CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.
AuthorsKawana K, Quayle AJ, Ficarra M, Ibana JA, Shen L, Kawana Y, Yang H, Marrero L, Yavagal S, Greene SJ, Zhang YX, Pyles RB, Blumberg RS, Schust DJ
JournalJ Biol Chem
PubMed ID17215251
'Chlamydia trachomatis is an obligate intracellular pathogen that can persist in the urogenital tract. Mechanisms by which C. trachomatis evades clearance by host innate immune responses are poorly described. CD1d is MHC-like, is expressed by epithelial cells, and can signal innate immune responses by NK and NKT cells. Here we ... More
Microtubules regulate local Ca2+ spiking in secretory epithelial cells.
AuthorsFogarty KE, Kidd JF, Turner A, Skepper JN, Carmichael J, Thorn P
JournalJ Biol Chem
PubMed ID10801885
'The role of the cytoskeleton in regulating Ca(2+) release has been explored in epithelial cells. Trains of local Ca(2+) spikes were elicited in pancreatic acinar cells by infusion of inositol trisphosphate through a whole cell patch pipette, and the Ca(2+)-dependent Cl(-) current spikes were recorded. The spikes were only transiently ... More
Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
AuthorsSoldani C, Croce AC, Bottone MG, Fraschini A, Biggiogera M, Bottiroli G, Pellicciari C,
JournalHistochem Cell Biol
PubMed ID17849139
'Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored ... More
TLR9 signals after translocating from the ER to CpG DNA in the lysosome.
AuthorsLatz E, Schoenemeyer A, Visintin A, Fitzgerald KA, Monks BG, Knetter CF, Lien E, Nilsen NJ, Espevik T, Golenbock DT
JournalNat Immunol
PubMed ID14716310
'Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is activated. We show ... More
Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
AuthorsBestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H
JournalJ Microsc
PubMed ID12423261
'Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle ... More
Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis.
AuthorsMarijanovic Z, Laubner D, Moller G, Gege C, Husen B, Adamski J, Breitling R
JournalMol Endocrinol
PubMed ID12829805
'The protein encoded by the HSD17B7 gene was originally described as a prolactin receptor-associated protein and as 17beta-hydroxysteroid dehydrogenase (HSD) type 7. Its ability to synthesize 17beta-estradiol in vitro has been reported previously. However, we demonstrate that HSD17B7 is the ortholog of the yeast 3-ketosteroid reductase Erg27p and converts zymosterone ... More
Mitochondrial Ca2+ uptake regulates the excitability of myenteric neurons.
AuthorsVanden Berghe P, Kenyon JL, Smith TK
JournalJ Neurosci
PubMed ID12177194
'We investigated the role of mitochondria in the regulation of intracellular Ca2+ ([Ca2+]i) and excitability of myenteric neurons in guinea pig ileum, using microelectrodes and fura-2 [Ca2+]i measurements. In AH/Type-II neurons, action potentials evoke ryanodine-sensitive increases in [Ca2+]i that activate Ca2+-dependent K+ channels and slow afterhyperpolarizations (AH) lasting approximately 15 ... More
Vanilloid receptor 1 regulates multiple calcium compartments and contributes to Ca2+-induced Ca2+ release in sensory neurons.
AuthorsKárai LJ, Russell JT, Iadarola MJ, Oláh Z
JournalJ Biol Chem
PubMed ID14963041
'Vanilloid receptor 1 belongs to the transient receptor potential ion channel family and transduces sensations of noxious heat and inflammatory hyperalgesia in nociceptive neurons. These neurons contain two vanilloid receptor pools, one in the plasma membrane and the other in the endoplasmic reticulum. The present experiments characterize these two pools ... More
Paralytic zebrafish lacking acetylcholine receptors fail to localize rapsyn clusters to the synapse.
AuthorsOno F, Higashijima S, Shcherbatko A, Fetcho JR, Brehm P
JournalJ Neurosci
PubMed ID11466415
'Physiological analysis of two lines of paralytic mutant zebrafish, relaxed and sofa potato, reveals defects in distinct types of receptors in skeletal muscle. In sofa potato the paralysis results from failed synaptic transmission because of the absence of acetylcholine receptors, whereas relaxed mutants lack dihydropyridine receptor-mediated release of internal calcium ... More
Versatile regulation of cytosolic Ca2+ by vanilloid receptor I in rat dorsal root ganglion neurons.
AuthorsLiu M, Liu MC, Magoulas C, Priestley JV, Willmott NJ
JournalJ Biol Chem
PubMed ID12454015
'Analysis of small dorsal root ganglion (DRG) neurons revealed novel functions for vanilloid receptor 1 (VR1) in the regulation of cytosolic Ca(2+). The VR1 agonist capsaicin induced Ca(2+) mobilization from intracellular stores in the absence of extracellular Ca(2+), and this release was inhibited by the VR1 antagonist capsazepine but was ... More
Critical upstream signals of cytochrome C release induced by a novel Bcl-2 inhibitor.
AuthorsAn J, Chen Y, Huang Z
JournalJ Biol Chem
PubMed ID14966123
'Cytochrome c release is a central step in the apoptosis induced by many death stimuli. Bcl-2 plays a critical role in controlling this step. In this study, we investigated the upstream mechanism of cytochrome c release induced by ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (HA14-1), a recently discovered small molecule inhibitor of Bcl-2. HA14-1 ... More
Messenger-specific role for nicotinic acid adenine dinucleotide phosphate in neuronal differentiation.
AuthorsBrailoiu E, Churamani D, Pandey V, Brailoiu GC, Tuluc F, Patel S, Dun NJ
JournalJ Biol Chem
PubMed ID16595650
'Cells possess several Ca2+-mobilizing messengers, which couple stimulation at the cell surface by a multitude of extracellular cues to the regulation of intracellular Ca2+-sensitive targets. Recent studies suggest that agonists differentially select from this molecular palette to generate their characteristic Ca2+ signals but it is still unclear whether different messengers ... More
Visualizing formation and dynamics of vacuoles in living cells using contrasting dextran-bound indicator: endocytic and nonendocytic vacuoles.
'Here we describe a technique that allows us to visualize in real time the formation and dynamics (fusion, changes of shape, and translocation) of vacuoles in living cells. The technique involves infusion of a dextran-bound fluorescent probe into the cytosol of the cell via a patch pipette, using the whole-cell ... More
Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.
'Amelogenin is an extracellular matrix protein secreted by ameloblasts and is a major component of enamel matrix. Recently, in addition to their role in enamel formation, the biological activity of enamel proteins in the process of cell differentiation has recently become widely appreciated. In this study, we examined the biological ... More
Truncation of the N-terminal ectodomain has implications in the N-glycosylation and transport to the cell surface of Edg-1/S1P1 receptor.
AuthorsKohno T, Igarashi Y
JournalJ Biochem (Tokyo)
PubMed ID14688232
'The endothelial cell-expressed sphingosine 1-phosphate receptors Edg-1/S1P1 and Edg-3/S1P3 have been implicated in various physiological events such as the regulation of angiogenesis. Since there is an excess of a ligand constitutively in blood, these receptors may have some mechanism(s) avoiding overstimulation. In this study, we found that the N-terminal ectodomains ... More
Depletion of calcium stores in injured sensory neurons: anatomic and functional correlates.
AuthorsGemes G, Rigaud M, Weyker PD, Abram SE, Weihrauch D, Poroli M, Zoga V, Hogan QH,
JournalAnesthesiology
PubMed ID19602957
'BACKGROUND: Painful nerve injury leads to disrupted Ca signaling in primary sensory neurons, including decreased endoplasmic reticulum (ER) Ca storage. This study examines potential causes and functional consequences of Ca store limitation after injury. METHODS: Neurons were dissociated from axotomized fifth lumbar (L5) and the adjacent L4 dorsal root ganglia ... More
Brefeldin A affects growth, endoplasmic reticulum, Golgi bodies, tubular vacuole system, and secretory pathway in Pisolithus tinctorius.
AuthorsCole L, Davies D, Hyde GJ, Ashford AE
JournalFungal Genet Biol
PubMed ID10919378
'Brefeldin A (BFA) reduced radial growth in Pisolithus tinctorius at a concentration as low as 2 microM. Use of endoplasmic reticulum (ER)-Tracker dye, unconjugated BFA, and fluorescent BFA (BODIPY-BFA) allowed comparison of the effects of BFA on the endomembrane system of P. tinctorius at the light and electron microscope levels. ... More
Dynein supports motility of endoplasmic reticulum in the fungus Ustilago maydis.
AuthorsWedlich-Söldner R, Schulz I, Straube A, Steinberg G
JournalMol Biol Cell
PubMed ID11907275
'The endoplasmic reticulum (ER) of most vertebrate cells is spread out by kinesin-dependent transport along microtubules, whereas studies in Saccharomyces cerevisiae indicated that motility of fungal ER is an actin-based process. However, microtubules are of minor importance for organelle transport in yeast, but they are crucial for intracellular transport within ... More
Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons.
AuthorsBannai H, Inoue T, Nakayama T, Hattori M, Mikoshiba K
JournalJ Cell Sci
PubMed ID14676272
'Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal ... More
Differential partitioning of maternal fatty acid and phospholipid in neonate mosquito larvae.
AuthorsAtella GC, Shahabuddin M
JournalJ Exp Biol
PubMed ID12409488
'In animals, lipids are a source of energy, cell membrane components, signaling pathway modulators and emulsifying agents. In egg-laying animals, maternal yolk lipids, imported into the egg before laying, are metabolized or distributed in the developing embryo to serve these functions. Studies with birds, reptiles and insects have described lipid ... More
Ca(2+) dynamics in the lumen of the endoplasmic reticulum in sensory neurons: direct visualization of Ca(2+)-induced Ca(2+) release triggered by physiological Ca(2+) entry.
AuthorsSolovyova N, Veselovsky N, Toescu EC, Verkhratsky A
JournalEMBO J
PubMed ID11847110
'In cultured rat dorsal root ganglia neurons, we measured membrane currents, using the patch-clamp whole-cell technique, and the concentrations of free Ca(2+) in the cytosol ([Ca(2+)](i)) and in the lumen of the endoplasmic reticulum (ER) ([Ca(2+)](L)), using high- (Fluo-3) and low- (Mag-Fura-2) affinity Ca(2+)-sensitive fluorescent probes and video imaging. Resting ... More
Cloning and characterization of N4WBP5A, an inducible, cyclosporine-sensitive, Nedd4-binding protein in human T lymphocytes.
AuthorsCristillo AD, Nie L, Macri MJ, Bierer BE,
JournalJ Biol Chem
PubMed ID12796489
'We have cloned and characterized a human cDNA, designated N4WBP5A, that belongs to the family of Nedd4-binding proteins. We originally identified N4WBP5A as an unknown expressed sequence tag (AA770150) represented in a cDNA microarray analysis that was up-regulated upon activation of T cells and inhibited by cell treatment with the ... More
In vitro and in vivo photocytotoxicity of boron dipyrromethene derivatives for photodynamic therapy.
AuthorsLim SH, Thivierge C, Nowak-Sliwinska P, Han J, van den Bergh H, Wagnières G, Burgess K, Lee HB,
JournalJ Med Chem
PubMed ID20199028
To understand the effects of substitution patterns on photosensitizing the ability of boron dipyrromethene (BODIPY), two structural variations that either investigate the effectiveness of various iodinated derivatives to maximize the ... More
Essential role of MD-2 in LPS responsiveness and TLR4 distribution.
AuthorsNagai Y, Akashi S, Nagafuku M, Ogata M, Iwakura Y, Akira S, Kitamura T, Kosugi A, Kimoto M, Miyake K
JournalNat Immunol
PubMed ID12055629
Toll-like receptor 4 (TLR4) mediates lipopolysaccharide (LPS) signaling in a variety of cell types. MD-2 is associated with the extracellular domain of TLR4 and augments TLR4-dependent LPS responses in vitro. We show here that MD-2(-/-) mice do not respond to LPS, do survive endotoxic shock but are susceptible to Salmonella ... More
The binding of VIP36 and alpha-amylase in the secretory vesicles via high-mannose type glycans.
AuthorsHara-Kuge S, Seko A, Shimada O, Tosaka-Shimada H, Yamashita K
JournalGlycobiology
PubMed ID15070860
Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to secretory vesicles in the glandula parotis of the ... More
Central role of mitofusin 2 in autophagosome-lysosome fusion in cardiomyocytes.
AuthorsZhao T, Huang X, Han L, Wang X, Cheng H, Zhao Y, Chen Q, Chen J, Cheng H, Xiao R, Zheng M,
JournalJ Biol Chem
PubMed ID22619176
In the heart, autophagy has been implicated in cardioprotection and ischemia-reperfusion tolerance, and the dysregulation of autophagy is associated with the development of heart failure. Mitochondrial dynamic proteins are profoundly involved in autophagic processes, especially the initiation and formation of autophagosomes, but it is not clear whether they play any ... More
Photoinduced cytotoxicity and biodistribution of prostate cancer cell-targeted porphyrins.
AuthorsSehgal I, Sibrian-Vazquez M, Vicente MG,
JournalJ Med Chem
PubMed ID18839477
A series of five porphyrin-peptide conjugates bearing one or two sequences containing a cell penetrating peptide (CPP), a nuclear localization signal (NLS), or a bifunctional CPP-NLS or NLS-CPP sequences were synthesized and investigated in vitro using PC-3M human prostate cancer cells, in comparison with FDA-approved purified hematoporphyrin derivative (Porfimer Sodium) ... More
Dynamics and mechanisms of quantum dot nanoparticle cellular uptake.
AuthorsXiao Y, Forry SP, Gao X, Holbrook RD, Telford WG, Tona A,
JournalJ Nanobiotechnology
PubMed ID20550705
ABSTRACT: BACKGROUND: The rapid growth of the nanotechnology industry and the wide application of various nanomaterials have raised concerns over their impact on the environment and human health. Yet little is known about the mechanism of cellular uptake and cytotoxicity of nanoparticles. An array of nanomaterials has recently been introduced ... More
Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis.
AuthorsInuzuka M, Hayakawa M, Ingi T,
JournalJ Biol Chem
PubMed ID16120614
Cell membranes contain various transporter proteins, some of which are responsible for transferring amino acids across membrane. In this study, we report another class of carrier proteins, termed Serinc1-5, that incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids. Serinc ... More
Diverse subcellular locations of cryptogein-induced reactive oxygen species production in tobacco Bright Yellow-2 cells.
AuthorsAshtamker C, Kiss V, Sagi M, Davydov O, Fluhr R,
JournalPlant Physiol
PubMed ID17277088
Reactive oxygen species (ROS) play a crucial role in many cellular responses and signaling pathways, including the oxidative burst defense response to pathogens. We have examined very early events in cryptogein-induced ROS production in tobacco (Nicotiana tabacum) Bright Yellow-2 suspension cells. Using Amplex Red and Amplex Ultra Red reagents, which ... More
A contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.
AuthorsAbodeely M, DuBois KN, Hehl A, Stefanic S, Sajid M, DeSouza W, Attias M, Engel JC, Hsieh I, Fetter RD, McKerrow JH,
JournalEukaryot Cell
PubMed ID19749174
The dynamic evolution of organelle compartmentalization in eukaryotes and how strictly compartmentalization is maintained are matters of ongoing debate. While the endoplasmic reticulum (ER) is classically envisioned as the site of protein cotranslational translocation, it has recently been proposed to have pluripotent functions. Using transfected reporter constructs, organelle-specific markers, and ... More
ER-Tracker dye and BODIPY-brefeldin A differentiate the endoplasmic reticulum and golgi bodies from the tubular-vacuole system in living hyphae of Pisolithus tinctorius.
AuthorsCole L, Davies D, Hyde GJ, Ashford AE
JournalJ Microsc
PubMed ID10692127
Two fluorochromes, ER-TrackerTM Blue-White DPX dye and the fluorescent brefeldin A (BFA) derivative, BODIPY-BFA, label the endoplasmic reticulum (ER) in hyphal tips of Pisolithus tinctorius and allow its differentiation from the tubular-vacuole system at the light microscope level in living cells. The ER-Tracker dye labels a reticulate network similar in ... More
An immunohistochemical method for the detection of proteins in the vertebrate lens.
AuthorsReed NA, Oh DJ, Czymmek KJ, Duncan MK
JournalJ Immunol Methods
PubMed ID11384685
Fluorescence immunohistochemistry has traditionally been difficult or impossible to perform on the vertebrate lens because of its extremely high protein content. Described here is a robust and rapid method for preparing and labeling vertebrate eyes for confocal microscopy. This technique has successfully been applied to localize proteins in the lens ... More
ZNRF proteins constitute a family of presynaptic E3 ubiquitin ligases.
AuthorsAraki T, Milbrandt J
JournalJ Neurosci
PubMed ID14561866
Protein ubiquitination has been implicated recently in neural development, plasticity, and degeneration. We previously identified ZNRF1/nin283, a protein with a unique, evolutionarily conserved C-terminal domain containing a juxtaposed zinc finger/RING finger combination. Here we describe the identification of a closely related protein, ZNRF2, thus defining a novel family of ZNRF ... More
Cancer promoted by the oncoprotein v-ErbA may be due to subcellular mislocalization of nuclear receptors.
AuthorsBonamy GM, Guiochon-Mantel A, Allison LA
JournalMol Endocrinol
PubMed ID15650025
The retroviral v-ErbA oncoprotein is a highly mutated variant of the thyroid hormone receptor alpha (TRalpha), which is unable to bind T(3) and interferes with the action of TRalpha in mammalian and avian cancer cells. v-ErbA dominant-negative activity is attributed to competition with TRalpha for T(3)-responsive DNA elements and/or auxiliary ... More
Spike, a novel BH3-only protein, regulates apoptosis at the endoplasmic reticulum.
AuthorsMund T, Gewies A, Schoenfeld N, Bauer MK, Grimm S
JournalFASEB J
PubMed ID12594175
We have isolated Spike, a novel and evolutionary conserved BH3-only protein. BH3-only proteins constitute a family of apoptosis inducers that mediate proapoptotic signals. In contrast to most proteins of this family, Spike was not found to be associated with mitochondria. Furthermore, unlike the known BH3-only proteins, Spike could not interact ... More
Inhibition of nuclear import and cell-cycle progression by mutated forms of the dynamin-like GTPase MxB.
AuthorsKing MC, Raposo G, Lemmon MA
JournalProc Natl Acad Sci U S A
PubMed ID15184662
Mx proteins form a subfamily of the dynamin-like GTPases, which have well established roles in cellular trafficking. Some Mx proteins (e.g., human MxA) have antiviral activity and are tightly regulated by type I IFNs. Others (e.g., human MxB) lack antiviral activity and are thought to have normal cellular functions that ... More
Reduction of detyrosinated microtubules and Golgi fragmentation are linked to tau-induced degeneration in astrocytes.
AuthorsYoshiyama Y, Zhang B, Bruce J, Trojanowski JQ, Lee VM
JournalJ Neurosci
PubMed ID14627651
Several human neurodegenerative diseases are associated with abnormal accumulations of aggregated tau proteins and glial degeneration in astrocytes, but the mechanism whereby tau proteins cause astrocytic degeneration is unclear. Here, we analyzed the biological consequences of overexpressing the longest human tau isoform in primary cultures of rat astrocytes using adenoviral-mediated ... More
Spectral shift of fluorescent dye FM4-64 reveals distinct microenvironment of nuclear envelope in living cells.
AuthorsZal T, Zal MA, Lotz C, Goergen CJ, Gascoigne NR
JournalTraffic
PubMed ID17052249
We report a distinct microenvironment within the nuclear envelope (NE) in living cells revealed by a spectral shift of the fluorescent dye FM4-64 (N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenylhexatrienyl)-pyridinium 2Br). The dye readily translocated to the NE at physiological temperature where it exhibited enhanced fluorescence when excited at 620-650 nm in contrast to 480-520 nm ... More
Involvement of caspase-4 in endoplasmic reticulum stress-induced apoptosis and Abeta-induced cell death.
AuthorsHitomi J, Katayama T, Eguchi Y, Kudo T, Taniguchi M, Koyama Y, Manabe T, Yamagishi S, Bando Y, Imaizumi K, Tsujimoto Y, Tohyama M
JournalJ Cell Biol
PubMed ID15123740
Recent studies have suggested that neuronal death in Alzheimer's disease or ischemia could arise from dysfunction of the endoplasmic reticulum (ER). Although caspase-12 has been implicated in ER stress-induced apoptosis and amyloid-beta (Abeta)-induced apoptosis in rodents, it is controversial whether similar mechanisms operate in humans. We found that human caspase-4, ... More
Independent signals determine the subcellular localization of NEP in prostate cancer cells.
AuthorsGomes I, Aumüller G, Wennemuth G, Bette M, Albrecht M
JournalBiochem Biophys Res Commun
PubMed ID14550292
NEP (Neutral endopeptidase 24.11) is a cell surface enzyme that hydrolyzes bioactive neuropeptides implicated in the transition from androgen-dependent prostate cancer (PC) to androgen-independent PC. We report the cloning and sequence analyses of NEP cDNAs from human androgen-responsive LNCaP PC cells and prostatic stromal cells. To investigate the functional role ... More
Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum.
AuthorsEchevarría W, Leite MF, Guerra MT, Zipfel WR, Nathanson MH
JournalNat Cell Biol
PubMed ID12717445
Calcium is a second messenger in virtually all cells and tissues. Calcium signals in the nucleus have effects on gene transcription and cell growth that are distinct from those of cytosolic calcium signals; however, it is unknown how nuclear calcium signals are regulated. Here we identify a reticular network of ... More
Homogeneous Ca2+ stores in rat adrenal chromaffin cells.
AuthorsInoue M, Sakamoto Y, Fujishiro N, Imanaga I, Ozaki S, Prestwich GD, Warashina A
JournalCell Calcium
PubMed ID12526884
The localization and function of Ca(2+) stores in isolated chromaffin cells of rat adrenal medulla were investigated using confocal laser microscopy and amperometry. Binding sites for BODIPY-inositol 1,4,5-trisphosphate (IP(3)), -ryanodine (Ry), and -thapsigargin (Thap) were both perinuclear and at the cell periphery. The endoplasmic reticulum (ER), which was identified by ... More
Placental extravillous cytotrophoblasts persistently express class I major histocompatibility complex molecules after human cytomegalovirus infection.
AuthorsTerauchi M, Koi H, Hayano C, Toyama-Sorimachi N, Karasuyama H, Yamanashi Y, Aso T, Shirakata M
JournalJ Virol
PubMed ID12857887
Human cytomegalovirus (HCMV) downregulates the class I major histocompatibility complexes (MHCs), HLA-A and -B, in infected fibroblasts to escape from antigen-specific cytotoxic T lymphocytes. The HCMV genes responsible for the downregulation of MHCs are US2, US3, US6, and US11, which encode type I membrane proteins working at the endoplasmic reticulum ... More
Identification of the expressed form of human cytosolic phospholipase A2beta (cPLA2beta): cPLA2beta3 is a novel variant localized to mitochondria and early endosomes.
AuthorsGhosh M, Loper R, Gelb MH, Leslie CC
JournalJ Biol Chem
PubMed ID16617059
In this study, we identify the principal splice variant of human cytosolic phospholipase A(2)beta (cPLA(2)beta) (also known as Group IVB cPLA(2)) present in cells. In human lung, spleen, and ovary and in a lung epithelial cell line (BEAS-2B), cPLA(2)beta is expressed as a 100-kDa protein, not the 114-kDa form originally ... More
Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology.
AuthorsStojanovski D, Koutsopoulos OS, Okamoto K, Ryan MT
JournalJ Cell Sci
PubMed ID14996942
Mitochondria undergo balanced fission and fusion events that enable their appropriate networking within the cell. In yeast, three factors have been identified that co-ordinate fission events at the mitochondrial outer membrane. Fis1p acts as the outer membrane receptor for recruitment of the dynamin member, Dnm1p and the WD40-repeat-containing protein Mdv1p. ... More
Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase.
AuthorsGuo K, Lukacik P, Papagrigoriou E, Meier M, Lee WH, Adamski J, Oppermann U
JournalJ Biol Chem
PubMed ID16380372
Human DHRS6 is a previously uncharacterized member of the short chain dehydrogenases/reductase family and displays significant homologies to bacterial hydroxybutyrate dehydrogenases. Substrate screening reveals sole NAD(+)-dependent conversion of (R)-hydroxybutyrate to acetoacetate with K(m) values of about 10 mm, consistent with plasma levels of circulating ketone bodies in situations of starvation ... More
Myxoma virus leukemia-associated protein is responsible for major histocompatibility complex class I and Fas-CD95 down-regulation and defines scrapins, a new group of surface cellular receptor abductor proteins.
AuthorsGuerin JL, Gelfi J, Boullier S, Delverdier M, Bellanger FA, Bertagnoli S, Drexler I, Sutter G, Messud-Petit F
JournalJ Virol
PubMed ID11861858
Down-modulation of major histocompatibility class I (MHC-I) molecules is a viral strategy for survival in the host. Myxoma virus, a member of the Poxviridae family responsible for rabbit myxomatosis, can down-modulate the expression of MHC-I molecules, but the viral factor(s) has not been described. We cloned and characterized a gene ... More
Human loss-of-function gonadotropin-releasing hormone receptor mutants retain wild-type receptors in the endoplasmic reticulum: molecular basis of the dominant-negative effect.
AuthorsBrothers SP, Cornea A, Janovick JA, Conn PM
JournalMol Endocrinol
PubMed ID15105440
The GnRH receptor (GnRHR) is a heptahelical G protein-coupled receptor found in the plasma membrane of pituitary gonadotropes. GnRHR mutants isolated from patients with hypogonadotropic hypogonadism (HH) are frequently mislocalized proteins that can be restored to function by pharmacological chaperones. Nonfunctional HH mutants inhibit ligand binding and ligand-activated second messenger ... More
Two discontinuous segments in the carboxyl terminus are required for membrane targeting of the rat gamma-aminobutyric acid transporter-1 (GAT1).
AuthorsFarhan H, Korkhov VM, Paulitschke V, Dorostkar MM, Scholze P, Kudlacek O, Freissmuth M, Sitte HH
JournalJ Biol Chem
PubMed ID15073174
Like all members of the Na(+)/Cl(-)-dependent neurotransmitter transporter family, the rat gamma-aminobutyric acid transporter-1 (GAT1) is sorted and targeted to specialized domains of the cell surface. Here we identify two discontinuous signals in the carboxyl terminus of GAT1 that cooperate to drive surface expression. This conclusion is based on the ... More
The transmembrane serine protease (TMPRSS3) mutated in deafness DFNB8/10 activates the epithelial sodium channel (ENaC) in vitro.
AuthorsGuipponi M, Vuagniaux G, Wattenhofer M, Shibuya K, Vazquez M, Dougherty L, Scamuffa N, Guida E, Okui M, Rossier C, Hancock M, Buchet K, Reymond A, Hummler E, Marzella PL, Kudoh J, Shimizu N, Scott HS, Antonarakis SE, Rossier BC
JournalHum Mol Genet
PubMed ID12393794
TMPRSS3 encodes a transmembrane serine protease that contains both LDLRA and SRCR domains and is mutated in non-syndromic autosomal recessive deafness (DFNB8/10). To study its function, we cloned the mouse ortholog which maps to Mmu17, which is structurally similar to the human gene and encodes a polypeptide with 88% identity ... More
Translational mobility of the type 3 inositol 1,4,5-trisphosphate receptor Ca2+ release channel in endoplasmic reticulum membrane.
AuthorsFerreri-Jacobia M, Mak DO, Foskett JK
JournalJ Biol Chem
PubMed ID15537642
The inositol 1,4,5-trisphosphate receptor (InsP3R) is an integral membrane protein in the endoplasmic reticulum (ER) which functions as a ligand-gated Ca2+ release channel. InsP3-mediated Ca2+ release modulates the cytoplasmic free Ca2+ concentration ([Ca2+]i), providing a ubiquitous intracellular signal with high temporal and spatial specificity. Precise localization of the InsP3R is ... More
cAMP-induced cytoskeleton rearrangement increases calcium transients through the enhancement of capacitative calcium entry.
AuthorsGrimaldi M, Favit A, Alkon DL
JournalJ Biol Chem
PubMed ID10559242
In this report we investigated the correlation between cell morphology and regulation of cytosolic calcium homeostasis. Type I astrocytes were differentiated to stellate process-bearing cells by a 100-min exposure to cAMP. Differentiation of cortical astrocytes increased the magnitude and duration of calcium transients elicited by phospholipase C-activating agents as measured ... More
Vaccinia virus F1L protein is a tail-anchored protein that functions at the mitochondria to inhibit apoptosis.
AuthorsStewart TL, Wasilenko ST, Barry M
JournalJ Virol
PubMed ID15613337
Members of the poxvirus family encode multiple immune evasion proteins, including proteins that regulate apoptosis. We recently identified one such protein, F1L, encoded by vaccinia virus, the prototypic member of the poxvirus family. F1L localizes to the mitochondria and inhibits apoptosis by interfering with the release of cytochrome c, the ... More
Paclitaxel affects cytosolic calcium signals by opening the mitochondrial permeability transition pore.
AuthorsKidd JF, Pilkington MF, Schell MJ, Fogarty KE, Skepper JN, Taylor CW, Thorn P
JournalJ Biol Chem
PubMed ID11724773
We have characterized the effects of the antimitotic drug paclitaxel (Taxol(TM)) on the Ca(2+) signaling cascade of terminally differentiated mouse pancreatic acinar cells. Using single cell fluorescence techniques and whole-cell patch clamping to record cytosolic Ca(2+) and plasma membrane Ca(2+)-dependent Cl(-) currents, we find that paclitaxel abolishes cytosolic Ca(2+) oscillations ... More
Role of nuclear chromogranin B in inositol 1,4,5-trisphosphate-mediated nuclear Ca2+ mobilization.
AuthorsHuh YH, Chu SY, Park SY, Huh SK, Yoo SH
JournalBiochemistry
PubMed ID16430217
Recently, secretory granule Ca(2+) storage protein chromogranin B (CGB) was shown to be present in the nucleoplasm proper in a complex structure that consists of the inositol 1,4,5-trisphosphate receptor (IP(3)R)/Ca(2+) channels and the phospholipids. Further, the amounts of IP(3)Rs present in the nucleus of bovine chromaffin cells were shown to ... More
Quantitative calcium measurements in subcellular compartments of Plasmodium falciparum-infected erythrocytes.
AuthorsRohrbach P, Friedrich O, Hentschel J, Plattner H, Fink RH, Lanzer M
JournalJ Biol Chem
PubMed ID15927958
The acidic food vacuole exerts several important functions during intraerythrocytic development of the human malarial parasite Plasmodium falciparum. Hemoglobin taken up from the host erythrocyte is degraded in the food vacuole, and the heme liberated during this process is crystallized to inert hemozoin. Several anti-malarial drugs target food vacuolar pathways, ... More
[Ca2+]i signaling between mitochondria and endoplasmic reticulum in neurons is regulated by microtubules. From mitochondrial permeability transition pore to Ca2+-induced Ca2+ release.
AuthorsMironov SL, Ivannikov MV, Johansson M
JournalJ Biol Chem
PubMed ID15516333
The positioning and dynamics of organelles depend on membrane-cytoskeleton interactions. Mitochondria relocate along microtubules (MT), but it is not clear whether MT have direct effects on mitochondrial function. Using two-photon microscopy and the mitochondrial fluorescent dyes rhodamine 123 and Rhod-2, we showed that Taxol and nocodazole, which correspondingly stabilize and ... More
Intercellular calcium waves in HeLa cells expressing GFP-labeled connexin 43, 32, or 26.
This study was undertaken to obtain direct evidence for the involvement of gap junctions in the propagation of intercellular Ca(2+) waves. Gap junction-deficient HeLa cells were transfected with plasmids encoding for green fluorescent protein (GFP) fused to the cytoplasmic carboxyl termini of connexin 43 (Cx43), 32 (Cx32), or 26 (Cx26). ... More
Dengue Virus M Protein Promotes NLRP3 Inflammasome Activation To Induce Vascular Leakage in Mice.
AuthorsPan P, Zhang Q, Liu W, Wang W, Lao Z, Zhang W, Shen M, Wan P, Xiao F, Liu F, Zhang W, Tan Q, Liu X, Wu K, Liu Y, Li G, Wu J
JournalJ Virol
PubMed ID31413130
'Dengue virus (DENV) infection causes serious clinical symptoms, including dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Vascular permeability change is the main feature of the diseases, and the abnormal expression of proinflammatory cytokines is the important cause of vascular permeability change. However, the mechanism underlying vascular permeability induced ... More
TMEM33 regulates intracellular calcium homeostasis in renal tubular epithelial cells.
AuthorsArhatte M, Gunaratne GS, El Boustany C, Kuo IY, Moro C, Duprat F, Plaisant M, Duval H, Li D, Picard N, Couvreux A, Duranton C, Rubera I, Pagnotta S, Lacas-Gervais S, Ehrlich BE, Marchant JS, Savage AM, van Eeden FJM, Wilkinson RN, Demolombe S, Honoré E, Patel A
JournalNat Commun
PubMed ID31048699
'Mutations in the polycystins cause autosomal dominant polycystic kidney disease (ADPKD). Here we show that transmembrane protein 33 (TMEM33) interacts with the ion channel polycystin-2 (PC2) at the endoplasmic reticulum (ER) membrane, enhancing its opening over the whole physiological calcium range in ER liposomes fused to planar bilayers. Consequently, TMEM33 ... More
A novel HIF-2α targeted inhibitor suppresses hypoxia-induced breast cancer stemness via SOD2-mtROS-PDI/GPR78-UPRER axis.
Authors
JournalCell Death Differ
PubMed ID35301432
Cytostatic and Cytotoxic Natural Products against Cancer Cell Models.
AuthorsLing T, Lang WH, Maier J, Quintana Centurion M, Rivas F
JournalMolecules
PubMed ID31130671
'The increasing prevalence of drug resistant and/or high-risk cancers indicate further drug discovery research is required to improve patient outcome. This study outlines a simplified approach to identify lead compounds from natural products against several cancer cell lines, and provides the basis to better understand structure activity relationship of the ... More
TFG is required for autophagy flux and to prevent endoplasmic reticulum stress in CH12 B lymphoma cells.
Authors
JournalAutophagy
PubMed ID32910713
Structural basis for ion selectivity in TMEM175 K+ channels.
Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer.
Authors
JournalAutophagy
PubMed ID28055301
Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis.
Authors
JournalNat Commun
PubMed ID27619642
A conserved, lipid-mediated sorting mechanism of yeast Ist2 and mammalian STIM proteins to the peripheral ER.
Authors
JournalTraffic
PubMed ID19845919
CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4.
Authors
JournalMol Cell
PubMed ID30846317
The cell-wide web coordinates cellular processes by directing site-specific Ca
AuthorsDuan J, Navarro-Dorado J, Clark JH, Kinnear NP, Meinke P, Schirmer EC, Evans AM
JournalNat Commun
PubMed ID31127110
Ca
STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection.
AuthorsWang W, Hu D, Wu C, Feng Y, Li A, Liu W, Wang Y, Chen K, Tian M, Xiao F, Zhang Q, Shereen MA, Chen W, Pan P, Wan P, Wu K, Wu J
JournalPLoS Pathog
PubMed ID32187211
One of the fundamental reactions of the innate immune responses to pathogen infection is the release of pro-inflammatory cytokines, including IL-1ß, processed by the NLRP3 inflammasome. The stimulator of interferon genes (STING) has the essential roles in innate immune response against pathogen infections. Here we reveal a distinct mechanism by ... More