CellTracker Violet BMQC is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The violet excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.
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• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Violet excitation/emission spectra (415/516 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation
The CellTracker Violet BMQC fluorescent dye has been designed to freely pass through cell membranes into cells, where it is is transformed into cell-impermeant reaction products. CellTracker Violet BMQC dye is retained in living cells through several generations. The dye is transferred to daughter cells but not adjacent cells in a population. CellTracker Violet BMQC dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker Violet BMQC dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.
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For Research Use Only. Not for use in diagnostic procedures.