EnzChek™ Caspase-3 Assay Kit #1, Z-DEVD-AMC substrate

Catalog number:  E13183

 Related applications:

Cell Viability, Proliferation & Function

| Protein Assays and Analysis

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The EnzChek Caspase-3 Assay Kit #1 allows detection of apoptosis by providing a simple and reliable method for assaying caspase-3 activity. The basis for the assay is the aminomethylcoumarin (AMC)-derived substrate Z-DEVD-AMC. This substrate, which is weakly fluorescent in the UV range (excitation/emission maxima ~330/390 nm), yields a bright, blue-fluorescent product (excitation/emission maxima ~342/441 nm) upon proteolytic cleavage. The kit can be used to continuously measure the activity of caspase-3 and closely related proteases in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Fluorescent
For Use With (Equipment): Fluorometer, Microplate Reader
Format: 96-well plate, Cuvettes
Label or Dye: AMC (7-amino-4-methylcoumarin)
Product Line: EnzChek®
Product Size: 500 assays
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols