC/IHC experiments
• Fluorescence maxima: Ex 545 nm/Em 570 nm; can be detected with TRITC/RFP filter
Selection guide for cellular cytoskeleton staining probes
CellMask actin tracking stains are available in three colors: green (Cat. No. A57246) detected with a FITC/GFP filter (Ex 503 nm/Em 512 nm), orange (Cat. No. A57247) detected with a TRITC/RFP filter (Ex 545 nm/Em 570 nm), and red (Cat. No. A57248) detected with a CY5/Deep Red filter (Ex 652 nm/Em 669 nm). Excitation and emission spectra of all three stains are provided below.
CellMask actin tracking stains detect F-actin with a targeting structure that closely resembles jasplakinolide. They do not label globular actin (G-actin) or monomer actin (M-actin). CellMask actin tracking stains easily pass through live cell membranes and are retained within live cells after loading. As demonstrated in the figures below, incubation with CellMask actin tracking stains has no detectable effect on viability of various cell lines after 24 hours of incubation.
CellMask actin tracking stains detect F-actin in live cells or tissue, which can then be fixed. In addition, CellMask actin tracking stains can be used to detect F-Actin in paraformaldehyde fixed cells or tissue (freshly fixed or cryo frozen only) and can be multiplexed with antibody detection. CellMask actin tracking stains can also be used in 3D cell culture for 3D imaging.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Product Type: | Reagent |
|---|---|
| Target: | F-Actin |
| Emission: | 570 nm |
| Excitation: | 545 nm |
| Excitation/Emission: | 545 nm/Em 570 nm |
| Filter Type: | TRITC/RFP |
| Flow Cytometer Laser Lines: | Green (532 nm) or Yellow (561-568 nm) |
| Shipping Condition: | Room Temperature |
| Sub Cellular Localization: | Actin Cytoskeleton |
| Product Line: | Molecular Probes™ |
| Detection Method: | Fluorescence Microscopy |
| For Use With (Equipment): | Fluorescence Microscope, or High Content Analyzer |
Contents & storage
Contains 1 vial with enough reagent to stain 120 slides, when 500 µL of 1X staining solution is used per slide.
Add 60 µL/vial of anhydrous DMSO to make a 1000X (1mM) stock solution. Dissolve stock solution 1:1000 in cell imaging solution to make 1X (1 µM) staining solution.
Store at room temperature. Can be stored to -20°C with no freeze/thaw cycles.
When stored as instructed, these reagents are effective for a minimum of 6 months after receiving.
Add 60 µL/vial of anhydrous DMSO to make a 1000X (1mM) stock solution. Dissolve stock solution 1:1000 in cell imaging solution to make 1X (1 µM) staining solution.
Store at room temperature. Can be stored to -20°C with no freeze/thaw cycles.
When stored as instructed, these reagents are effective for a minimum of 6 months after receiving.
