Reactivo Amplex™ Red
Reactivo Amplex™ Red
Citas y referencias (15)
Invitrogen™

Reactivo Amplex™ Red

El reactivo Amplex Red, una sonda altamente sensible y estable para el H2O2, es nuestro mejor sustrato fluorogénico para laMás información
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Número de catálogoCantidadTipo de colorante
A360065 x 1 mgReactivo Amplex UltraRed
A122225 mgReactivo Amplex Red
A2217710 x 10 mgReactivo Amplex Red
Número de catálogo A36006
Precio (MXN)
-
Cantidad:
5 x 1 mg
Tipo de colorante:
Reactivo Amplex UltraRed
El reactivo Amplex Red, una sonda altamente sensible y estable para el H2O2, es nuestro mejor sustrato fluorogénico para la peroxidasa. En presencia de peroxidasa de rábano (HRP), el reactivo Amplex Red reacciona en una estequiometría de 1:1 con H2O2 para producir resorufina altamente fluorescente. Dado que el H2O2 se produce en muchas reacciones enzimáticas diferentes, el reactivo Amplex Red puede usarse para detectar la actividad de muchas enzimas diferentes.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Método de detecciónFluorescente
Tipo de coloranteReactivo Amplex UltraRed
FormularioSólido
Cantidad5 x 1 mg
Condiciones de envíoTemperatura ambiente
SustratoHRP (Horseradish Peroxidase) Substrate
Tipo de sustratoChemical substrate
Aplicación validadaCell Analysis
Para utilizar con (aplicación)Hydrogen Peroxidase Assay
Para utilizar con (equipo)Fluorometer, Microplate Reader
Línea de productosAmplex
Tipo de productoRed Hydrogen Peroxide
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de - 5 a - 30 °C).

Preguntas frecuentes

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (15)

Citations & References
Abstract
A genetically encoded photosensitizer.
Authors:Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA
Journal:Nat Biotechnol
PubMed ID:16369538
'Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, ... More
Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection.
Authors:Zhu A, Romero R, Petty HR,
Journal:Anal Biochem
PubMed ID:20382105
Pyruvate, which is produced in the final step of glycolysis, participates in anabolic metabolism, catabolic metabolism, and signal transduction. We have recently reported a new sensitive and selective fluorimetric assay for pyruvate measurement using Amplex Red as a fluorogenic dye. However, the fluorescence of the reaction product, resorufin, is pH ... More
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution.
Authors:Agresti JJ, Antipov E, Abate AR, Ahn K, Rowat AC, Baret JC, Marquez M, Klibanov AM, Griffiths AD, Weitz DA,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20142500
The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively new studies of complex biological systems and their evolution. This knowledge also drives modern biotechnologies, such as molecular engineering and synthetic biology, which have enormous potential to ... More
Resveratrol promotes clearance of Alzheimer's disease amyloid-beta peptides.
Authors:Marambaud P, Zhao H, Davies P,
Journal:J Biol Chem
PubMed ID:16162502
Several epidemiological studies indicate that moderate consumption of wine is associated with a lower incidence of Alzheimer's disease. Wine is enriched in antioxidant compounds with potential neuroprotective activities. However, the exact molecular mechanisms involved in the beneficial effects of wine intake on the neurodegenerative process in Alzheimer's disease brain remain ... More
Facile SNP detection using bifunctional, cross-linking oligonucleotide probes.
Authors:Peng X, Greenberg MM,
Journal:Nucleic Acids Res
PubMed ID:18281702
A facile, sensitive method for detecting specific sequences of oligonucleotides was developed. Detection of DNA sequences with single nucleotide discrimination is achieved by combining the selectivity of hybridization with an efficient cross-linking reaction. Readily synthesized bifunctional oligonucleotide probes containing a modified pyrimidine that is capable of forming interstrand cross-links under ... More
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