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Citas y referencias (230)
Invitrogen™
TAMRA, SE; 5-(and-6)-Carboxytetramethylrhodamine, Succinimidyl Ester (5(6)-TAMRA, SE), mixed isomers
La tetrametilrodamina (TMR) es un fluoróforo importante para preparar conjugados de proteínas. Bajo el nombre de TAMRA, el ácido carboxílicoMás información
| Número de catálogo | Cantidad |
|---|---|
| C1171 | 25 mg |
Número de catálogo C1171
Precio (MXN)
-
Cantidad:
25 mg
La tetrametilrodamina (TMR) es un fluoróforo importante para preparar conjugados de proteínas. Bajo el nombre de TAMRA, el ácido carboxílico de la TMR también ha alcanzado prominencia como colorante para el etiquetado de oligonucleótidos y aplicaciones automatizadas de secuenciación de ADN. 5-(y-6)-carboxytetrametilrodamina, éster succinidil es la forma de isómero mixta reactiva a la amina de TAMRA. Formas de isómero único de 5-TAMRA SE (n.º de referencia C-2211) y 6-TAMRA SE (n.º de referencia C-6123) también están disponibles.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Etiqueta o tinteIsómeros TAMRA™, TMR (tetrametilrodamina)
Tipo de productoTAMRA SE
Cantidad25 mg
Fracción reactivaEster activo, succinimidilo éster
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes clásicos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (230)
Citations & References
Abstract
Multiplex detection of single-nucleotide variations using molecular beacons.
Journal:Genetic analysis : biomolecular engineering
PubMed ID:10084107
Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification.
Journal:Clin Chem
PubMed ID:9166227
We describe a PCR-based assay for determining c-erbB-2 oncogene amplification in breast cancer in which we use the TaqMan system. Two fluorogenic probes anneal to the target between primers for c-erbB-2 and beta-globin genes and contain both a reporter dye (6-carboxy-fluorescein) and a quencher dye (6-carboxy-tetramethyl-rhodamine). During the extension phase
DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Journal:Nucleic Acids Res
PubMed ID:1598205
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments which were made single-stranded by digestion with T7 gene 6
Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction.
Journal:Biochemistry
PubMed ID:1591245
We have carried out fluorescence resonance energy transfer (FRET) measurements on four-way DNA junctions in order to analyze the global structure and its dependence on the concentration of several types of ions. A knowledge of the structure and its sensitivity to the solution environment is important for a full understanding
A novel fluorescent toxin to detect and investigate Kv1.3 channel up-regulation in chronically activated T lymphocytes.
Journal:J Biol Chem
PubMed ID:12511563
T lymphocytes with unusually high expression of the voltage-gated Kv1.3 channel (Kv1.3(high) cells) have been implicated in the pathogenesis of experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. We have developed a fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1.3, for detection of Kv1.3(high) cells