Invitrogen™

EnzChek™ Caspase-3 Activity Assay Kits

Catalog number: E13183
Invitrogen™

EnzChek™ Caspase-3 Activity Assay Kits

Catalog number: E13183
Enable simple and reliable detection of caspase-3-activity-mediated apoptosis with the EnzChek Caspase-3 Activity Assay Kit, which takes advantage of specially designed substrates that fluoresce upon cleavage by the caspase-3 enzyme. The EnzChek Caspase-3 Activity Assay Kit is available with either the Z-DEVD-AMC or Z-DEVD-R110 substrate, which yields a bright fluorescent product upon proteolytic cleavage by caspase 3/7.
 
Catalog Number
E13183
Unit Size
500 assays
Description
EnzChek Caspase-3 Assay Kit #1, Z-DEVD-AMC substrate
Label or Dye
AMC (7-amino-4-methylcoumarin)
Price (USD)
465.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionEnzChek Caspase-3 Assay Kit #1, Z-DEVD-AMC substrate
FormatCuvets, 96-well Plate
Product TypeCaspase Assay Kit
Excitation/Emission342/441 (cleaved substrate)
No. of Reactions500 assays (reaction volume 100µL per assay)
Shipping ConditionRoom Temperature
Product LineEnzChek
Detection MethodFluorescent
For Use With (Equipment)Fluorometer, Microplate Reader
Label or DyeAMC (7-amino-4-methylcoumarin)
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in freezer (-5 to -30°C) and protect from light.
Unit Size500 assays
Catalog Number
Unit Size
500 assays
Description
EnzChek Caspase-3 Assay Kit #2, Z-DEVD-R110 substrate
Label or Dye
Rhodamine 110
Price (USD)
532.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionEnzChek Caspase-3 Assay Kit #2, Z-DEVD-R110 substrate
FormatCuvets, 96-well Plate
Product TypeCaspase Assay Kit
Excitation/Emission496/520 (cleaved substrate)
No. of Reactions500 assays (reaction volume 100µL per assay)
Shipping ConditionRoom Temperature
Product LineEnzChek
Detection MethodFluorescent
For Use With (Equipment)Fluorometer, Microplate Reader
Label or DyeRhodamine 110
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in freezer (-5 to -30°C) and protect from light.
Unit Size500 assays
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionLabel or DyePrice (USD)AvailabilityQuantity
E13183Full specifications
500 assaysEnzChek Caspase-3 Assay Kit #1, Z-DEVD-AMC substrateAMC (7-amino-4-methylcoumarin)
465.00
Your Price:
***
DescriptionEnzChek Caspase-3 Assay Kit #1, Z-DEVD-AMC substrate
FormatCuvets, 96-well Plate
Product TypeCaspase Assay Kit
Excitation/Emission342/441 (cleaved substrate)
No. of Reactions500 assays (reaction volume 100µL per assay)
Shipping ConditionRoom Temperature
Product LineEnzChek
Detection MethodFluorescent
For Use With (Equipment)Fluorometer, Microplate Reader
Label or DyeAMC (7-amino-4-methylcoumarin)
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in freezer (-5 to -30°C) and protect from light.
Unit Size500 assays
E13184Full specifications
500 assaysEnzChek Caspase-3 Assay Kit #2, Z-DEVD-R110 substrateRhodamine 110
532.00
Your Price:
***
DescriptionEnzChek Caspase-3 Assay Kit #2, Z-DEVD-R110 substrate
FormatCuvets, 96-well Plate
Product TypeCaspase Assay Kit
Excitation/Emission496/520 (cleaved substrate)
No. of Reactions500 assays (reaction volume 100µL per assay)
Shipping ConditionRoom Temperature
Product LineEnzChek
Detection MethodFluorescent
For Use With (Equipment)Fluorometer, Microplate Reader
Label or DyeRhodamine 110
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in freezer (-5 to -30°C) and protect from light.
Unit Size500 assays
Showing 2 of 2
The EnzChek Caspase-3 Activity Assay Kit enables detection of apoptosis by providing a simple and reliable method for assaying caspase-3/7 activity. The kit can be used to continuously measure the activity of caspase-3/7 in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader.

Key features of the EnzChek Caspase-3 Activity Assay Kit includes:
• Fluorescent assay using standard fluorescein (FITC) excitation/emission settings
• Format allows for continuous measurement of caspase-3/7 activity in cell extracts
• Fluorescent and enzymatic controls included

The aminomethylcoumarin (AMC)-derived substrate (Z-aspartic acid-glutamic acid-valine-aspartic acid (DEVD)-AMC) that is included in the EnzChek Caspase-3 Activity Assay Kit is weakly fluorescent in the UV range (excitation/emission maxima ˜330/390 nm), but yields a bright, blue-fluorescent product (excitation/emission maxima ˜342/441 nm) upon proteolytic cleavage. The kit can be used to continuously measure the activity of caspase-3 and closely related proteases in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader.

The rhodamine 110-derived substrate (Z-DEVD-R110) used in the EnzChek Caspase-3 Activity Assay Kit is a non-fluorescent bisamide compound that, upon enzymatic cleavage, is converted in a two-step process to the fluorescent monoamide and then to the even more fluorescent R110 product. Both of these hydrolysis products exhibit spectral properties similar to those of fluorescein, with peak excitation and emission wavelengths of 496 nm and 520 nm, respectively.

In addition to the substrates, the EnzChek Caspase Activity Assay Kit contains the reversible aldehyde inhibitor Ac-DEVD-CHO, as well as an AMC or R110 reference standard. The Ac-DEVD-CHO inhibitor confirms that the fluorescence signals in both induced and control cell populations are due to the activity of the caspase-3/7 protease. The reference standard allows for quantification of the amount of AMC or R110 released in the reaction.
For Research Use Only. Not for use in diagnostic procedures.

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