Invitrogen™

Quant-iT™ PicoGreen™ dsDNA Reagent

Catalog number:  P7581

Related applications:

Nucleic Acid Quantitation

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Description

Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are:

• Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1)
• Specific for dsDNA in the presence of equimolar amounts of RNA (Figure 2)
• Easy to use-just add the dye to sample, wait 5 minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers and Fluorescence

Applications: The PicoGreen® dsDNA Quantitation Reagent and Kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Microplate Reader
Sample Type: dsDNA
Includes: 1mL in 1 vial of Quant-iT PicoGreen dsDNA reagent (Component A). Reagent is sufficient for 200 assays using an assay volume of 2mL.
Quantity: 1mL
Quantitation Range: 50 pg to 2 µg
Shipping Condition: Room Temperature
Product Line: PICOGREEN®, Quant-iT™

Contents & storage

1 mL in 1 vial - Quant-iT™ PicoGreen® dsDNA reagent (Component A) - Store at 2—6°C, protect from light.
Reagent is sufficient for 200 assays using an assay volume of 2 mL.