HCS CellMask™ Blue Stain

Catalog number: H32720

Related applications:

Cellular Imaging

Description

HCS CellMask™ Blue stain (H32720) replaces the HCS CellMask™ Blue cytoplasmic⁄nuclear stain (H34558) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in addition to enabling robust cell demarcation. HCS NuclearMask™ stains are also available in a choice of colors for multiplexing flexibility.

For Research Use Only. Not for use in diagnostic procedures.

Figures

HeLa Cells labeled with 2 µg/mL HCS CellMask™ Blue Stain

Cytochalasin D (CcD) effect on cell size as measured by HCS CellMask™ Blue stain. HeLa control (left) or 10 µM CcD (right) treated for 3 hr before fix/perm. Cells were labeled with HCS CellMask™ Blue stain, phalloidin Alexa Fluor® 488 conjugate for actin (red), mouse anti–a-tubulin IgG (detected with Alexa Fluor® 555 goat anti-mouse IgG, green), and TO-PRO®-3 iodide nuclear stain (magenta). The bar graph shows quantitative differences of cell size as determined by HCS CellMask™ Blue stain.

Specifications

Color:	Blue
Emission:	Visible
Excitation:	UV
Excitation/Emission:	346⁄442
Shipping Condition:	Room Temperature
Sub Cellular Localization:	Nucleus, Cytoplasm & Cytosol
Product Line:	Molecular Probes®
Detection Method:	Fluorescent
For Use With (Equipment):	High Content Instrument

Contents & storage

1 set of vials, providing sufficient material to stain a total of 10, 96-well microplates.

Sufficient reagent is supplied to perform 10, 96-well plates. Store at ≤-20°C, desiccate and protect from light.