EnzCheck® Assays provide a rapid and convenient way to measure a range of enzyme activities using fluorescent reporters in a robust assay format. Each assay kit provides the necessary assay buffers, uses a simple protocol and defines the optimum wavelength for sensitive detection. The microplate format is convenient for high throughput analysis using a 200 µL assay volume. We offer assays for proteases, collagenases, elastases, lysozyme, and reverse transcriptase.
Microplate Assays for Enzyme Activity
The EnzChek® Protease Assay Kits contain a casein derivative that is heavily labeled with either the green-fluorescent BODIPY® FL or red-fluorescent BODIPY® TR-X dye. The conjugates typically exhibit <3% of the fluorescence of the corresponding free dyes. Following the assay, the observed increase in fluorescence is directly proportional to protease activity. Because EnzChek® assays do not involve any separation steps, they can be used for continuous measurement.
Other enzyme assays
We've also developed EnzChek® Assay Kits for gelatinase/collagenase, elastase, lysozyme, and reverse transcriptase that are reported by intramolecular self-quenched substrates, allowing continuous, sensitive monitoring of enzyme kinetics.
Assay of porcine pancreatic elastase using the EnzChek® Elastase Assay Kit. Dye-conjugated elastin (25 μg/mL) from the kit was incubated with various concentrations of elastase for the specified incubation times, and fluorescence was measured.
|EnzChek® Protease Assay Kit, Green Fluorescence||EnzChek® Protease Assay Kit, Red Fluorescence|
|Reporter||BODIPY® FL conjugate||BODIPY® TR-X conjugate|
|Usage||Detection limit varies by protease||Detection limit varies by protease|
|Components||Includes assay buffers||Includes assay buffers|
|Format||1,000 assays using 200 µL reaction volume||1,000 assays using 200 µL reaction volume|
|EnzChek® Gelatinase/Collagenase Assay Kit||EnzChek® Elastase Assay Kit||EnzChek® Lysozyme Assay Kit||EnzChek® Reverse Transcriptase Assay Kit|
|Reporter||Fluorescein conjugate||BODIPY®FL Conjugate||Fluorescein conjugate||PicoGreen® reagent|
|Usage||Lower detection limit 2 x 10–4 U/mL||Lower detection limit 5 x 10–3 U/mL||Lower detection limit 20 U/mL||Lower detection limit 2 x 10–3 U/mL|
|Components||Includes assay buffers and controls||Includes assay buffers, inhibitor, and controls||Includes assay buffers and controls||Includes assay buffers|
|Format||250–1,000 assays depending on substrate concentration||600 assays using 200 µL reaction volume||400 assays using 100 µL reaction volume||1,000 assays using 200 µL reaction volume|