Quant-iT™ microRNA Assay Kit
Quant-iT™ microRNA Assay Kit
Invitrogen™

Quant-iT™ microRNA Assay Kit

Quantitate microRNA easily and accurately with the Quant-iT microRNA Assay Kit, a microRNA (miRNA) detection kit that detects miRNA evenRead more
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Catalog number Q32882
Price (USD)/ 1 kit
674.65
Online exclusive
684.00 
Save 9.35 (1%)
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Add to cart
Price (USD)/ 1 kit
674.65
Online exclusive
684.00 
Save 9.35 (1%)
Add to cart
Quant-iT™ microRNA Assay Kit
Catalog numberQ32882
Price (USD)/ 1 kit
674.65
Online exclusive
684.00 
Save 9.35 (1%)
-
Add to cart
Quantitate microRNA easily and accurately with the Quant-iT microRNA Assay Kit, a microRNA (miRNA) detection kit that detects miRNA even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp).

Although not exclusively selective for miRNA, the assay reagent can reproducibly quantify miRNA in pure samples down to levels as low as 0.5 ng following the supplied protocol, even in the presence of mRNA.

Features of the Quant-iT microRNA Assay Kit include:
• Sensitive detection of as little as 0.5 ng miRNA in a microplate well.
• A core dynamic range of 5–500 ng/mL.
• Accurate readings of sample concentrations ranging from 50 ng/mL to 100 μg/mL.

The Quant-iT microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL of sample, mix, and read the fluorescence. The assay is performed at room temperature, and the signal is stable for approximately three hours. Because the assay tolerates 1–20 μL of sample in an assay volume of 200 μL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 μg/mL.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssaymicroRNA Quantitation
Detection MethodFluorescence
Excitation/Emission498/518
For Use With (Equipment)Fluorometer, Microplate Reader
No. of Reactions1000 x 200 μL assays
Quantitation Range1 to 100 ng
Product LineQuant-iT
Quantity1 kit
Unit Size1 kit