Vybrant® DyeCycle™ Orange Stain

Catalog number: V35005

Molecular Probes™  Related applications: Cell Structure | Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Flow Cytometry

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Vybrant® DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.


Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant® DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2 µL of Vybrant® DyeCycle™ Orange Stain. Final stain concentration is 10 µM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Flow Cytometer
Cell Permeability: Cell-Permeant
Sub-Cellular Localization: Nucleic Acids
Form: Solution
Format: Tube(s)
Solubility: Water
Label or Dye: Vybrant® DyeCycle™ Orange
Product Size: 400 µL
Detection Method: Fluorescent
Number of Reactions: 200
Flow Cytometer Laser Lines: 488, 532
Excitation⁄Emission (nm): 519⁄563
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial of Vybrant® DyeCycle™ orange stain (5 mM in DMSO).

Store at ≤-20°C, desiccate and protect from light.


Manuals & protocols

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