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Zitierungen und Referenzen (72)
Invitrogen™
ER-Tracker™ Blue-White DPX, für die Bildgebung lebender Zellen
Der ER-Tracker Blue-White DPX Farbstoff ist eine photostabile Sonde, die selektiv für das endoplasmatische Retikulum (ER) in lebenden Zellen ist.Weitere Informationen
| Katalognummer | Menge |
|---|---|
| E12353 | 20 x 50 μL |
Katalognummer E12353
Preis (EUR)
651,65
Exklusiv online
724,00Ersparnis 72,35 (10%)
Each
Menge:
20 x 50 μL
Preis (EUR)
651,65
Exklusiv online
724,00Ersparnis 72,35 (10%)
Each
Der ER-Tracker Blue-White DPX Farbstoff ist eine photostabile Sonde, die selektiv für das endoplasmatische Retikulum (ER) in lebenden Zellen ist. Die Zellen können nach dem Färben mit dem Farbstoff aldehyd-fixiert werden, dabei geht jedoch merksam Fluoreszenzstärke verloren. Bei Anregung bei ∼374 nm weist die umweltempfindliche Sonde Emissionen zwischen 430 und 640 nm auf. Ein Long-Pass DAPI Filter funktioniert am besten.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
FarbeBlau, Blau
Konzentration1 mM
NachweisverfahrenFluoreszent, Fluoreszent
Zur Verwendung mit (Geräte)Fluoreszenzmikroskop, Fluoreszenzmikroskop
ProduktlinieER-Tracker
Menge20 x 50 μL
VersandbedingungRaumtemperatur, Raumtemperatur
MarkertypFluorescent Dye
ProdukttypFarbstoff
SubCellular LocalizationEndoplasmatisches Retikulum
Unit SizeEach
Inhalt und Lagerung
Bei -5 bis -30 °C lagern und vor Licht schützen.
Zitierungen und Referenzen (72)
Zitierungen und Referenzen
Abstract
CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.
Journal:J Biol Chem
PubMed ID:17215251
'Chlamydia trachomatis is an obligate intracellular pathogen that can persist in the urogenital tract. Mechanisms by which C. trachomatis evades clearance by host innate immune responses are poorly described. CD1d is MHC-like, is expressed by epithelial cells, and can signal innate immune responses by NK and NKT cells. Here we
Microtubules regulate local Ca2+ spiking in secretory epithelial cells.
Journal:J Biol Chem
PubMed ID:10801885
'The role of the cytoskeleton in regulating Ca(2+) release has been explored in epithelial cells. Trains of local Ca(2+) spikes were elicited in pancreatic acinar cells by infusion of inositol trisphosphate through a whole cell patch pipette, and the Ca(2+)-dependent Cl(-) current spikes were recorded. The spikes were only transiently
Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
Journal:Histochem Cell Biol
PubMed ID:17849139
'Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored
TLR9 signals after translocating from the ER to CpG DNA in the lysosome.
Journal:Nat Immunol
PubMed ID:14716310
'Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is activated. We show
Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
Journal:J Microsc
PubMed ID:12423261
'Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle