A distinctive feature of the early stages of apoptosis is the activation of caspase enzymes, which participate in the cleavage of protein substrates and in the subsequent disassembly of the cell. We offer a series of caspase assays that allow the simple detection of active caspases in living cells in real time or in cellular lysates or extracts.
CellEvent Caspase-3/7 Green Detection Reagent is optimized for apoptosis analysis with a simple no-wash protocol that helps preserve delicate apoptotic cells. Live cells in microplate wells can be monitored in real time for apoptotic activity by measuring fluorescence intensity.
Microplate assay kits for caspase-3/7 measurement provide a choice of substrates with different wavelength emissions. Kits are available using rhodamine 110 or AMC to report caspase activity. Substrates are also available as bulk reagents or predispensed into microplate wells
|CellEvent Caspase-3/7 Green Detection Reagent||EnzChek Caspase-3 Assay Kit #1, Z-DEVD-AMC Substrate||EnzChek Caspase-3 Assay Kit #2, Z-DEVD-R110 Substrate|
|Reporter||DEVD conjugated to a nucleic acid-binding dye||Z-DEVD-AMC substrate||Z-DEVD-R110 substrate|
|Components||Enzyme substrate for live and fixed cells||Includes reference standard and inhibitor||Includes reference standard and inhibitor|
|For use with (Equipment)||Fluorescence microscope, High-content instrument, Microplate readers||Microplate readers||Microplate readers|
|Format||25 µL||100 µL||500 assays using 100 µL reaction volume||500 assays using 100 µL reaction volume|
Expertly detect fluorescence with Thermo Scientific plate readers
High-sensitivity fluorescence detection for 96-1,536 samples can be quickly performed on Fluoroskan or Fluoroskan FL Microplate Fluorometer or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
Molecular Probes Handbook
BioProbes Journal Articles
TaqMan® Arrays for apoptosis
For Research Use Only. Not for use in diagnostic procedures.