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Accurate, efficient nucleic acid quantitation is critical for cellular and molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. These applications include next-generation sequencing, transfection, real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR, northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations.
Quant-iT dsDNA assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. Quant-iT dsDNA assays are routinely used for genotyping by allele-specific PCR, quantitating dsDNA samples before and after PCR amplification, and determining PCR amplification yields before sequencing.
OliGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of ssDNA. The assay is many times more sensitive than conventional UV absorbance, and free nucleotides or short oligonucleotides of up to 6 bases do not interfere with the OliGreen ssDNA quantitation assay. OliGreen reagent assays are routinely used for antisense oligonucleotides, aptamers, genomic DNA isolated under denaturing conditions, PCR primers, sequencing primers, and single-stranded phage DNA.
Quant-iT RNA assays provide a rapid and simple fluorescence-based method for the accurate quantitation of RNA. Quant-iT RNA do not detect significant sample contamination by free nucleotides and thus more accurately measure the amount of intact RNA in potentially degraded samples. Quant-iT RNA assays are ideal for fast and accurate measurement of RNA before generating cDNA, northern blot analysis, S1 nuclease assays, RNase protection assays, reverse transcription PCR, and differential display PCR.
Quant-iT PicoGreen dsDNA Assay Kit | Quant-IT dsDNA Assay Kit, high sensitivity | Quant-iT dsDNA Assay Kit, broad range | Quant-iT 1X dsDNA, high sensitivity | Quant-iT 1X dsDNA, broad range | |
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Target | dsDNA | dsDNA | dsDNA | dsDNA | dsDNA |
Quantitation range | 50 pg–2 μg | 0.2–100 ng | 4–1,000 ng | 0.2–100 ng | 4–4,000 ng |
Ex / Em (nm) | 502/523 | 502/523 | 510/527 | 502/523 | 506/523 |
Components | PicoGreen fluorescent reagent, dilution buffer, and standards | dsDNA HS fluorescent reagent, dilution buffer, and standards | dsDNA BR fluorescent reagent, dilution buffer, and standards | 1X working solution and prediluted standards | 1x working solution and prediluted standards |
Format | 2,000 assays | 1,000 assays | 1,000 assays | 1,000 assays | 1,000 assays |
Protocol outline |
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Cat. No. | P11496 | Q33120 | Q33130 | Q33232 | Q33267 |
Quant-iT OliGreen ssDNA Reagent | Quant-iT OliGreen ssDNA Assay Kit | |
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Target | ssDNA | ssDNA |
Ex / Em (nm) | 500 / 525 | 500 / 525 |
Sample | Selectively quantify oligonucleotides and single-stranded DNA (ssDNA) in solution | Selectively quantify oligonucleotides and single-stranded DNA (ssDNA) in solution |
Quantification range | Detect 200 pg to 2 µg ssDNA in a 200 µL assay volume | Detect 200 pg to 2 µg ssDNA in a 200 µL assay volume |
Components | Bulk reagent | Includes buffer and DNA standard |
Assay format | 2,000 assays using a 200 µL assay volume | 2,000 assays using a 200 µL assay volume |
Protocol outline |
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Cat. No. | O7582 | O11492 |
Quant-iT RiboGreen RNA Assay Kit | Quant-iT RNA Assay Kit | Quant-iT RNA Assay Kit, broad range | Quant-iT RNA Assay Kit, extended range | Quant-iT microRNA Assay Kit | |
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Target | RNA | RNA | RNA | RNA | Small RNA |
Quantitation range | 1–200 ng | 5–100 ng | 20–1,000 ng | 200 ng–10 µg | 1–100 ng |
Ex / Em (nm) | 500 / 525 | 644/673 | 644/673 | 644/673 | 498/518 |
Components | Fluorescent RiboGreen RNA reagent, RNase-free buffer, and rRNA standards | Fluorescent RNA reagent, RNase-free buffer, and rRNA standards | Fluorescent RNA BR reagent, RNase-free BR buffer, and rRNA standards | Fluorescent RNA XR reagent, RNase-free BR buffer, and prediluted tRNA standards | Fluorescent microRNA reagent, RNase-free microRNA buffer, and microRNA standards |
Format | 2,000–20,000 assays | 1,000 assays | 1,000 assays | 1,000 assays | 1,000 assays |
Protocol Outline |
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Cat. No. | R11490 | Q33140 | Q10213 | Q33225 | Q32882 |
Quant-iT PicoGreen ReadyPlate | Quant-iT RiboGreen ReadyPlate | |
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Target | dsDNA | RNA |
Quantitation range | 0.2–200 ng | 1–200 ng |
Ex/Em (nm) | 502/523 | 500/525 |
Components | 96-well plate prefilled with buffer and PicoGreen reagent, prediluted standards | 96-well plate prefilled with buffer and RiboGreen reagent, prediluted standards |
Format | 1 x 96-well ReadyPlate | 1 x 96-well ReadyPlate |
Protocol outline |
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Cat. No. | P32705
| R32705
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Quant-iT PicoGreen dsDNA Assay Kit | Quant-IT dsDNA Assay Kit, high sensitivity | Quant-iT dsDNA Assay Kit, broad range | Quant-iT 1X dsDNA, high sensitivity | Quant-iT 1X dsDNA, broad range | |
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Target | dsDNA | dsDNA | dsDNA | dsDNA | dsDNA |
Quantitation range | 50 pg–2 μg | 0.2–100 ng | 4–1,000 ng | 0.2–100 ng | 4–4,000 ng |
Ex / Em (nm) | 502/523 | 502/523 | 510/527 | 502/523 | 506/523 |
Components | PicoGreen fluorescent reagent, dilution buffer, and standards | dsDNA HS fluorescent reagent, dilution buffer, and standards | dsDNA BR fluorescent reagent, dilution buffer, and standards | 1X working solution and prediluted standards | 1x working solution and prediluted standards |
Format | 2,000 assays | 1,000 assays | 1,000 assays | 1,000 assays | 1,000 assays |
Protocol outline |
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Cat. No. | P11496 | Q33120 | Q33130 | Q33232 | Q33267 |
Quant-iT OliGreen ssDNA Reagent | Quant-iT OliGreen ssDNA Assay Kit | |
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Target | ssDNA | ssDNA |
Ex / Em (nm) | 500 / 525 | 500 / 525 |
Sample | Selectively quantify oligonucleotides and single-stranded DNA (ssDNA) in solution | Selectively quantify oligonucleotides and single-stranded DNA (ssDNA) in solution |
Quantification range | Detect 200 pg to 2 µg ssDNA in a 200 µL assay volume | Detect 200 pg to 2 µg ssDNA in a 200 µL assay volume |
Components | Bulk reagent | Includes buffer and DNA standard |
Assay format | 2,000 assays using a 200 µL assay volume | 2,000 assays using a 200 µL assay volume |
Protocol outline |
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Cat. No. | O7582 | O11492 |
Quant-iT RiboGreen RNA Assay Kit | Quant-iT RNA Assay Kit | Quant-iT RNA Assay Kit, broad range | Quant-iT RNA Assay Kit, extended range | Quant-iT microRNA Assay Kit | |
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Target | RNA | RNA | RNA | RNA | Small RNA |
Quantitation range | 1–200 ng | 5–100 ng | 20–1,000 ng | 200 ng–10 µg | 1–100 ng |
Ex / Em (nm) | 500 / 525 | 644/673 | 644/673 | 644/673 | 498/518 |
Components | Fluorescent RiboGreen RNA reagent, RNase-free buffer, and rRNA standards | Fluorescent RNA reagent, RNase-free buffer, and rRNA standards | Fluorescent RNA BR reagent, RNase-free BR buffer, and rRNA standards | Fluorescent RNA XR reagent, RNase-free BR buffer, and prediluted tRNA standards | Fluorescent microRNA reagent, RNase-free microRNA buffer, and microRNA standards |
Format | 2,000–20,000 assays | 1,000 assays | 1,000 assays | 1,000 assays | 1,000 assays |
Protocol Outline |
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Cat. No. | R11490 | Q33140 | Q10213 | Q33225 | Q32882 |
Quant-iT PicoGreen ReadyPlate | Quant-iT RiboGreen ReadyPlate | |
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Target | dsDNA | RNA |
Quantitation range | 0.2–200 ng | 1–200 ng |
Ex/Em (nm) | 502/523 | 500/525 |
Components | 96-well plate prefilled with buffer and PicoGreen reagent, prediluted standards | 96-well plate prefilled with buffer and RiboGreen reagent, prediluted standards |
Format | 1 x 96-well ReadyPlate | 1 x 96-well ReadyPlate |
Protocol outline |
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Cat. No. | P32705
| R32705
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High-sensitivity fluorescence detection for 6-1,536 samples can be quickly performed on the Varioskan ALF or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents to enable optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
For Research Use Only. Not for use in diagnostic procedures.