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Zitierungen und Referenzen (230)
Invitrogen™
TAMRA, SE; 5-(and-6)-Carboxytetramethylrhodamine, Succinimidyl Ester (5(6)-TAMRA, SE), mixed isomers
Tetramethylrhodamin (TMR) ist ein wichtiges Fluorophor für die Herstellung von Proteinkonjugaten. Unter dem Namen TAMRA ist die Carbonsäure von TMRWeitere Informationen
| Katalognummer | Menge |
|---|---|
| C1171 | 25 mg |
Katalognummer C1171
Preis (EUR)
666,00
Each
Menge:
25 mg
Preis (EUR)
666,00
Each
Tetramethylrhodamin (TMR) ist ein wichtiges Fluorophor für die Herstellung von Proteinkonjugaten. Unter dem Namen TAMRA ist die Carbonsäure von TMR auch ein wichtiger Farbstoff für die Oligonukleotid-Markierung und automatisierte DNA-Sequenzierungsanwendungen. 5-(und-6)-Carboxytetramethylrhodamin, Succinimidylester ist die aminreaktive, gemischte Isomerform von TAMRA. Einzel-Isomerformen von 5-TAMRA SE (Kat.-Nr. C-2211) und 6-TAMRA SE (Kat.-Nr. C-6123) sind ebenfalls erhältlich.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Chemische ReaktivitätAmin
Marker oder FarbstoffTAMRA™ Isomere, TMR (Tetramethylrhodamin)
ProdukttypTAMRA SE
Menge25 mg
Reaktiver TeilAktiv-Ester, Succinimidylester
VersandbedingungRaumtemperatur
MarkertypKlassische Farbstoffe
Unit SizeEach
Inhalt und Lagerung
Bei -5 bis -30 °C lagern und vor Licht schützen.
Zitierungen und Referenzen (230)
Zitierungen und Referenzen
Abstract
Multiplex detection of single-nucleotide variations using molecular beacons.
Journal:Genetic analysis : biomolecular engineering
PubMed ID:10084107
Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification.
Journal:Clin Chem
PubMed ID:9166227
We describe a PCR-based assay for determining c-erbB-2 oncogene amplification in breast cancer in which we use the TaqMan system. Two fluorogenic probes anneal to the target between primers for c-erbB-2 and beta-globin genes and contain both a reporter dye (6-carboxy-fluorescein) and a quencher dye (6-carboxy-tetramethyl-rhodamine). During the extension phase
DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Journal:Nucleic Acids Res
PubMed ID:1598205
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments which were made single-stranded by digestion with T7 gene 6
Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction.
Journal:Biochemistry
PubMed ID:1591245
We have carried out fluorescence resonance energy transfer (FRET) measurements on four-way DNA junctions in order to analyze the global structure and its dependence on the concentration of several types of ions. A knowledge of the structure and its sensitivity to the solution environment is important for a full understanding
A novel fluorescent toxin to detect and investigate Kv1.3 channel up-regulation in chronically activated T lymphocytes.
Journal:J Biol Chem
PubMed ID:12511563
T lymphocytes with unusually high expression of the voltage-gated Kv1.3 channel (Kv1.3(high) cells) have been implicated in the pathogenesis of experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. We have developed a fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1.3, for detection of Kv1.3(high) cells