Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells
Invitrogen™
HCS CellMask™ Blue Stain
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Description
HCS CellMask™ Blue stain (H32720) replaces the HCS CellMask™ Blue cytoplasmic⁄nuclear stain (H34558) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in addition to enabling robust cell demarcation. HCS NuclearMask™ stains are also available in a choice of colors for multiplexing flexibility.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Cytochalasin D (CcD) effect on cell size as measured by HCS CellMask™ Blue stain. HeLa control (left) or 10 µM CcD (right) treated for 3 hr before fix/perm. Cells were labeled with HCS CellMask™ Blue stain, phalloidin Alexa Fluor® 488 conjugate for actin (red), mouse anti–a-tubulin IgG (detected with Alexa Fluor® 555 goat anti-mouse IgG, green), and TO-PRO®-3 iodide nuclear stain (magenta). The bar graph shows quantitative differences of cell size as determined by HCS CellMask™ Blue stain.
HeLa Cells labeled with 2 µg/mL HCS CellMask™ Blue Stain
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | High Content Instrument |
| Sub-Cellular Localization: | Cytoplasm & Cytosol, Nucleus |
| Detection Method: | Fluorescent |
| Emission Class: | Visible |
| Excitation Class: | UV |
| Excitation⁄Emission (nm): | 346⁄442 |
| Flow Cytometer Laser Lines: | NA |
| Product Line: | Molecular Probes® |
| Product Size: | 1 set |
| Shipping Condition: | Room Temperature |
Contents & storage
1 set of vials, providing sufficient material to stain a total of 10, 96-well microplates.
Sufficient reagent is supplied to perform 10, 96-well plates. Store at ≤-20°C, desiccate and protect from light.
Sufficient reagent is supplied to perform 10, 96-well plates. Store at ≤-20°C, desiccate and protect from light.
