HCS CellMask™ Green stain (H32714) is a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (nucleus and cytoplasm) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in addition to enabling robust cell demarcation. HCS NuclearMask™ stains are also available in a choice of colors for multiplexing flexibility.
Specifications
Color
Green
Excitation Wavelength Range
493⁄516
For Use With (Equipment)
High Content Instrument
Detection Method
Fluorescent
Emission
Visible
Product Line
Molecular Probes™
Shipping Condition
Room Temperature
Product Type
Stain
SubCellular Localization
Nucleus, Cytoplasm & Cytosol
Contents & Storage
1 set of vials, providing sufficient material to stain a total of 10, 96-well microplates.
Sufficient reagent is supplied to perform 10, 96-well plates. Store at ≤-20°C, desiccate and protect from light.
Figures
HeLa Cells labeled with 2 µg/mL HCS CellMask™ Green Stain
Cytochalasin D (CcD) effect on cell size as measured by HCS CellMask™ Blue stain. HeLa control (left) or 10 µM CcD (right) treated for 3 hr before fix/perm. Cells were labeled with HCS CellMask™ Blue stain, phalloidin Alexa Fluor® 488 conjugate for actin (red), mouse anti–a-tubulin IgG (detected with Alexa Fluor® 555 goat anti-mouse IgG, green), and TO-PRO®-3 iodide nuclear stain (magenta). The bar graph shows quantitative differences of cell size as determined by HCS CellMask™ Blue stain.
