Quantitating protein samples is an important step before gel electrophoresis or Western blot analysis, and for measuring bound versus free protein levels in protein binding assays. The choice of assay format is often based on other components in the sample mixture. NanoOrange® is compatible with reducing agents and is sensitive enough that many contaminants can be diluted out below threshold levels. CBQCA is compatible with lipoproteins or lipid protein mixtures.
Microplate Assays for Protein Quantitation
CBQCA Protein Quantitation Kit
The CBQCA Protein Detection Kit accurately detects from 10 ng to 150 mg of protein, lipoprotein or lipid-protein mixtures in 100 to 200 µL. The reagent is essentially non-fluorescent in aqueous solution but reacts with protein to produce highly fluorescent derivatives.
Quant-iT™ Protein Assay Kit
The Quant-iT™ Protein Assay Kit makes protein quantitation easy and accurate, it is highly selective for protein and exhibits very little protein-to-protein variation. Common contaminants, such as salts, solvents, or DNA—but not detergents—are well tolerated in the assay which is performed at room temperature, and the signal is stable for 3 hours.
NanoOrange® Protein Quantitation Kit
NanoOrange® Protein Quantitation Kit provides an ultrasensitive assay for measuring the concentration of proteins in solution with accurate detection between 100 ng/mL and 10 mg/mL using a single dye concentration. The assay is compatible with reducing agents and nucleic acids do not interfere with protein quantitation. Given the sensitivity of the assay, common contaminants such as salts and detergents can generally be diluted below their threshold of interference.
Detection of bovine serum albumin (BSA) using the CBQCA Protein Quantitation Kit. The primary plot shows detection of BSA from 50 ng to 1000 ng. Inset A shows that the detection range can extend up to 150 μg. Inset B shows that the lower detection limit can extend down to 10 ng. Each point is the average of four determinations.
|CBQCA Protein Quantitation Kit||Quant-iT™ Protein Assay Kit||NanoOrange® Protein Quantitation Kit, 200–2000 Assays|
|Reporter||CBQCA||Quant-iT™ Protein Reagent||NanoOrange®|
|Ex/Em (nm)||465/550 nm||470/570 nm||485/590 nm|
|Assay||Quantitation of proteins in the presence of lipids, membrane fractions or detergents||Quantitation of proteins in solution||Quantitation of proteins in solution|
|Usage||Detects at concentrations between 10 ng/mL and 150 ng/mL in 200 µL assay volume||Detects in the range of 0.25–500 µg of protein in 200 µL assay volume||Detects at concentrations between 10 ng/mL and 10 µg/mL in 200 µL assay volume|
|Components||Includes all reagents to prepare stock solution||Includes assay buffer and standards||Includes assay buffer and standards|
|Format||300–800 assays (at 100–200 µL per assay)||1,000 assays, with a 200 µL assay volume||2,000 assays, with a 200 µL assay volume|
- Protein Quantitation in Solution—Molecular Probes® Handbook, Section 9.2
- Introduction to Protein Detection—Molecular Probes® Handbook, Section 9.1
- Learn more about Quant iT™ technology
- Learn more about protein quantification with the Qubit® Fluorometer